FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20316
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATCAGGTCTTTATTCAAAAGAAGCTGTCCAAAATGATTTGACCTT1420.6989564874975389No Hit
GATGAGAAGGCGGCGGTTGCAGGCAAGAAGCCTGTGGTAGGTAAGAAAGG1290.6349675132900177No Hit
GTATCAACGCAGATGAGAAGGCGGCGGTTGCAGGCAAGAAGCCTGTGGTA1020.5020673360897815No Hit
GGTATCAACGCAGATGAGAAGGCGGCGGTTGCAGGCAAGAAGCCTGTGGT960.47253396337861786No Hit
ACGCAGATGAGAAGGCGGCGGTTGCAGGCAAGAAGCCTGTGGTAGGTAAG790.38885607403032096No Hit
GTAGTAGGTTTCTTCTCTGCAGGCTTCTTTTCAGGGGCTGGTTTCTTGGT690.3396337861783816No Hit
GTCCAAAATGATTTGACCTTTATGGAATAATCAAATTTAAGAGTTTATGC630.31010041346721795No Hit
GTTTCTCACTGCCTGTATAATCAGGTCTTTATTCAAAAGAAGCTGTCCAA610.30025595589683013No Hit
TATCAACGCAGATGAGAAGGCGGCGGTTGCAGGCAAGAAGCCTGTGGTAG570.2805670407560544No Hit
GTGTTAAGAAGCAGAAGAAGCCTCTGGTGGGAAAAAAGGCAGCAGCTACC540.2658003544004725No Hit
TTATGGAATAATCAAATTTAAGAGTTTATGCAGCAGGCTTCTTCTCCTCT480.23626698168930893No Hit
CTGTATAATCAGGTCTTTATTCAAAAGAAGCTGTCCAAAATGATTTGACC460.22642252411892105No Hit
CTCACTGCCTGTATAATCAGGTCTTTATTCAAAAGAAGCTGTCCAAAATG450.22150029533372714No Hit
GTGGTAGGTAAGAAAGGAAAGAAGGCTGCTGTTGGTGTTAAGAAGCAGAA430.21165583776333927No Hit
GATTTGACCTTTATGGAATAATCAAATTTAAGAGTTTATGCAGCAGGCTT420.2067336089781453No Hit
GGCAAGAAGCCTGTGGTAGGTAAGAAAGGAAAGAAGGCTGCTGTTGGTGT420.2067336089781453No Hit
GGCTTCTTCTCCTCTGTAGTAGGTTTCTTCTCTGCAGGCTTCTTTTCAGG400.19688915140775742No Hit
ATTTAAGAGTTTATGCAGCAGGCTTCTTCTCCTCTGTAGTAGGTTTCTTC360.1772002362669817No Hit
GGAAAGAAGGCTGCTGTTGGTGTTAAGAAGCAGAAGAAGCCTCTGGTGGG360.1772002362669817No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.17227800748178776No Hit
GAAGAAGCCTCTGGTGGGAAAAAAGGCAGCAGCTACCAAGAAACCAGCCC350.17227800748178776No Hit
AGTTTATGCAGCAGGCTTCTTCTCCTCTGTAGTAGGTTTCTTCTCTGCAG340.16735577869659382No Hit
CAAGAAGCCTGTGGTAGGTAAGAAAGGAAAGAAGGCTGCTGTTGGTGTTA340.16735577869659382No Hit
GGTCTTTATTCAAAAGAAGCTGTCCAAAATGATTTGACCTTTATGGAATA330.16243354991139988No Hit
CCTTTATGGAATAATCAAATTTAAGAGTTTATGCAGCAGGCTTCTTCTCC310.152589092341012No Hit
CAAAAGAAGCTGTCCAAAATGATTTGACCTTTATGGAATAATCAAATTTA310.152589092341012No Hit
GCCTGTGGTAGGTAAGAAAGGAAAGAAGGCTGCTGTTGGTGTTAAGAAGC280.1378224059854302No Hit
CCTCTGTAGTAGGTTTCTTCTCTGCAGGCTTCTTTTCAGGGGCTGGTTTC270.13290017720023625No Hit
GTAAGAAAGGAAAGAAGGCTGCTGTTGGTGTTAAGAAGCAGAAGAAGCCT270.13290017720023625No Hit
TGAGAAGGCGGCGGTTGCAGGCAAGAAGCCTGTGGTAGGTAAGAAAGGAA260.12797794841504234No Hit
CTTCTCCTCTGTAGTAGGTTTCTTCTCTGCAGGCTTCTTTTCAGGGGCTG250.12305571962984839No Hit
ATTCAAAAGAAGCTGTCCAAAATGATTTGACCTTTATGGAATAATCAAAT240.11813349084465447No Hit
CTGTAGTAGGTTTCTTCTCTGCAGGCTTCTTTTCAGGGGCTGGTTTCTTG240.11813349084465447No Hit
GGTTTCTTCTCTGCAGGCTTCTTTTCAGGGGCTGGTTTCTTGGTAGCTGC240.11813349084465447No Hit
AATCAGGTCTTTATTCAAAAGAAGCTGTCCAAAATGATTTGACCTTTATG240.11813349084465447No Hit
TGTATAATCAGGTCTTTATTCAAAAGAAGCTGTCCAAAATGATTTGACCT240.11813349084465447No Hit
GGTTTCTTGGTAGCTGCTGCCTTTTTTCCCACCAGAGGCTTCTTCTGCTT230.11321126205946053No Hit
ACCTACTACAGAGGAGAAGAAGCCTGCTGCATAAACTCTTAAATTTGATT220.10828903327426659No Hit
GAAAGAAGGCTGCTGTTGGTGTTAAGAAGCAGAAGAAGCCTCTGGTGGGA220.10828903327426659No Hit
AGCCTGTGGTAGGTAAGAAAGGAAAGAAGGCTGCTGTTGGTGTTAAGAAG220.10828903327426659No Hit
GCCTGTATAATCAGGTCTTTATTCAAAAGAAGCTGTCCAAAATGATTTGA210.10336680448907265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)