FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23154
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT366415.824479571564309No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG13365.770061328496156TruSeq Adapter, Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT13135.670726440355878TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT9924.284356914571996RNA PCR Primer, Index 10 (95% over 23bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA4912.1205839163859377RNA PCR Primer, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGT4221.8225792519651032No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT3831.6541418329446316TruSeq Adapter, Index 10 (96% over 26bp)
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG2200.9501597996026604TruSeq Adapter, Index 10 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGT1640.7083009415219833No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTT1500.647836227001814No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC1480.6391984106417897No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC1390.6003282370216809Illumina Paired End PCR Primer 2 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT1060.45780426708128186No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC1000.43189081800120926No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.4146151852811609No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGACCTCGTATGCCGT950.4102962771011488No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCT940.4059773689211368Illumina PCR Primer Index 5 (95% over 21bp)
TCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCTGCTTG900.38870173620108833Illumina PCR Primer Index 5 (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.3757450116610521No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGT780.33687483804094326No Hit
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC720.31096138896087067No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGAGGCT690.29800466442083445No Hit
GTACATGGGCCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTA660.28504793988079813No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTT640.27641012352077393No Hit
GTTCTTGGGTGGGTGTGGGTATTATACTAAGTTGAGATGATATCATTTAC510.22026431718061676No Hit
GTATTATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG500.21594540900060463No Hit
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAA470.2029886844605684TruSeq Adapter, Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTTCT460.19866977628055627Illumina PCR Primer Index 5 (95% over 21bp)
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTT450.19435086810054417No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTT440.1900319599205321TruSeq Adapter, Index 10 (96% over 27bp)
CTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC430.18571305174052No Hit
TCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTTCTGCTTG420.1813941435605079Illumina PCR Primer Index 5 (96% over 26bp)
GTATCAACGCAGAGTACATGGGCCTCGATGTTGGATCAGGACATCCCGAT420.1813941435605079No Hit
ATATTATCCTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGC410.17707523538049583No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGAGGC410.17707523538049583No Hit
ATCCTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGCACCAC390.16843741902047163No Hit
CCCTACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTA380.16411851084045953No Hit
ATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTA370.15979960266044743No Hit
GAGTACATGGGCCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGC370.15979960266044743No Hit
CCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGCCATT360.15548069448043533No Hit
CTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCTGCTTGA360.15548069448043533Illumina PCR Primer Index 5 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGACCTCGTATGCCGTCTT360.15548069448043533No Hit
CTCTCAATCTTATCCATCATAGCAGGCAGTTGAGGTGGATTAAACCAAAC350.15116178630042326No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCGAGGCT350.15116178630042326No Hit
GTACATGGGCTCCCTCACTAAACGTAAGCCTTCTCCTCACTCTCTCAATC350.15116178630042326No Hit
GTATAAAAGGGGAGATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTG330.14252396994039906No Hit
TGTTAGGGTTGTACGGTAGAACTGCTATTATTCATCCTATGTGGGTAATT330.14252396994039906No Hit
TTCTTAATCTAACTATTTATATTATCCTAACTACTACCGCATTCCTACTA330.14252396994039906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.13388615358037487No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAAATCGTATGCCGT310.13388615358037487No Hit
TCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGT280.12092942904033861No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGTCTTCT280.12092942904033861Illumina PCR Primer Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGTCTT280.12092942904033861No Hit
GATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATTAGGCGTAGGTAGAA270.1166105208603265No Hit
GAGTACATGGGCTCCCTCACTAAACGTAAGCCTTCTCCTCACTCTCTCAA270.1166105208603265No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCTGCT270.1166105208603265Illumina PCR Primer Index 5 (95% over 24bp)
CCCTTAATTCCATCCACCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAAC270.1166105208603265No Hit
CTACTACCGCATTCCTACTACTCAACTTAAACTCCAGCACCACGACCCTA260.11229161268031443No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCCGATCTCGTATGCCGT260.11229161268031443No Hit
ACCCTAACATAACCATTCTTAATCTAACTATTTATATTATCCTAACTACT260.11229161268031443No Hit
GTAGTAGGGTCGTGGTGCTGGAGTTTAAGTTGAGTAGTAGGAATGCGGTA250.10797270450030232No Hit
GGTTAAGGAGGGTGATGGTGGCTATGATGGTGGGGATGATGAGGCTATTG250.10797270450030232No Hit
GTTAGATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATTAGGCGTAGGT240.10365379632029023No Hit
ATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCA240.10365379632029023No Hit
GTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTA240.10365379632029023No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)