FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37143
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT31998.612659182080069No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG12033.2388336967934737RNA PCR Primer, Index 23 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT10392.7972969334733326RNA PCR Primer, Index 23 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT8002.1538378698543466RNA PCR Primer, Index 23 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGT5191.3973023180680075No Hit
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA4021.082303529601809RNA PCR Primer, Index 23 (96% over 30bp)
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT3410.9180733920254153RNA PCR Primer, Index 23 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3020.8130737958700158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2600.6999973077026627No Hit
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG1990.5357671701262687Illumina PCR Primer Index 1 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGT1890.5088441967530893No Hit
GTACATGGGCAGCAAGCTCCAGTGCTACGTGTCCCTGGCATTTTAGGTGT1680.45230595266941276No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTT1560.41999838462159755No Hit
GTAGTAGGACATCTGTTTCTCGCCATCGTGATACAGAGAACACCCGAAGC1520.4092291952723258No Hit
GTACATGGGGCATCTAGACCTAGCCGGGGCTGGCGTAGTAGTAGGACATC1400.37692162722451067No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCT1270.34192176183937756No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGACCTCGTATGCCGT1190.3203833831408341No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.3203833831408341No Hit
TTTGTATTCTGTGAGTTTCTCACACCCTCACACTTGTTCACCAATCTCTA1190.3203833831408341No Hit
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC1170.3149987884661982No Hit
ACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGC1160.31230649112888026Illumina PCR Primer Index 1 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT1110.2988450044422906RNA PCR Primer, Index 23 (95% over 22bp)
GTCCAAGACCGGTTCATTGCAGCTCATTTGCAAGTCAGAACCAAATACAG1110.2988450044422906No Hit
ATCTAGACCTAGCCGGGGCTGGCGTAGTAGTAGGACATCTGTTTCTCGCC1000.2692297337317933No Hit
GTATTTGGTTCTGACTTGCAAATGAGCTGCAATGAACCGGTCTTGGACCG970.26115284171983955No Hit
ACCAAATACAGACCAACTTGATTATGATGTTGGAGAAGAGCATCAGTCTC970.26115284171983955No Hit
GTATCAACGCAGAGTACATGGGCAGCAAGCTCCAGTGCTACGTGTCCCTG950.2557682470452037No Hit
TCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCTGCTTG940.2530759497078857Illumina Single End Adapter 1 (96% over 25bp)
CCCTCACACTTGTTCACCAATCTCTAAGATGAAAAACTCTCTTCCAAAAT880.23692216568397811No Hit
GTCATGGATCAGGTAATGCAGTTTGTTGAGCCAAGTCGGCAGTTTGTAAA870.23422986834666018No Hit
GTATCAACGCAGAGTACATGGGGCATCTAGACCTAGCCGGGGCTGGCGTA820.2207683816600705No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTT760.2046145976361629No Hit
GTACATGGGGAAGCTCCCAGAGACTGGTTTTCTTGGGATGCAGGCAGAAG750.20192230029884498No Hit
TCCTATTGCTGTTGCCATGGCAATCTTCTGGAATTCTTTTCTATCAGGTT750.20192230029884498No Hit
TTGTAAAGGACTCCATTCGGCTGGTTAAAAGATGCACTAAACCTGATAGA740.19923000296152704No Hit
GAGTACATGGGGCATCTAGACCTAGCCGGGGCTGGCGTAGTAGTAGGACA740.19923000296152704No Hit
TCTTTGAATGGTATCTCCTCTTCACTGATTAACATCTCTTCTTCCTCCTC730.1965377056242091No Hit
CCTCTTCACTGATTAACATCTCTTCTTCCTCCTCCTCTTCCTCTTCACTA730.1965377056242091No Hit
CCTGGAGACTGATGCTCTTCTCCAACATCATAATCAAGTTGGTCTGTATT720.1938454082868912No Hit
TCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTTCTGCTTG720.1938454082868912Illumina Single End Adapter 1 (100% over 25bp)
GATTTTCTCCGTGGCTTTGGGGTTTCCCTTTCTAAGTGGGGTTTGTAGGT710.19115311094957327No Hit
GAGTACATGGGCAGCAAGCTCCAGTGCTACGTGTCCCTGGCATTTTAGGT700.18846081361225533No Hit
GGTATCAACGCAGAGTACATGGGCAGCAAGCTCCAGTGCTACGTGTCCCT690.1857685162749374No Hit
TATCAACGCAGAGTACATGGGCAGCAAGCTCCAGTGCTACGTGTCCCTGG670.18038392160030153No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC630.1696147322510298No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATATCGTATGCCGT600.161537840239076No Hit
TATCAACGCAGAGTACATGGGGCATCTAGACCTAGCCGGGGCTGGCGTAG600.161537840239076No Hit
CTACTAATGCCACCTGGAGACTGATGCTCTTCTCCAACATCATAATCAAG600.161537840239076No Hit
