FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61722
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT44727.245390622468488No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT15762.5533845306373744TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT11371.8421308447555167RNA PCR Primer, Index 1 (95% over 23bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG10201.6525712063769806TruSeq Adapter, Index 1 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGAACTCGTATGCCGT5460.8846116457665014No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT4990.8084637568452091TruSeq Adapter, Index 1 (96% over 26bp)
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG3150.510352872557597TruSeq Adapter, Index 1 (95% over 24bp)
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA3090.5006318654612618RNA PCR Primer, Index 1 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.48605035481675896No Hit
GTACAGGTAAGATATACTGGAGTCACAGAGCAATATGCATTAACAGGATA2730.4423058228832507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.4342049836363047No Hit
GTATAAATCTGTCTGTTTAGAATCTGTGCTGTGTAAGGGCATTCGTACTC2390.387220116004018No Hit
GATGTATGGTGGAAAAAACTGTATCTAACACTGAAACTACTACAGGACTC2280.36939826966073686No Hit
GTACAGTAGTTTTTTATCACTAAAAGTTGGACTCATTGATGGAGTCCTGT2240.36291759826318004No Hit
GTGTAAGGGCATTCGTACTCATGCTGTTACTGTACTTATGCACCATTCAG2120.3434755840705097No Hit
GGATACAACAGTTCATAAAAACTGAGTAACTATGCACACAAATTTCTTAA2060.3337545769741745No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGAGCTCGTATGCCGT1820.2948705485888338No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC1770.28676970934188784No Hit
AATTAAGACTATGCAGATTTCATATATTTCTGAATCAAAAACACCTTTGT1760.2851495414924986No Hit
TGCATACACAGCTCAATGGGTCACTTGGAACAAACTTGCTTGACTATATT1750.28352937364310943No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGAACTCGTATGCCGTCTT1600.25922685590227146No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC1540.2495058488059363Illumina PCR Primer Index 5 (95% over 24bp)
GGTCAGTACAGGTAAGATATACTGGAGTCACAGAGCAATATGCATTAACA1490.24140500955899033No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.23654450601082272No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT1430.23168400246265514No Hit
CTACTGTACTGGGCAAACTTGGCAGTCATAAACCCACATCTACTCTAACA1420.23006383461326593No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT1410.22844366676387676No Hit
GGGTAGAGGAGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT1390.22520333106509832No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG1360.22034282751693074No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACATC1290.20900165257120637No Hit
GCTCTAGAGGGGGTAGAGGAGGTGCTATAGGGTAAATACGGGCCCTATTT1290.20900165257120637No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGAACTCGTATGCCGTCTTCT1280.20738148472181717RNA PCR Primer, Index 5 (95% over 21bp)
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT1250.20252098117364958No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT1240.2009008133242604No Hit
CTTATGCACCATTCAGACTTGTTAGAGTAGATGTGGGTTTATGACTGCCA1220.19766047762548197No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC1190.19279997407731442No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGACCTCGTATGCCGT1140.18469913483036843No Hit
CCATACATCTGTGCATTTTCTCTTTAGGTGACTGTTTAAGAAATTTGTGT1100.17821846343281164No Hit
GTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCG1100.17821846343281164No Hit
GTATGAAACTGTGGTTTGCTCCACAGATTTCAGAGCATTGACCGTAGTAT1080.17497812773403326No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTCTTTTCTTTTTTTTTTTTTTTT1070.17335795988464403No Hit
CTCCTATATTTACCGGTGTCAGGCATTGTTCTCAGTCTGTACTTGTATTA1040.16849745633647648No Hit
GTACATGGGGAGCCAGAGAGGAATGAGAAGAATAAGTCATTTTTAAGAAC1040.16849745633647648No Hit
ATTCAGACTTGTTAGAGTAGATGTGGGTTTATGACTGCCAAGTTTGCCCA1020.16525712063769807No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG1020.16525712063769807No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC1000.16201678493891966No Hit
TCTATGACCAGGCCTTAAGGGAAGGTCAGTTTTTTAAAAAAACCAAGTAG990.16039661708953046No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA990.16039661708953046No Hit
TGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATAC980.15877644924014128No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA980.15877644924014128No Hit
TTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTG960.15553611354136287No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC960.15553611354136287No Hit
CATTGATGGAGTCCTGTAGTAGTTTCAGTGTTAGATACAGTTTTTTCCAC900.1458151064450277No Hit
GTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCT870.14095460289686013No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC870.14095460289686013No Hit
GATATACTGGAGTCACAGAGCAATATGCATTAACAGGATACAACAGTTCA860.13933443504747092No Hit
CCATCAATGAGTCCAACTTTTAGTGATAAAAAACTACTGTACTGGGCAAA850.13771426719808172No Hit
ATGTAAAGGATGCGTAGGGATGGGAGGGCGATGAGGACTAGGATGATGGC850.13771426719808172No Hit
GGTAAGATATACTGGAGTCACAGAGCAATATGCATTAACAGGATACAACA840.13609409934869252No Hit
CTATATTACTGAGCAGAAACAAAACGGAAGCACAAACACCTTTCTTTTTT840.13609409934869252No Hit
TCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAG800.12961342795113573No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGAGCTCGTATGCCGTCTT800.12961342795113573No Hit
GTACATGGGGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGAGGGTGAGGA790.12799326010174655No Hit
TGTTAGAGTAGATGTGGGTTTATGACTGCCAAGTTTGCCCAGTACAGTAG780.12637309225235735No Hit
CTACAGGACTCCATCAATGAGTCCAACTTTTAGTGATAAAAAACTACTGT780.12637309225235735No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAGGCAT770.12475292440296815No Hit
GTTTAGAATCTGTGCTGTGTAAGGGCATTCGTACTCATGCTGTTACTGTA760.12313275655357896No Hit
TCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTAC750.12151258870418974No Hit
GTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTT740.11989242085480055No Hit
GTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTA730.11827225300541136No Hit
ACACAATGATGTCTGTATAAATCTGTCTGTTTAGAATCTGTGCTGTGTAA730.11827225300541136No Hit
GTTACTGTACTTATGCACCATTCAGACTTGTTAGAGTAGATGTGGGTTTA730.11827225300541136No Hit
GAGTTAATGTGAAATTGTAAATGGAAATTTTTCCTTAAAATACAACACAA720.11665208515602217No Hit
GTCCTGTAGTAGTTTCAGTGTTAGATACAGTTTTTTCCACCATACATCTG720.11665208515602217No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC720.11665208515602217No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATATCGTATGCCGT720.11665208515602217No Hit
ATACAACACAATGATGTCTGTATAAATCTGTCTGTTTAGAATCTGTGCTG710.11503191730663297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTCTTTTCTTTTTTTTTTTTTTT710.11503191730663297No Hit
TCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCA710.11503191730663297No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACATCCCCTATCATAG700.11341174945724378No Hit
CTTTTATGGAATCCCCCTTTTTTCATTTTCATTTTTTTTTTGCATAGGGA700.11341174945724378No Hit
GTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCT700.11341174945724378No Hit
CAACAAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACC690.11179158160785459No Hit
GGGTATGAAACTGTGGTTTGCTCCACAGATTTCAGAGCATTGACCGTAGT690.11179158160785459No Hit
GTGCTGTGTAAGGGCATTCGTACTCATGCTGTTACTGTACTTATGCACCA670.10855124590907618No Hit
GCAATATGCATTAACAGGATACAACAGTTCATAAAAACTGAGTAACTATG670.10855124590907618No Hit
TCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC670.10855124590907618No Hit
GTTCAGGACATATGAGCTCTGACTCTGTTTAAGATCACAATCAGGCCAGG660.10693107805968699No Hit
GCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAAT660.10693107805968699No Hit
GATTTATACAGACATCATTGTGTTGTATTTTAAGGAAAAATTTCCATTTA650.1053109102102978No Hit
GAGTAGATGTGGGTTTATGACTGCCAAGTTTGCCCAGTACAGTAGTTTTT650.1053109102102978No Hit
GTTATGTAAAGGATGCGTAGGGATGGGAGGGCGATGAGGACTAGGATGAT650.1053109102102978No Hit
CTTCAATACTTCTGCCGCTTTGGAAATTGTAACAGTTAATTACTTTGAAT650.1053109102102978No Hit
GAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTA640.10369074236090858No Hit
GGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCCTAATGTGGGGACA640.10369074236090858No Hit
CAGTTAATACTTAATGGAATATTCCAGTAGTTCAGGACATATGAGCTCTG630.1020705745115194No Hit
TCTCCGAGCCCACGAGACTAGGCATGAACTCGTATGCCGTCTTCTGCTTG630.1020705745115194TruSeq Adapter, Index 5 (96% over 26bp)
GTGTACTCGTAGGTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAG630.1020705745115194No Hit
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC620.1004504066621302No Hit
GTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAAT620.1004504066621302No Hit
GGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGA620.1004504066621302No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)