FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001420944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127402
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGTACCATCCCAAGGTTTTTTTCTCATTTAACACATTTGTTTCAT5070.3979529363746252No Hit
GTATATATGTATATATGGGACATTTTATCTTCTGGCCCAAAGTCAGAACT3750.29434388785105414No Hit
CATATATACAAACCCTAATTCCTACCCTCTTAAATCCTCTAGTTCTAAGA3430.26922654275443086No Hit
ATACAAGAGTCTGTGGCAGTTAAGAATCTGGTTCTGCACTTTGGGAGGCC3300.2590226213089276No Hit
GCATAAAACAGTATGAAGAAGAAAAGTTTAAAAAGAAACAAAAAAGAGTT3290.2582377042746582No Hit
CTATTTTACAGGCCAACTTCTTTGCCTCTGAAAATGACAGAGCCAAAATG3040.23861477841792123No Hit
GTACAGATATTCTTTTGATTTCAATTGGGAACATTTGGAAGAACAACAGT2800.21977676959545378No Hit
GTAAAATAGAGTGCTAGAGCTCTTACGCCCCTCCCCTTCTTCCCAACTTC2740.21506726738983692No Hit
GTACTAATACACTTCGATTATATACATGATCTTTGGGTATTCATGACAAT2680.21035776518422No Hit
CATCTAACTAGGTCAAACAACTACCGAGTTCAAATAATTGATATGGAAGT2570.20172367780725578No Hit
TATCTGTACAGCTCAATTTTCACAATAAGTTTAGAATCAGAAAAAAAAAT2560.2009387607729863No Hit
TCTTATTACTGGATATATTTGCATTTTACCTCCAGGCCATGTTAGTTCTT2540.19936892670444734No Hit
GTACATGGGGTCCCCATGGAATACGTTGGCATACCAGAATGCATAAAACA2490.19544434153309995No Hit
GTCTTCACTCTAGGCCCAATTTTTACCTGGCCCATGCCTCTAGCCTTTTG2310.18131583491624934No Hit
GTCTTGCTCTGTCGCCTAGGCTGGAGTGCAGTGGCACGATCTCGGCTCAC2220.17425158160782406No Hit
TCTCTATATTGTACAGGCAAGTAAGACTGTTGTTCTTCCAAATGTTCCCA2200.1726817475392851No Hit
CCCTAATTCCTACCCTCTTAAATCCTCTAGTTCTAAGAATGGCTTTTTGA2120.16640241126512928No Hit
GTTTTATGTCCGTGTGTCTTCTGTGTCTTCCAAGTTGCTACAGGGCTCTA2120.16640241126512928No Hit
ATCTAGATGTGTCTCTCTTTAACTATTCTAGGAGTTGATAAAAGGGCTAG2110.1656174942308598No Hit
GTATATAAATTACATAATACAAGAGTCTGTGGCAGTTAAGAATCTGGTTC2080.16326274312805136No Hit
GTTAAATACATTTCCTGGGTAACTTAGGAAATTAAGTTTTTCATAAGGCA2040.16012307499097345No Hit
CATCTAGATGGGATGAAAGGTGCCCTAAGCAGGAGAAACTGAACAAAAGG2020.1585532409224345No Hit
ATATAGAGACATATGAATAGTCATAACAGTTTTCAACTTGTTCTTGTTTC1950.15305882168254814No Hit
CTATAAAACCTATATTATGAGAAAGCATTGCTCCTTCAAAGGAATCAAGT1910.14991915354547025No Hit
GTATAATACAAAAGTCCATTATGAAAAAGTCAAGTCTATTTTGTAACAGG1910.14991915354547025No Hit
GTTAAAGAGAGACACATCTAGATGGGATGAAAGGTGCCCTAAGCAGGAGA1880.1475644024426618No Hit
CTACTGGAACTGCACAAACTGGCCACTGACAAAAATGACCCCCCATTTGT1830.14363981727131442No Hit
CAATTAAACCATACTTTTGTAGATTATTTTTCCATGAAGGCAATTTGACA1830.14363981727131442No Hit
GTCTTGCTCTGTCGCCCAGGTTGGAGTGCAGTGGCGCGATCTCGGCTCAC1820.14285490023704495No Hit
TTGTTAGGCTTGTCAAATTGCCTTCATGGAAAAATAATCTACAAAAGTAT1800.14128506616850597No Hit
GTACAGATGCAAGCGCATTGCACACTTTTTCCTTTCTAAACTTAAAGACA1770.13893031506569756No Hit
CTTTAGCTCTCGCCCCTTTCATTCTCCCCCTGCAAGAACAAAGGCAAAAT1770.13893031506569756No Hit
CTCTTTAACTATTCTAGGAGTTGATAAAAGGGCTAGGAAGTAGGAAGTTG1760.13814539803142808No Hit
CTTTAACCCCTTCAATTCTCCCTGAAAAGTATTTCTCCATTCATCATCAT1680.13186606175727225No Hit
GTAATAAGAAGCCCAGATTACTGCAGATAAAAGATTAAAATAGGATTCAA1660.1302962276887333No Hit
GTATTTAAGCAATTTAGGAAAAAAGATATATCACTTACTAAGTTGTTACC1650.1295113106544638No Hit
