FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14216
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT295120.75830050647158No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT10537.407146876758581TruSeq Adapter, Index 3 (95% over 21bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG9056.366066404051772TruSeq Adapter, Index 3 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT7145.022509848058525RNA PCR Primer, Index 3 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGT3962.7855936972425437No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT3252.286156443444007TruSeq Adapter, Index 3 (96% over 26bp)
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA2751.9344400675295441RNA PCR Primer, Index 3 (96% over 28bp)
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG1841.2943162633652223TruSeq Adapter, Index 3 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTT1441.0129431626336522No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGT1400.9848058525604951No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC1050.7386043894203714Illumina PCR Primer Index 12 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC1000.7034327518289252No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCT990.6963984243106359Illumina PCR Primer Index 8 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAACCTCGTATGCCGT890.6260551491277434No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC780.5486775464265616No Hit
TCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCTTG720.5064715813168261Illumina PCR Primer Index 8 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT630.44316263365222286No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTT510.35875070343275184No Hit
CTTTACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC490.34468204839617334No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAGG460.3235790658413056No Hit
ATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTT450.31654473832301633TruSeq Adapter, Index 3 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.28137310073157007No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAG350.24620146314012378No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTTCT350.24620146314012378Illumina PCR Primer Index 8 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC340.23916713562183453No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.23916713562183453No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATATCGTATGCCGT330.2321328081035453No Hit
CTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCTTGA310.2180641530669668Illumina PCR Primer Index 8 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTC280.19696117051209902No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACGTAGAGG270.1899268429938098No Hit
TCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTTCTGCTTG260.18289251547552052Illumina PCR Primer Index 8 (96% over 26bp)
CACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTG220.15475520540236354Illumina PCR Primer Index 8 (95% over 22bp)
CATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCT220.15475520540236354Illumina PCR Primer Index 8 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAACCTCGTATGCCGTCTT210.1477208778840743No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAAATCGTATGCCGT200.14068655036578503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.12661789532920653No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCC170.11958356781091727No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATATCGTATGCCGTCTT160.11254924029262803No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG160.11254924029262803RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACGTAGAGGAATATCGTATGCCGTCTTCTGCTTG150.10551491277433878Illumina PCR Primer Index 8 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)