FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001420997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128683
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT37692.9289028076746737No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT12290.9550601089499001RNA PCR Primer, Index 33 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9200.7149351507192092No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG9120.7087183233216509RNA PCR Primer, Index 33 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT9070.7048328061981769RNA PCR Primer, Index 33 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7770.6038093609878539No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGT4710.36601571303124736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.3077329561791379No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT3650.28364275001359934RNA PCR Primer, Index 33 (96% over 27bp)
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA2760.2144805452157628No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA2470.1919445458996138RNA PCR Primer, Index 33 (96% over 29bp)
GTGTAAGCTAGTCATATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTG2350.18261930480327626No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG2260.17562537398102313RNA PCR Primer, Index 15 (95% over 24bp)
CCCTTAGCCCTGCCCTCTCACCAGAGCAAAATTCACTGGGGACTTTTCCC2070.16086040891182207No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT2060.16008330548712726No Hit
GTCCAATACAGTCAATAAGAAAACTAGTTGTGAACAACAGGTAAAAAAAG2000.15542068493895853No Hit
AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT1900.1476496506920106No Hit
TTACTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGTCGCAT1840.14298703014384184No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1790.13910151302036788No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGT1680.13055337534872516No Hit
GTACATGGGGGCAGATAATTTTTAGCTTAGGGCCTGGTGGCTAGGTCGGT1670.12977627192403035No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTGAGTAGGAGAGTGATAT1660.12899916849933557No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTT1610.1251136513758616No Hit
CTCCAATAGAGATTACAATAGAGATCTCCAACATGATTTCATGCATTTAG1610.1251136513758616No Hit
GTACATGGGATTTAAGGAAAAAAAATAAAATAAAACATTTTGGATTTTCA1570.12200523767708245No Hit
GAACAAACATGGGGAAGAGGGCAAAAAGCATTTAGCACAAAATCTCATTA1570.12200523767708245No Hit
TCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGGCAAGCCAA1550.12045103082769285No Hit
GTACATGGGAATCTTCTCAAATATCTGATGTAACTACCAGAAAGTCCCTT1520.11811972055360848No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.1173426171289137No Hit
TTACAATAGAGATCTCCAACATGATTTCATGCATTTAGAGGAGAAATATT1510.1173426171289137No Hit
CTATAGGGGAAGGCAAAGTATGCTGGCTGGCTATAGGAAGTGACACCATA1490.11578841027952409No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT1480.1150113068548293No Hit
CTCTTCAATCAGCCACATAGCCCTCGTAGTAACAGCCATTCTCATCCAAA1480.1150113068548293No Hit
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCAT1410.10957158288196575No Hit
GTATTGGACTGTCTCTTCTTGTAGAGACTGATTTTGGCCAACATATTAAT1400.10879447945727097No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.1072402726078814Clontech SMART CDS Primer II A (100% over 21bp)
GCGTTATAGTGAAACATTTCATTTAGAAATCTGGACGTTCCTTCTTCAGC1380.1072402726078814No Hit
CTTCTAGCAAGCCTCGCTAACCTCGCCTTACCCCCCACTATTAACCTACT1360.10568606575849179No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC1340.10413185890910222No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC1340.10413185890910222RNA PCR Primer, Index 15 (96% over 25bp)
CAATAGTACCGATCAGTTAACTCAGCGCTGAAGGGCTTGTTTTATGAAAG1320.10257765205971263No Hit
TTCTAAAGCAGCGTTATAGTGAAACATTTCATTTAGAAATCTGGACGTTC1320.10257765205971263No Hit
CCTGATAGTGTATGAAACACTCTTTAAAATTCAACTGCTTCCCCATCAGG1320.10257765205971263No Hit
GTATCAACGCAGAGTACATGGGATTTAAGGAAAAAAAATAAAATAAAACA1310.10180054863501785No Hit
ATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAGCCACAGA1310.10180054863501785No Hit
CTTTCATATAGTACCAATTAACATTAGCCTAATGAAACTAAGTCAAATAT1300.10102344521032303No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA1300.10102344521032303No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)