FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237944
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT39381.6550112631543556No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT14030.5896345358571765RNA PCR Primer, Index 26 (100% over 22bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG11160.46901792018290017RNA PCR Primer, Index 26 (100% over 29bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT9610.4038765423797196RNA PCR Primer, Index 26 (100% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7060.29670846921964833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6000.2521601721413442No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT4210.17693238745250983RNA PCR Primer, Index 26 (100% over 27bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA3530.14835423460982416RNA PCR Primer, Index 26 (100% over 29bp)
GTACAAAGACTTTGTAAATGTGATTCAGGGCCCCCAGCACCCCTGTGTCT2910.12229768348855193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.11599367918501832No Hit
CTTTTACATAGCCTAATAACTCAGCAAGGCCTCAACGTCTGTGCTAATTT2680.11263154355646707No Hit
GCTTAAATCTATCTATATTTGAAAAAACGTAGTCACAAGTACCACAAATG2640.11095047574219144No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGT2620.11010994183505363No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG2530.10632753925293346RNA PCR Primer, Index 26 (96% over 27bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)