FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001420998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128683
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4710.36601571303124736No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA2790.21681185548984716No Hit
GTGTAAGCTAGTCATATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTG2490.19349875274900336No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT2360.18339640822797107No Hit
CCCTTAGCCCTGCCCTCTCACCAGAGCAAAATTCACTGGGGACTTTTCCC2310.17951089110449708No Hit
GTCCAATACAGTCAATAAGAAAACTAGTTGTGAACAACAGGTAAAAAAAG2070.16086040891182207No Hit
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCAT2050.15930620206243248No Hit
AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT1940.15075806439078976No Hit
GAACAAACATGGGGAAGAGGGCAAAAAGCATTTAGCACAAAATCTCATTA1930.14998096096609498No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTGAGTAGGAGAGTGATAT1900.1476496506920106No Hit
GTACATGGGGGCAGATAATTTTTAGCTTAGGGCCTGGTGGCTAGGTCGGT1740.1352159958968939No Hit
CTCCAATAGAGATTACAATAGAGATCTCCAACATGATTTCATGCATTTAG1730.13443889247219912No Hit
TTACAATAGAGATCTCCAACATGATTTCATGCATTTAGAGGAGAAATATT1700.13210758219811475No Hit
GTACATGGGAATCTTCTCAAATATCTGATGTAACTACCAGAAAGTCCCTT1630.1266678582252512No Hit
CCTGATAGTGTATGAAACACTCTTTAAAATTCAACTGCTTCCCCATCAGG1630.1266678582252512No Hit
GTACATGGGATTTAAGGAAAAAAAATAAAATAAAACATTTTGGATTTTCA1620.1258907548005564No Hit
TTCTAAAGCAGCGTTATAGTGAAACATTTCATTTAGAAATCTGGACGTTC1600.12433654795116682No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC1600.12433654795116682No Hit
TCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGGCAAGCCAA1590.12355944452647202No Hit
CTATAGGGGAAGGCAAAGTATGCTGGCTGGCTATAGGAAGTGACACCATA1590.12355944452647202No Hit
TTACTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGTCGCAT1530.11889682397830327No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT1520.11811972055360848No Hit
GTATCAACGCAGAGTACATGGGATTTAAGGAAAAAAAATAAAATAAAACA1460.11345710000543972No Hit
GTATCAACGCAGAGTACTTTTTAATTTTTTTTTATTTTTATTTTTTTGTA1430.11112578973135534No Hit
GAATAGTAATGAGGATGTAAGTCCGTGGGCGATTATGAGAATGACTGCGC1430.11112578973135534No Hit
GAGTTAAGTATGTAATTATAAGTCCAGTAACAATTTCTACAAAAATGCAC1420.11034868630666055No Hit
GCATTATGTGGTGTAATCAAACCCGATGCTTTCAGATGACCTACTTACAT1360.10568606575849179No Hit
GTATTGTAATGTGAGAAGGTGGTAGCACTAATGGTTGAATACAAGAGTTA1360.10568606575849179No Hit
GTATTGGACTGTCTCTTCTTGTAGAGACTGATTTTGGCCAACATATTAAT1360.10568606575849179No Hit
TGCTAGAAGTCATCAAAAAGCTATTAGTGGGAGTAGAGTTTGAAGTCCTT1350.104908962333797No Hit
TCTCTAGATTTATCTCTGACTAATGCAGGAATCCCCTTTCAAATCAGGCA1340.10413185890910222No Hit
CAATAGAGATCTCCAACATGATTTCATGCATTTAGAGGAGAAATATTTCC1340.10413185890910222No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA1310.10180054863501785No Hit
ATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAGCCACAGA1300.10102344521032303No Hit
CTCTTCAATCAGCCACATAGCCCTCGTAGTAACAGCCATTCTCATCCAAA1290.10024634178562825No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)