FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001420993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221687
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT70673.187827883457307No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT23061.0402053345482596RNA PCR Primer, Index 31 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT15740.7100100592276498RNA PCR Primer, Index 31 (95% over 24bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG8960.40417345175856045RNA PCR Primer, Index 31 (96% over 29bp)
CTATAAGAGGTGTGGGTGTCTGTTTGGTCAGGATGTTAGAAAGTGCTGAT7880.3554561160555197No Hit
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT6770.30538552102739447RNA PCR Primer, Index 31 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6090.2747116429921466No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGT5750.2593747039745226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5390.24313559207350907No Hit
ACCTAATTGCATTCAAAACCATTTTCAAATCCATCTTTAAACTAGTCAGA5330.2404290734233401No Hit
ACTTAACACTGAACAGATAAGACCTCTTAAAAGGCAGCTGACTATATCAT4990.22509213440571615No Hit
GATAAGACCTCTTAAAAGGCAGCTGACTATATCATGTCACCATCATAGCC4390.19802694790402686No Hit
TCTTTAAACTAGTCAGAAAACAGGTTATTATTTTTTTAAATCACTTAACA4180.18855413262843557No Hit
GACTATATCATGTCACCATCATAGCCAATACAACATTTTTGCCATACTTC4130.1862987004199615No Hit
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG4020.18133674956131843RNA PCR Primer, Index 38 (95% over 24bp)
GTGTATGCGAAAAGGTTTTTAGGAAGTATGGCAAAAATGTTGTATTGGCT3950.17817914446945468No Hit
ACACTGAACAGATAAGACCTCTTAAAAGGCAGCTGACTATATCATGTCAC3870.1745704529358961No Hit
CTCTATAAGAGGTGTGGGTGTCTGTTTGGTCAGGATGTTAGAAAGTGCTG3530.15923351391827217No Hit
GAACAGATAAGACCTCTTAAAAGGCAGCTGACTATATCATGTCACCATCA3420.1542715630596291No Hit
ATCATAGCCAATACAACATTTTTGCCATACTTCCTAAAAACCTTTTCGCA3240.14615200710912232No Hit
CTTAAAAGGCAGCTGACTATATCATGTCACCATCATAGCCAATACAACAT3060.1380324511586155No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTAGCTCGTATGCCGT2560.11547812907387442No Hit
GTACTCTATAAGAGGTGTGGGTGTCTGTTTGGTCAGGATGTTAGAAAGTG2530.11412486974878995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2440.11006509177353657No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)