FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30523
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATGGGGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAA2690.881302624250565No Hit
GAGTATATGGGGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCC1490.4881564721685287No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA1160.3800412803459686No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTA960.3145169216656292No Hit
GGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAG860.2817547423254595No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC860.2817547423254595No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT860.2817547423254595No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT830.2719260885234086No Hit
GTATCAACGCAGAGTATATGGGGGCGACAAACCTACCGAGCCTGGTGATA790.2588212167873407No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT700.22933525538118793No Hit
ATATGGGGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGA690.22605903744717099No Hit
GTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTTGATTGTAGAT680.222782819513154No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG670.219506601579137No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG640.20967794777708615No Hit
TGCCTGTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTTGATTG610.19984929397503523No Hit
TTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGC610.19984929397503523No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC600.19657307604101826No Hit
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC590.19329685810700128No Hit
ACGCAGAGTATATGGGGGCGACAAACCTACCGAGCCTGGTGATAGCTGGT570.18674442223896734No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA560.18346820430495037No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC540.17691576843691642No Hit
CTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACT490.16053467876683156No Hit
GCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTT490.16053467876683156No Hit
GAGCATGCCTGTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTT480.1572584608328146No Hit
GGTATCAACGCAGAGTATATGGGGGCGACAAACCTACCGAGCCTGGTGAT480.1572584608328146No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC470.15398224289879764No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTT460.15070602496478064No Hit
AACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC450.1474298070307637No Hit
GTACATGGGCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA450.1474298070307637No Hit
TTCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTA450.1474298070307637No Hit
GTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAA440.14415358909674672No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT440.14415358909674672No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCAGAGGAG440.14415358909674672No Hit
TCTCTCTACTGATCAACAGACTACATTTTCACAACTCTTCTTTCCTTTAC440.14415358909674672No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT440.14415358909674672No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA430.14087737116272975No Hit
TATCAACGCAGAGTATATGGGGGCGACAAACCTACCGAGCCTGGTGATAG420.13760115322871277No Hit
GTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGG400.13104871736067883No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC390.12777249942666186No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG380.12449628149264488No Hit
GTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGAT380.12449628149264488No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA370.12122006355862792No Hit
GTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGA350.11466762769059397No Hit
TCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGG340.111391409756577No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT340.111391409756577No Hit
GTACATGGGGATATACAGAAATTCATATAGATGGATTTCTGCCTGTTCTG320.10483897388854307No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCTGCATAAGCC320.10483897388854307No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA310.10156275595452609No Hit
TTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTT310.10156275595452609No Hit
AGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCC310.10156275595452609No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)