FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89150
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT1222113.708356702187324No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT51125.7341559169938305RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT30823.457094784071789RNA PCR Primer, Index 33 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9071.0173864273696018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7970.8939988782950085No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG7540.8457655636567583RNA PCR Primer, Index 15 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5780.6483454851374089No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC5490.6158160403813797No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT4970.5574873808188446RNA PCR Primer, Index 33 (95% over 21bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG3170.3555804823331464RNA PCR Primer, Index 33 (96% over 29bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT2880.32305103757711723RNA PCR Primer, Index 33 (96% over 27bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.30173864273696016Clontech SMART CDS Primer II A (100% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC2150.2411665731912507No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGC2110.23667975322490187No Hit
CTTTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC2010.22546270330902973No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG1810.2030286034772855TruSeq Adapter, Index 3 (96% over 28bp)
CTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC1440.16152551878855861No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA1360.1525518788558609No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG1290.1446999439147504TruSeq Adapter, Index 1 (96% over 28bp)
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC1290.1446999439147504RNA PCR Primer, Index 15 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGT1140.12787436904094224No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG1140.12787436904094224RNA PCR Primer, Index 31 (96% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.12450925406618059No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG1070.12002243409983174RNA PCR Primer, Index 17 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.1155356141334829No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA950.1065619742007852RNA PCR Primer, Index 33 (96% over 29bp)
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC910.10207515423443635No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA900.10095344924284913No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)