FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20316
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT474123.33628667060445No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT15467.609765701909825RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT11505.660563102973026RNA PCR Primer, Index 27 (96% over 25bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG11375.596574128765505RNA PCR Primer, Index 27 (96% over 30bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT4502.215002953337271RNA PCR Primer, Index 27 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGT3691.8163024217365622No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA3221.5849576688324474RNA PCR Primer, Index 27 (96% over 30bp)
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG2651.304390628076393RNA PCR Primer, Index 27 (100% over 24bp)
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGC1690.8318566646977752TruSeq Adapter, Index 27 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGT1640.8072455207718056No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC1580.7777121480606419No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT1430.7038787162827328RNA PCR Primer, Index 27 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC1290.6349675132900177No Hit
GTATAATCAGGTCTTTATTCAAAAGAAGCTGTCCAAAATGATTTGACCTT1190.5857452254380784No Hit
GATGAGAAGGCGGCGGTTGCAGGCAAGAAGCCTGTGGTAGGTAAGAAAGG1070.5266784800157511No Hit
GTATCAACGCAGATGAGAAGGCGGCGGTTGCAGGCAAGAAGCCTGTGGTA1070.5266784800157511No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGTCTT1040.5119117936601694No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTACCTCGTATGCCGT1000.49222287851939356No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.41838944674148454No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGTCTTCT840.4134672179562906RNA PCR Primer, Index 38 (95% over 21bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGGACTCC840.4134672179562906No Hit
GGTATCAACGCAGATGAGAAGGCGGCGGTTGCAGGCAAGAAGCCTGTGGT800.39377830281551485No Hit
ACGCAGATGAGAAGGCGGCGGTTGCAGGCAAGAAGCCTGTGGTAGGTAAG770.3790116164599331No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.3396337861783816No Hit
CTTTACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC680.33471155739318764RNA PCR Primer, Index 27 (95% over 21bp)
GTGTTAAGAAGCAGAAGAAGCCTCTGGTGGGAAAAAAGGCAGCAGCTACC640.3150226422524119No Hit
GTAGTAGGTTTCTTCTCTGCAGGCTTCTTTTCAGGGGCTGGTTTCTTGGT640.3150226422524119No Hit
GTCCAAAATGATTTGACCTTTATGGAATAATCAAATTTAAGAGTTTATGC540.2658003544004725No Hit
GTTTCTCACTGCCTGTATAATCAGGTCTTTATTCAAAAGAAGCTGTCCAA540.2658003544004725No Hit
ATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTT530.2608781256152786RNA PCR Primer, Index 27 (100% over 27bp)
TTATGGAATAATCAAATTTAAGAGTTTATGCAGCAGGCTTCTTCTCCTCT520.2559558968300847No Hit
CTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC480.23626698168930893No Hit
GGCAAGAAGCCTGTGGTAGGTAAGAAAGGAAAGAAGGCTGCTGTTGGTGT470.231344752904115No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATATCGTATGCCGT470.231344752904115No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGTCTT440.21657806654853318No Hit
