FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001420959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328624
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT198171.491543130336348No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT88480.6659521429689664RNA PCR Primer, Index 23 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74410.5600531075759583No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT66080.4973566637363167RNA PCR Primer, Index 23 (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54350.409069834656005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34090.25658124495718876No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG25200.18966991413673093RNA PCR Primer, Index 23 (96% over 30bp)
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT16590.1248660268066812RNA PCR Primer, Index 23 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG15610.11748997459025277Illumina PCR Primer Index 1 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)