FastQCFastQC Report
Wed 8 May 2024
EGAF00001420943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127402
Total Bases12.7 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT47473.726001161677211No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT17361.3626159714918133TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT13021.0219619786188598RNA PCR Primer, Index 3 (95% over 23bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG11700.9183529300952888TruSeq Adapter, Index 3 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGT5550.4356289540195601No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT5030.3948132682375473TruSeq Adapter, Index 3 (96% over 26bp)
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA4340.34065399287295334RNA PCR Primer, Index 3 (96% over 28bp)
GTATTAGTACCATCCCAAGGTTTTTTTCTCATTTAACACATTTGTTTCAT4130.3241707351532943No Hit
ATACAAGAGTCTGTGGCAGTTAAGAATCTGGTTCTGCACTTTGGGAGGCC3550.2786455471656646No Hit
GTATATATGTATATATGGGACATTTTATCTTCTGGCCCAAAGTCAGAACT3390.2660868746173529No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.2653019575830835No Hit
GCATAAAACAGTATGAAGAAGAAAAGTTTAAAAAGAAACAAAAAAGAGTT3380.2653019575830835No Hit
CATATATACAAACCCTAATTCCTACCCTCTTAAATCCTCTAGTTCTAAGA3220.2527432850347718No Hit
CTATTTTACAGGCCAACTTCTTTGCCTCTGAAAATGACAGAGCCAAAATG3090.24253936358926861No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG2970.23312035917803486TruSeq Adapter, Index 3 (95% over 24bp)
TCTTATTACTGGATATATTTGCATTTTACCTCCAGGCCATGTTAGTTCTT2750.2158521844241064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2610.2048633459443337No Hit
TATCTGTACAGCTCAATTTTCACAATAAGTTTAGAATCAGAAAAAAAAAT2530.19858400967017786No Hit
GTACTAATACACTTCGATTATATACATGATCTTTGGGTATTCATGACAAT2520.1977990926359084No Hit
GTACAGATATTCTTTTGATTTCAATTGGGAACATTTGGAAGAACAACAGT2460.19308959043029153No Hit
GTAAAATAGAGTGCTAGAGCTCTTACGCCCCTCCCCTTCTTCCCAACTTC2340.1836705860190578No Hit
GTACATGGGGTCCCCATGGAATACGTTGGCATACCAGAATGCATAAAACA2330.1828856689847883No Hit
GTATATAAATTACATAATACAAGAGTCTGTGGCAGTTAAGAATCTGGTTC2270.17817616677917145No Hit
ATCTAGATGTGTCTCTCTTTAACTATTCTAGGAGTTGATAAAAGGGCTAG2230.17503649864209353No Hit
CATCTAACTAGGTCAAACAACTACCGAGTTCAAATAATTGATATGGAAGT2170.17032699643647667No Hit
ATATAGAGACATATGAATAGTCATAACAGTTTTCAACTTGTTCTTGTTTC2100.16483257719659034No Hit
CTATAAAACCTATATTATGAGAAAGCATTGCTCCTTCAAAGGAATCAAGT2090.16404766016232084No Hit
GTCTTCACTCTAGGCCCAATTTTTACCTGGCCCATGCCTCTAGCCTTTTG2080.16326274312805136No Hit
CCCTAATTCCTACCCTCTTAAATCCTCTAGTTCTAAGAATGGCTTTTTGA2030.15933815795670397No Hit
GTTTTATGTCCGTGTGTCTTCTGTGTCTTCCAAGTTGCTACAGGGCTCTA2030.15933815795670397No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGT1950.15305882168254814No Hit
TCTCTATATTGTACAGGCAAGTAAGACTGTTGTTCTTCCAAATGTTCCCA1950.15305882168254814No Hit
GTTAAAGAGAGACACATCTAGATGGGATGAAAGGTGCCCTAAGCAGGAGA1940.15227390464827867No Hit
CTTTAGCTCTCGCCCCTTTCATTCTCCCCCTGCAAGAACAAAGGCAAAAT1930.1514889876140092No Hit
GTCTTGCTCTGTCGCCTAGGCTGGAGTGCAGTGGCACGATCTCGGCTCAC1930.1514889876140092No Hit
CTTTAACCCCTTCAATTCTCCCTGAAAAGTATTTCTCCATTCATCATCAT1920.15070407057973972No Hit
CTACTGGAACTGCACAAACTGGCCACTGACAAAAATGACCCCCCATTTGT1860.14599456837412286No Hit
CTCTTTAACTATTCTAGGAGTTGATAAAAGGGCTAGGAAGTAGGAAGTTG1860.14599456837412286No Hit
GTTAAATACATTTCCTGGGTAACTTAGGAAATTAAGTTTTTCATAAGGCA1840.1444247343055839No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC1810.14206998320277547No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTT1790.1405001491342365No Hit
CATCTAGATGGGATGAAAGGTGCCCTAAGCAGGAGAAACTGAACAAAAGG1790.1405001491342365No Hit