TATCAACGCAGATGAGAAGGCGGCGGTTGCAGGCAAGAAGCCTGTGGTAG440.21657806654853318No Hit
CTGTAGTAGGTTTCTTCTCTGCAGGCTTCTTTTCAGGGGCTGGTTTCTTG440.21657806654853318No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGTCTTCT420.2067336089781453RNA PCR Primer, Index 38 (95% over 21bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGGACTC420.2067336089781453No Hit
TCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGTCTTCTGCTTG410.2018113801929514RNA PCR Primer, Index 38 (96% over 26bp)
CTGTATAATCAGGTCTTTATTCAAAAGAAGCTGTCCAAAATGATTTGACC400.19688915140775742No Hit
AGTTTATGCAGCAGGCTTCTTCTCCTCTGTAGTAGGTTTCTTCTCTGCAG390.19196692262256349No Hit
GTGGTAGGTAAGAAAGGAAAGAAGGCTGCTGTTGGTGTTAAGAAGCAGAA380.18704469383736955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.18212246505217564No Hit
CTCACTGCCTGTATAATCAGGTCTTTATTCAAAAGAAGCTGTCCAAAATG360.1772002362669817No Hit
GGAAAGAAGGCTGCTGTTGGTGTTAAGAAGCAGAAGAAGCCTCTGGTGGG360.1772002362669817No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACGGACTCC340.16735577869659382No Hit
GAAGAAGCCTCTGGTGGGAAAAAAGGCAGCAGCTACCAAGAAACCAGCCC330.16243354991139988No Hit
CAAAAGAAGCTGTCCAAAATGATTTGACCTTTATGGAATAATCAAATTTA320.15751132112620594No Hit
GTAAGAAAGGAAAGAAGGCTGCTGTTGGTGTTAAGAAGCAGAAGAAGCCT310.152589092341012No Hit
CCTCTGTAGTAGGTTTCTTCTCTGCAGGCTTCTTTTCAGGGGCTGGTTTC300.14766686355581807No Hit
ATTCAAAAGAAGCTGTCCAAAATGATTTGACCTTTATGGAATAATCAAAT300.14766686355581807No Hit
CATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGTCTTCTGCT290.14274463477062413RNA PCR Primer, Index 38 (95% over 24bp)
AATCAGGTCTTTATTCAAAAGAAGCTGTCCAAAATGATTTGACCTTTATG290.14274463477062413No Hit
CCTTTATGGAATAATCAAATTTAAGAGTTTATGCAGCAGGCTTCTTCTCC290.14274463477062413No Hit
CAAGAAGCCTGTGGTAGGTAAGAAAGGAAAGAAGGCTGCTGTTGGTGTTA270.13290017720023625No Hit
GCCTGTGGTAGGTAAGAAAGGAAAGAAGGCTGCTGTTGGTGTTAAGAAGC270.13290017720023625No Hit
GATTTGACCTTTATGGAATAATCAAATTTAAGAGTTTATGCAGCAGGCTT260.12797794841504234No Hit
ATTTAAGAGTTTATGCAGCAGGCTTCTTCTCCTCTGTAGTAGGTTTCTTC260.12797794841504234No Hit
TGAGAAGGCGGCGGTTGCAGGCAAGAAGCCTGTGGTAGGTAAGAAAGGAA260.12797794841504234No Hit
GGTCTTTATTCAAAAGAAGCTGTCCAAAATGATTTGACCTTTATGGAATA250.12305571962984839No Hit
GAAAAGAAGCCTGCAGAGAAGAAACCTACTACAGAGGAGAAGAAGCCTGC250.12305571962984839No Hit
GCCTGTATAATCAGGTCTTTATTCAAAAGAAGCTGTCCAAAATGATTTGA250.12305571962984839No Hit
CAACGCAGATGAGAAGGCGGCGGTTGCAGGCAAGAAGCCTGTGGTAGGTA240.11813349084465447No Hit
GAAGAAACCTACTACAGAGGAGAAGAAGCCTGCTGCATAAACTCTTAAAT240.11813349084465447No Hit
CTTCTTTTCAGGGGCTGGTTTCTTGGTAGCTGCTGCCTTTTTTCCCACCA240.11813349084465447No Hit
GGCTTCTTCTCCTCTGTAGTAGGTTTCTTCTCTGCAGGCTTCTTTTCAGG230.11321126205946053No Hit
CTGTTGGTGTTAAGAAGCAGAAGAAGCCTCTGGTGGGAAAAAAGGCAGCA230.11321126205946053No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTACCTCGTATGCCGTCTT220.10828903327426659No Hit
GGTTTCTTCTCTGCAGGCTTCTTTTCAGGGGCTGGTTTCTTGGTAGCTGC220.10828903327426659No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTTCTATCTCGTATGCCGT210.10336680448907265No Hit
TGTATAATCAGGTCTTTATTCAAAAGAAGCTGTCCAAAATGATTTGACCT210.10336680448907265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)