GAAAATGACAGAGCCAAAATGAAATAAAAACCTTGCTTTTACAAATATTT1630.12794147658592486No Hit
GTATGAAGAAGAAAAGTTTAAAAAGAAACAAAAAAGAGTTGATCAAGAAC1600.12558672548311645No Hit
ATCCTAATGAAATAACAATCTTCCACATAAATTAGGTCACAAAACAAGTA1600.12558672548311645No Hit
ATAATACAGTTAGCTATTAAAGAGCTAAATTCAAGAAATTTGCAAACCAG1600.12558672548311645No Hit
ATATTGTACAGGCAAGTAAGACTGTTGTTCTTCCAAATGTTCCCAATTGA1590.12480180844884696No Hit
TCATTGGGGAGTAAGTACTCCTTCCTCGAAAGCTCTTAACGACAGCACAG1580.12401689141457747No Hit
GTATATAAGACCACGATTTTCTAAAGTGCATATTAGAATTTTTGTTTTTG1580.12401689141457747No Hit
GTTTAAATGCTTGTAACAAACGTGTTATCCAAAACAGCACAATATGATGA1570.123231974380308No Hit
ATTCTAAACTTATTGTGAAAATTGAGCTGTACAGATATTCTTTTGATTTC1540.12087722327749958No Hit
TTACAGGTGTGTGCCACAACGCCTGGCTAATTTTTGTATTTTTAGTAGAC1520.11930738920896061No Hit
ATGTAAGACAATCAATTAAACCATACTTTTGTAGATTATTTTTCCATGAA1490.11695263810615217No Hit
ATATAAGACCACGATTTTCTAAAGTGCATATTAGAATTTTTGTTTTTGTA1490.11695263810615217No Hit
GTACATGGGCACTACTGGAACTGCACAAACTGGCCACTGACAAAAATGAC1480.1161677210718827No Hit
CTATTAAAGAGCTAAATTCAAGAAATTTGCAAACCAGTACAGCAGTGAAC1470.11538280403761324No Hit
CATATACACTCAGGGGAGCAATACCCTGGACACATAGCTTATTTCACATT1460.11459788700334374No Hit
GTGTATATGAGTGCTGAGTAGTATTGCAGAGAATGTGATGAGTTATTCAC1460.11459788700334374No Hit
CAATTAGACACATTATTATCTCATTTGCTGTATCTGGTATCAAACAACAA1450.11381296996907428No Hit
TACCAATACTGGCTAGGCACAATTAGACACATTATTATCTCATTTGCTGT1430.1122431359005353No Hit
TTATTATCTCATTTGCTGTATCTGGTATCAAACAACAAAGCCTGAAAGAT1420.11145821886626585No Hit
ATCCAAAACAGCACAATATGATGATGCTAAGACTTACAAAATTAACCTAA1410.11067330183199635No Hit
GTATATGAGTGCTGAGTAGTATTGCAGAGAATGTGATGAGTTATTCACTG1400.10988838479772689No Hit
GTATTGCAGAGAATGTGATGAGTTATTCACTGTCACAACTTTTTCTATAG1360.10674871666064897No Hit
CTTAAATCCTCTAGTTCTAAGAATGGCTTTTTGACTTGTCTTTAAGTTTA1360.10674871666064897No Hit
CTTAAAAAAGTGATAGCTCAGAAATATTTGTAAAAGCAAGGTTTTTATTT1350.1059637996263795No Hit
TCACCATGCTGGCCAGGCTGGTCTCGAACCCCGACCTCGTGATCCGCCCT1340.10517888259211No Hit
CTTCAATTCTCCCTGAAAAGTATTTCTCCATTCATCATCATTGGGGAGTA1330.10439396555784054No Hit
TTTATATACAGAACACAAAGCACTTGATTTGCTTAACTGATGTACTTGAT1330.10439396555784054No Hit
GCCTAACAAAGACCAAGTTGTTCAAACTATGTTTCTAGGAATATAGTTTA1330.10439396555784054No Hit
TTCCTACCCTCTTAAATCCTCTAGTTCTAAGAATGGCTTTTTGACTTGTC1320.10360904852357104No Hit
GGGTTAAAGTGTTAAATATGTTGCCTAGACAAGGGTTCTTTAAAGAAAGA1320.10360904852357104No Hit
GTCATTTTCAGAGGCAAAGAAGTTGGCCTGTAAAATAGAGTGCTAGAGCT1320.10360904852357104No Hit
GAATAATTGAATCCTATTTTAATCTTTTATCTGCAGTAATCTGGGCTTCT1310.10282413148930158No Hit
CTATTTGATCTGAGAAACTGACTGCTGTTTACATCCAGGTATGCAATGTG1310.10282413148930158No Hit
GTTGTTACCAGGGAAAATTATCATGTAAGACAATCAATTAAACCATACTT1310.10282413148930158No Hit
GCAGTAATCTGGGCTTCTTATTACTGGATATATTTGCATTTTACCTCCAG1300.10203921445503211No Hit
CTCCCAGGGTGTGCTTGTCAAAGAGATATTCCGCCAAGCCAGATTCGGGC1290.10125429742076263No Hit
GGGTTTCACCATGCTGGCCAGGCTGGTCTCGAACCCCGACCTCGTGATCC1280.10046938038649315No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)