FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001421001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419123
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT178051.2546481171822315No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT70940.4998861973204578TruSeq Adapter, Index 11 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45830.3229459321003183No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT43950.3096983136768272RNA PCR Primer, Index 11 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35570.25064775921466986No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22600.15925328530366994No Hit
TTCTTACACCGCGAGGAAGCTTCCTCGCAACTTTGTGGTAGATTACTATG21670.15269994214736848No Hit
CTCCTGGACCCAGGACTCACTGGGGTCAGCGCAGACTTGCTTGCCTCTTT21020.14811964854350188No Hit
TCTCTGAGCAGCTCAGTTCAGTTCCAGGTCATACACGTACTCCTGGACCC18270.1287414832963739No Hit
GTCCAGGAGTACGTGTATGACCTGGAACTGAACTGAGCTGCTCAGAGACA18110.12761402640926828No Hit
TCCCTGAAGACTTCCTGTCTCTGAGCAGCTCAGTTCAGTTCCAGGTCATA17870.12592284107860982No Hit
TCCCCATAGGGGACACTTATCCTTTGGCTAAACTAATATAAATAATGGAA14980.10555815105526441No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)