ACGCAGAGTACATGGGGCATCTAGACCTAGCCGGGGCTGGCGTAGTAGTA600.161537840239076No Hit
CTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCTGCTTGA580.15615324556444013Illumina Single End Adapter 1 (96% over 25bp)
GCTTTGGGGTTTCCCTTTCTAAGTGGGGTTTGTAGGTCTCATCTCTTGGA580.15615324556444013No Hit
GGTAATGCAGTTTGTTGAGCCAAGTCGGCAGTTTGTAAAGGACTCCATTC570.1534609482271222No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCAGAGAG570.1534609482271222No Hit
CACTAAACCTGATAGAAAAGAATTCCAGAAGATTGCCATGGCAACAGCAA540.1453840562151684No Hit
GGTTAAAAGATGCACTAAACCTGATAGAAAAGAATTCCAGAAGATTGCCA530.14269175887785046No Hit
GTGCTACGTGTCCCTGGCATTTTAGGTGTCGGTTGGGTAGGCAGTCATGG520.13999946154053253No Hit
CTTTACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC520.13999946154053253RNA PCR Primer, Index 23 (95% over 21bp)
TCCATATTCCTATTAATAACATCATTGTTGGTGGCTGAATACATTTTGGA510.1373071642032146No Hit
CTCTTGGATCATCTTTGAATGGTATCTCCTCTTCACTGATTAACATCTCT510.1373071642032146No Hit
ACGCAGAGTACATGGGCAGCAAGCTCCAGTGCTACGTGTCCCTGGCATTT500.13461486686589666No Hit
GTTCATTGCAGCTCATTTGCAAGTCAGAACCAAATACAGACCAACTTGAT500.13461486686589666No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.13461486686589666Clontech SMART CDS Primer II A (100% over 21bp)
ATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTT490.13192256952857873Illumina PCR Primer Index 1 (96% over 26bp)
CCATTCGGCTGGTTAAAAGATGCACTAAACCTGATAGAAAAGAATTCCAG480.1292302721912608No Hit
TCTCACACCCTCACACTTGTTCACCAATCTCTAAGATGAAAAACTCTCTT480.1292302721912608No Hit
CTCCAGTGCTACGTGTCCCTGGCATTTTAGGTGTCGGTTGGGTAGGCAGT480.1292302721912608No Hit
TCCCTGATTTTCTCCGTGGCTTTGGGGTTTCCCTTTCTAAGTGGGGTTTG470.12653797485394286No Hit
TTACATAGCTGTGCCTCAGAGAAAGGGTCCTGCATTTTCTGGAATGTTCT470.12653797485394286No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTTCT470.12653797485394286No Hit
GAATATGGATCAATTTCACAAAGAAGCCAATGAATCCCATTATAGCAAAT460.12384567751662492No Hit
TCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGAA460.12384567751662492Illumina PCR Primer Index 1 (96% over 27bp)
TCGTGATACAGAGAACACCCGAAGCTCTCGGTCCAAGACCGGTTCATTGC450.12115338017930699No Hit
GTGATACAGAGAACACCCGAAGCTCTCGGTCCAAGACCGGTTCATTGCAG450.12115338017930699No Hit
GGTATCTCCTCTTCACTGATTAACATCTCTTCTTCCTCCTCCTCTTCCTC450.12115338017930699No Hit
GCTATGTAACAGGCAACCCCACCCAGGGAGAAGCCAGTACAGAGGCCCCA440.11846108284198906No Hit
GTCCTGGGGAGCCTGGGGTCACCTTGCAGAACAAGATGGTCCCAGGACTC430.11576878550467112No Hit
GATTAACATCTCTTCTTCCTCCTCCTCTTCCTCTTCACTACTAATGCCAC430.11576878550467112No Hit
GGGTTTCACCATGTTGGCCAGGCTGGTCTTGAACTCCTGTCCCTGCCCGC430.11576878550467112No Hit
GTATAATGTCCCTCAAGTTTATGGCAGTGTACCTTGTGCCACTGAATTTC420.11307648816735319No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.11307648816735319No Hit
GTCTTGGACCGAGAGCTTCGGGTGTTCTCTGTATCACGATGGCGAGAAAC420.11307648816735319No Hit
GTACTGGCTTCTCCCTGGGTGGGGTTGCCTGTTACATAGCTGTGCCTCAG420.11307648816735319No Hit
ATTAATAGGAATATGGATCAATTTCACAAAGAAGCCAATGAATCCCATTA410.11038419083003526No Hit
CTCTTCTTCCTCCTCCTCTTCCTCTTCACTACTAATGCCACCTGGAGACT400.10769189349271732No Hit
TAGGAATATGGATCAATTTCACAAAGAAGCCAATGAATCCCATTATAGCA400.10769189349271732No Hit
CTCCGTGGCTTTGGGGTTTCCCTTTCTAAGTGGGGTTTGTAGGTCTCATC400.10769189349271732No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.10769189349271732No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.10769189349271732No Hit
GCAATAGGATTTGCTATAATGGGATTCATTGGCTTCTTTGTGAAATTGAT390.10499959615539939No Hit
ACAATGATGTTATTAATAGGAATATGGATCAATTTCACAAAGAAGCCAAT390.10499959615539939No Hit
CCCCAGGACAGGGGAGAGGGATCGTCCTCATTCAGACTCTAGCTGGGGCC390.10499959615539939No Hit
CCCTGATTTTCTCCGTGGCTTTGGGGTTTCCCTTTCTAAGTGGGGTTTGT390.10499959615539939No Hit
GTATCAACGCAGAGTACATGGGCACATGGGACAGATGTAAAAGGGTCCAA390.10499959615539939No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC390.10499959615539939No Hit
AGGTAATGCAGTTTGTTGAGCCAAGTCGGCAGTTTGTAAAGGACTCCATT380.10230729881808145No Hit
ACTAATGCCACCTGGAGACTGATGCTCTTCTCCAACATCATAATCAAGTT380.10230729881808145No Hit
GTTATTAATAGGAATATGGATCAATTTCACAAAGAAGCCAATGAATCCCA380.10230729881808145No Hit
GGTATCAACGCAGAGTACATGGGGCATCTAGACCTAGCCGGGGCTGGCGT380.10230729881808145No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)