FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61722
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGGTAAGATATACTGGAGTCACAGAGCAATATGCATTAACAGGATA2740.4439259907326399No Hit
GTACAGTAGTTTTTTATCACTAAAAGTTGGACTCATTGATGGAGTCCTGT2680.4342049836363047No Hit
GTATAAATCTGTCTGTTTAGAATCTGTGCTGTGTAAGGGCATTCGTACTC2630.4261041443893587No Hit
GATGTATGGTGGAAAAAACTGTATCTAACACTGAAACTACTACAGGACTC2390.387220116004018No Hit
GTGTAAGGGCATTCGTACTCATGCTGTTACTGTACTTATGCACCATTCAG2310.37425877320890444No Hit
TGCATACACAGCTCAATGGGTCACTTGGAACAAACTTGCTTGACTATATT1990.32241340202845015No Hit
GGATACAACAGTTCATAAAAACTGAGTAACTATGCACACAAATTTCTTAA1940.31431256278150416No Hit
AATTAAGACTATGCAGATTTCATATATTTCTGAATCAAAAACACCTTTGT1870.30297138783577976No Hit
CCATACATCTGTGCATTTTCTCTTTAGGTGACTGTTTAAGAAATTTGTGT1720.27866887009494185No Hit
GCTCTAGAGGGGGTAGAGGAGGTGCTATAGGGTAAATACGGGCCCTATTT1520.2462655131071579No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT1490.24140500955899033No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACATC1450.23492433816143352No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG1450.23492433816143352No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC1430.23168400246265514No Hit
CTACTGTACTGGGCAAACTTGGCAGTCATAAACCCACATCTACTCTAACA1410.22844366676387676No Hit
CTCCTATATTTACCGGTGTCAGGCATTGTTCTCAGTCTGTACTTGTATTA1410.22844366676387676No Hit
GGTCAGTACAGGTAAGATATACTGGAGTCACAGAGCAATATGCATTAACA1400.22682349891448755No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG1380.22358316321570917No Hit
GTACATGGGGAGCCAGAGAGGAATGAGAAGAATAAGTCATTTTTAAGAAC1340.21710249181815236No Hit
GGGTAGAGGAGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT1280.20738148472181717No Hit
CTTATGCACCATTCAGACTTGTTAGAGTAGATGTGGGTTTATGACTGCCA1270.205761316872428No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT1250.20252098117364958No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT1220.19766047762548197No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC1200.1944201419267036No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA1170.189559638378536No Hit
GTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCG1170.189559638378536No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA1120.18145879913159002No Hit
TCTATGACCAGGCCTTAAGGGAAGGTCAGTTTTTTAAAAAAACCAAGTAG1110.17983863128220084No Hit
TGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATAC1100.17821846343281164No Hit
GTACATGGGGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGAGGGTGAGGA1070.17335795988464403No Hit
GTATGAAACTGTGGTTTGCTCCACAGATTTCAGAGCATTGACCGTAGTAT1040.16849745633647648No Hit
GATATACTGGAGTCACAGAGCAATATGCATTAACAGGATACAACAGTTCA1000.16201678493891966No Hit
GTTTAGAATCTGTGCTGTGTAAGGGCATTCGTACTCATGCTGTTACTGTA880.1425747707462493No Hit
TTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTG870.14095460289686013No Hit
TCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAG860.13933443504747092No Hit
ATTCAGACTTGTTAGAGTAGATGTGGGTTTATGACTGCCAAGTTTGCCCA850.13771426719808172No Hit
ACACAATGATGTCTGTATAAATCTGTCTGTTTAGAATCTGTGCTGTGTAA850.13771426719808172No Hit
CTACAGGACTCCATCAATGAGTCCAACTTTTAGTGATAAAAAACTACTGT850.13771426719808172No Hit
GAGTTAATGTGAAATTGTAAATGGAAATTTTTCCTTAAAATACAACACAA830.13447393149930334No Hit
CCTAAAGAGAAAATGCACAGATGTATGGTGGAAAAAACTGTATCTAACAC830.13447393149930334No Hit
CATTGATGGAGTCCTGTAGTAGTTTCAGTGTTAGATACAGTTTTTTCCAC820.13285376364991414No Hit
CTATATTACTGAGCAGAAACAAAACGGAAGCACAAACACCTTTCTTTTTT810.13123359580052493No Hit
TCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTAC810.13123359580052493No Hit
CTTCAATACTTCTGCCGCTTTGGAAATTGTAACAGTTAATTACTTTGAAT800.12961342795113573No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC800.12961342795113573No Hit
