FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23154
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGCCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTA760.32823702168091906No Hit
CCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGCCATT590.25481558262071347No Hit
GTATTATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG530.22890213354064093No Hit
GATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATTAGGCGTAGGTAGAA480.20730759264058046No Hit
GTTCTTGGGTGGGTGTGGGTATTATACTAAGTTGAGATGATATCATTTAC480.20730759264058046No Hit
GTACATGGGCTCCCTCACTAAACGTAAGCCTTCTCCTCACTCTCTCAATC450.19435086810054417No Hit
GAGTACATGGGCCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGC420.1813941435605079No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTT410.17707523538049583No Hit
ATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTA390.16843741902047163No Hit
GTTAGATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATTAGGCGTAGGT380.16411851084045953No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.15116178630042326No Hit
CCCTACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTA350.15116178630042326No Hit
CTCTCAATCTTATCCATCATAGCAGGCAGTTGAGGTGGATTAAACCAAAC350.15116178630042326No Hit
TGTTAGGGTTGTACGGTAGAACTGCTATTATTCATCCTATGTGGGTAATT340.14684287812041116No Hit
ATATTATCCTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGC340.14684287812041116No Hit
ATCCTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGCACCAC320.13820506176038697No Hit
GATTAGGCGTAGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATGA290.1252483372203507No Hit
GGTGTGGGTATTATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCG280.12092942904033861No Hit
CCCTTAATTCCATCCACCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAAC280.12092942904033861No Hit
GTATCAACGCAGAGTACATGGGCCTCGATGTTGGATCAGGACATCCCGAT260.11229161268031443No Hit
ACCCTAACATAACCATTCTTAATCTAACTATTTATATTATCCTAACTACT260.11229161268031443No Hit
ATTGAGGAGTATGCTAAGATTTTGCGTAGCTGGGTTTGGTTTAATCCACC250.10797270450030232No Hit
ACGCAGAGTACATGGGCCTCGATGTTGGATCAGGACATCCCGATGGTGCA250.10797270450030232No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC250.10797270450030232No Hit
ATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCA250.10797270450030232No Hit
TTGTTAGATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATTAGGCGTAG240.10365379632029023No Hit
CTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACAT240.10365379632029023No Hit
GGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATGATGGTGGGGATG240.10365379632029023No Hit
GTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTA240.10365379632029023No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)