FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23502
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT586524.955322951238195No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG21148.994979150710577RNA PCR Primer, Index 39 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT20318.641817717641052RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT14456.1484129010297RNA PCR Primer, Index 39 (96% over 25bp)
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA7543.2082375968002723RNA PCR Primer, Index 39 (96% over 30bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT5792.4636201174368138RNA PCR Primer, Index 39 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG3521.497744872776785Illumina PCR Primer Index 11 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACAACTCGTATGCCGT2911.238192494255808No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC2090.8892860182112161No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT1800.7658922644881286RNA PCR Primer, Index 39 (95% over 22bp)
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGC1760.7488724363883925Illumina PCR Primer Index 11 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACAGCTCGTATGCCGT1400.5956939834907667No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC1390.5914390264658327No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC1110.4723002297676793RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACACCTCGTATGCCGT1080.4595353586928772No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACAACTCGTATGCCGTCTT950.40422091736873456TruSeq Adapter, Index 11 (95% over 21bp)
ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTT930.3957110033188665Illumina PCR Primer Index 11 (96% over 28bp)
TCTCCGAGCCCACGAGACCTCTCTACAACTCGTATGCCGTCTTCTGCTTG860.36592630414432814TruSeq Adapter, Index 11 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.3361416049697898No Hit
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAA770.3276316909199217Illumina PCR Primer Index 11 (96% over 29bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCTCTCTA750.3191217768700536No Hit
CTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC690.2935920347204493No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.2850821206705812No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACAACTCGTATGCCGTCTTCT590.25104246447110884RNA PCR Primer, Index 11 (95% over 23bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCTCTCTA510.21700280827163645No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCTCTCT510.21700280827163645No Hit
TCTCCGAGCCCACGAGACCTCTCTACAGCTCGTATGCCGTCTTCTGCTTG420.17870819504723004TruSeq Adapter, Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACAGCTCGTATGCCGTCTT370.15743340992255977TruSeq Adapter, Index 11 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.15317845289762574No Hit
CTCCGAGCCCACGAGACCTCTCTACAACTCGTATGCCGTCTTCTGCTTGA330.1404135818228236RNA PCR Primer, Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCCT330.1404135818228236TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATATCGTATGCCGT300.12764871074802145No Hit
CATCTCCGAGCCCACGAGACCTCTCTACAACTCGTATGCCGTCTTCTGCT260.11062888264828526TruSeq Adapter, Index 11 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACAGCTCGTATGCCGTCTTCT260.11062888264828526RNA PCR Primer, Index 11 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGATACCTCTCTACATCTCGTATGCCGT250.1063739256233512No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACACCTCGTATGCCGTCTT240.10211896859841717TruSeq Adapter, Index 11 (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)