FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94044
Sequences flagged as poor quality0
Sequence length100
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4000.4253328229339458No Hit
CTTATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTG2310.2456297052443537No Hit
CATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTCGCCGT2190.23286972055633534Illumina RNA PCR Primer (96% over 25bp)
ATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTC1480.15737314448555995No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)