FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001420999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001420999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23447
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT528022.5188723504073No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT19048.120441847571119TruSeq Adapter, Index 3 (95% over 21bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG15636.66609800827398TruSeq Adapter, Index 3 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT13355.693692156779119RNA PCR Primer, Index 3 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGT7533.211498272700132No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT5902.51631338764021TruSeq Adapter, Index 3 (96% over 26bp)
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA5172.2049729176440485RNA PCR Primer, Index 3 (96% over 28bp)
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG3431.462873715187444TruSeq Adapter, Index 3 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGT3061.3050710112167867No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTT2581.1003539898494477No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC2090.8913720305369556No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCT1780.7591589542372159Illumina PCR Primer Index 8 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT1570.6695952573890049No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC1560.6653303194438521Illumina PCR Primer Index 12 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAACCTCGTATGCCGT1540.6568004435535463No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC1430.6098861261568644No Hit
TCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCTTG1190.5075276154731949Illumina PCR Primer Index 8 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTT1090.4648782360216659No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAGG870.3710496012283021No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.36678466328314924No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.3539898494476905No Hit
CTTTACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC770.32840022177677314No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATATCGTATGCCGT670.28575084232524417No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTTCT670.28575084232524417Illumina PCR Primer Index 8 (95% over 21bp)
ATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTT660.2814859043800913TruSeq Adapter, Index 3 (96% over 27bp)
CTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC610.2601612146543268No Hit
TCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTTCTGCTTG610.2601612146543268Illumina PCR Primer Index 8 (96% over 26bp)
CTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCTTGA520.2217767731479507Illumina PCR Primer Index 8 (96% over 26bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACGTAGAGG510.21751183520279782No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCT460.1961871454770333Illumina PCR Primer Index 8 (95% over 24bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAG460.1961871454770333No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.18765726958672752No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAACCTCGTATGCCGTCTTCT320.13647801424489273Illumina PCR Primer Index 8 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTC310.13221307629973986No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCCT300.12794813835458693No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTTCTGCT290.12368320040943405Illumina PCR Primer Index 8 (95% over 24bp)
CACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTG280.11941826246428114Illumina PCR Primer Index 8 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATATCGTATGCCGTCTT280.11941826246428114No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAACCTCGTATGCCGTCTT280.11941826246428114No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAAATCGTATGCCGT280.11941826246428114No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG280.11941826246428114RNA PCR Primer, Index 31 (96% over 29bp)
CTTATACACATCTCAGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT270.11515332451912824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)