CCATCAATGAGTCCAACTTTTAGTGATAAAAAACTACTGTACTGGGCAAA790.12799326010174655No Hit
GTGCTGTGTAAGGGCATTCGTACTCATGCTGTTACTGTACTTATGCACCA780.12637309225235735No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACATCCCCTATCATAG780.12637309225235735No Hit
GTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTA770.12475292440296815No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC770.12475292440296815No Hit
GCAATATGCATTAACAGGATACAACAGTTCATAAAAACTGAGTAACTATG770.12475292440296815No Hit
AACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTA760.12313275655357896No Hit
GTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGCTTCAATCG750.12151258870418974No Hit
CATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCA750.12151258870418974No Hit
ATGTAAAGGATGCGTAGGGATGGGAGGGCGATGAGGACTAGGATGATGGC740.11989242085480055No Hit
GGTAAGATATACTGGAGTCACAGAGCAATATGCATTAACAGGATACAACA730.11827225300541136No Hit
GATTTATACAGACATCATTGTGTTGTATTTTAAGGAAAAATTTCCATTTA720.11665208515602217No Hit
ATATTACTGAGCAGAAACAAAACGGAAGCACAAACACCTTTCTTTTTTTG720.11665208515602217No Hit
GGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCCTAATGTGGGGACA720.11665208515602217No Hit
GTCCTGTAGTAGTTTCAGTGTTAGATACAGTTTTTTCCACCATACATCTG720.11665208515602217No Hit
TTATTATACGAATGGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACG710.11503191730663297No Hit
GTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAAT700.11341174945724378No Hit
CTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCT700.11341174945724378No Hit
GTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTT700.11341174945724378No Hit
GTATTAACTGAGAGGGAGGATTGCTGTAGGGATTAAGCGATTTAATACAA690.11179158160785459No Hit
GTACTGGGCAAACTTGGCAGTCATAAACCCACATCTACTCTAACAAGTCT690.11179158160785459No Hit
GTATCAACGCAGAGTACATGGGATGGCACATGCAGCGCAAGTAGGTCTAC690.11179158160785459No Hit
GCTCAATGGGTCACTTGGAACAAACTTGCTTGACTATATTACTGAGCAGA690.11179158160785459No Hit
GTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCT670.10855124590907618No Hit
GAGTAACTATGCACACAAATTTCTTAAACAGTCACCTAAAGAGAAAATGC670.10855124590907618No Hit
GTACATGGGAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT670.10855124590907618No Hit
CCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTC670.10855124590907618No Hit
ATACAACAGTTCATAAAAACTGAGTAACTATGCACACAAATTTCTTAAAC670.10855124590907618No Hit
TCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCA670.10855124590907618No Hit
GCCCAGTACAGTAGTTTTTTATCACTAAAAGTTGGACTCATTGATGGAGT660.10693107805968699No Hit
TCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC660.10693107805968699No Hit
GAGTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACA660.10693107805968699No Hit
ATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTTCA650.1053109102102978No Hit
GTACATGGGATGGCAGGATTGTACTATAAGCAAATGAATTAAACAGCTAT650.1053109102102978No Hit
GTTCAGGACATATGAGCTCTGACTCTGTTTAAGATCACAATCAGGCCAGG640.10369074236090858No Hit
CCCTTACACAGCACAGATTCTAAACAGACAGATTTATACAGACATCATTG640.10369074236090858No Hit
GTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCT630.1020705745115194No Hit
ATATTTACCGGTGTCAGGCATTGTTCTCAGTCTGTACTTGTATTAAATCG630.1020705745115194No Hit
GTCTTGTTTTTTGTAGAGATGGGGTTTCACCATGGTGGCTGGGCTGGTCT630.1020705745115194No Hit
GTCTGTACTTGTATTAAATCGCTTAATCCCTACAGCAATCCTCCCTCTCA620.1004504066621302No Hit
GAGTACATGGGGAGCCAGAGAGGAATGAGAAGAATAAGTCATTTTTAAGA620.1004504066621302No Hit
CAACAAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACC620.1004504066621302No Hit
GGGTATGAAACTGTGGTTTGCTCCACAGATTTCAGAGCATTGACCGTAGT620.1004504066621302No Hit
CCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCA620.1004504066621302No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)