TCATTGGGGAGTAAGTACTCCTTCCTCGAAAGCTCTTAACGACAGCACAG1780.13971523209996703No Hit
GTCTTGCTCTGTCGCCCAGGTTGGAGTGCAGTGGCGCGATCTCGGCTCAC1750.13736048099715858No Hit
CATATACACTCAGGGGAGCAATACCCTGGACACATAGCTTATTTCACATT1740.1365755639628891No Hit
CAATTAAACCATACTTTTGTAGATTATTTTTCCATGAAGGCAATTTGACA1740.1365755639628891No Hit
TTTATATACAGAACACAAAGCACTTGATTTGCTTAACTGATGTACTTGAT1700.1334358958258112No Hit
GTATAATACAAAAGTCCATTATGAAAAAGTCAAGTCTATTTTGTAACAGG1700.1334358958258112No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC1650.1295113106544638Illumina PCR Primer Index 12 (95% over 24bp)
GTATATAAGACCACGATTTTCTAAAGTGCATATTAGAATTTTTGTTTTTG1610.1263716425173859No Hit
GTATGAAGAAGAAAAGTTTAAAAAGAAACAAAAAAGAGTTGATCAAGAAC1600.12558672548311645No Hit
ATATTGTACAGGCAAGTAAGACTGTTGTTCTTCCAAATGTTCCCAATTGA1600.12558672548311645No Hit
TACCAATACTGGCTAGGCACAATTAGACACATTATTATCTCATTTGCTGT1580.12401689141457747No Hit
ATATAAGACCACGATTTTCTAAAGTGCATATTAGAATTTTTGTTTTTGTA1570.123231974380308No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC1570.123231974380308No Hit
ATTTTTACCTGGCCCATGCCTCTAGCCTTTTGTTCAGTTTCTCCTGCTTA1560.12244705734603852No Hit
TTGTTAGGCTTGTCAAATTGCCTTCATGGAAAAATAATCTACAAAAGTAT1540.12087722327749958No Hit
TTCCTACCCTCTTAAATCCTCTAGTTCTAAGAATGGCTTTTTGACTTGTC1540.12087722327749958No Hit
ATCCTAATGAAATAACAATCTTCCACATAAATTAGGTCACAAAACAAGTA1530.12009230624323008No Hit
GTACATGGGCACTACTGGAACTGCACAAACTGGCCACTGACAAAAATGAC1530.12009230624323008No Hit
GTACAATATAGAGACATATGAATAGTCATAACAGTTTTCAACTTGTTCTT1480.1161677210718827No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT1470.11538280403761324No Hit
GTACAGATGCAAGCGCATTGCACACTTTTTCCTTTCTAAACTTAAAGACA1450.11381296996907428No Hit
GTGTATATGAGTGCTGAGTAGTATTGCAGAGAATGTGATGAGTTATTCAC1450.11381296996907428No Hit
CTCCCAGGGTGTGCTTGTCAAAGAGATATTCCGCCAAGCCAGATTCGGGC1430.1122431359005353No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCT1430.1122431359005353Illumina PCR Primer Index 8 (95% over 21bp)
GCAGTGAACAGTCCCTTGTATAATACAAAAGTCCATTATGAAAAAGTCAA1430.1122431359005353No Hit
GTAATAAGAAGCCCAGATTACTGCAGATAAAAGATTAAAATAGGATTCAA1410.11067330183199635No Hit
GGGTTTCACCATGCTGGCCAGGCTGGTCTCGAACCCCGACCTCGTGATCC1380.10831855072918793No Hit
GTATTGCAGAGAATGTGATGAGTTATTCACTGTCACAACTTTTTCTATAG1380.10831855072918793No Hit
GGTTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAACCTCCACCTCCCG1380.10831855072918793No Hit
GTTTAGAATCAGAAAAAAAAATTTTACTAAATCCTAATGAAATAACAATC1370.10753363369491846No Hit
CTATTAAAGAGCTAAATTCAAGAAATTTGCAAACCAGTACAGCAGTGAAC1350.1059637996263795No Hit
CATTATTATCTCATTTGCTGTATCTGGTATCAAACAACAAAGCCTGAAAG1350.1059637996263795No Hit
TAATAATACAGTTAGCTATTAAAGAGCTAAATTCAAGAAATTTGCAAACC1350.1059637996263795No Hit
TTACAGGTGTGTGCCACAACGCCTGGCTAATTTTTGTATTTTTAGTAGAC1330.10439396555784054No Hit
GACTACAGGCGCGCGCCGCCACGCCCGGCTAATTTTTATATTTTTAGTAG1330.10439396555784054No Hit
GTATTTAAGCAATTTAGGAAAAAAGATATATCACTTACTAAGTTGTTACC1330.10439396555784054No Hit
TGCATGCACTGCCTTGGTGACCAGGGAAGTCACCCCACGGCTATGGGGAA1300.10203921445503211No Hit
CAATTAGACACATTATTATCTCATTTGCTGTATCTGGTATCAAACAACAA1300.10203921445503211No Hit
AGTTAGATGTGGTGTTTATTCAAGTTTGTATGAAATCATGTTTGACAATA1300.10203921445503211No Hit
ATAATACAGTTAGCTATTAAAGAGCTAAATTCAAGAAATTTGCAAACCAG1290.10125429742076263No Hit
GTCATTTTCAGAGGCAAAGAAGTTGGCCTGTAAAATAGAGTGCTAGAGCT1290.10125429742076263No Hit
GTCTTTAAGTTTAGAAAGGAAAAAGTGTGCAATGCGCTTGCATCTGTACT1280.10046938038649315No Hit
CCCTTGTATAATACAAAAGTCCATTATGAAAAAGTCAAGTCTATTTTGTA1280.10046938038649315No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)