FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001420991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221900
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT39981.8017124831004958No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG13720.6182965299684542RNA PCR Primer, Index 23 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT13600.6128886885984678RNA PCR Primer, Index 23 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT9520.4290220820189274RNA PCR Primer, Index 23 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.31590806669671023No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGT6060.27309598918431727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5780.26047769265434884No Hit
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA5190.23388913925191526RNA PCR Primer, Index 23 (96% over 30bp)
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT3880.17485353762956285RNA PCR Primer, Index 23 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.12618296529968456No Hit
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG2780.12528165840468677Illumina PCR Primer Index 1 (95% over 23bp)
GTGCTGAACCGCGCACGCCCAGATGCCGAGAAAAAAGAAATGAAAACAAT2730.12302839116719244No Hit
GTTCAGCACCTTGAGGAAGTCAGACGTTTTCGCCCGCATACGTGTGTGAA2610.11762054979720594No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGT2360.10635421360973411No Hit
GTCTAAGATTGGTCATACATTCCCAATTTACTCTCAGTTCCAGTCAAGCT2330.1050022532672375No Hit
ACTTTATACTGTCCAGCTCTGTTGATTTTAAAGCTTCTTCATCCAGGTCA2270.10229833258224424No Hit
GCCCCACAGGTCCTCTTTAGCCACAGGGAACCTCCTCTGGAGCTGAAAGA2240.10094637223974764No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)