FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37143
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGCATCTAGACCTAGCCGGGGCTGGCGTAGTAGTAGGACATC1510.4065368979350079No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.38230622189914654No Hit
GTAGTAGGACATCTGTTTCTCGCCATCGTGATACAGAGAACACCCGAAGC1360.36615243787523893No Hit
GTCCAAGACCGGTTCATTGCAGCTCATTTGCAAGTCAGAACCAAATACAG1350.36346014053792103No Hit
GTACATGGGCAGCAAGCTCCAGTGCTACGTGTCCCTGGCATTTTAGGTGT1280.34461405917669546No Hit
GTATTTGGTTCTGACTTGCAAATGAGCTGCAATGAACCGGTCTTGGACCG1270.34192176183937756No Hit
CCCTCACACTTGTTCACCAATCTCTAAGATGAAAAACTCTCTTCCAAAAT1120.3015373017796085No Hit
ATCTAGACCTAGCCGGGGCTGGCGTAGTAGTAGGACATCTGTTTCTCGCC1120.3015373017796085No Hit
GTATCAACGCAGAGTACATGGGCAGCAAGCTCCAGTGCTACGTGTCCCTG970.26115284171983955No Hit
TTTGTATTCTGTGAGTTTCTCACACCCTCACACTTGTTCACCAATCTCTA940.2530759497078857No Hit
ACCAAATACAGACCAACTTGATTATGATGTTGGAGAAGAGCATCAGTCTC930.2503836523705678No Hit
TCCTATTGCTGTTGCCATGGCAATCTTCTGGAATTCTTTTCTATCAGGTT840.22615297633470638No Hit
GAGTACATGGGGCATCTAGACCTAGCCGGGGCTGGCGTAGTAGTAGGACA830.22346067899738845No Hit
TCTTTGAATGGTATCTCCTCTTCACTGATTAACATCTCTTCTTCCTCCTC820.2207683816600705No Hit
GTATCAACGCAGAGTACATGGGGCATCTAGACCTAGCCGGGGCTGGCGTA820.2207683816600705No Hit
GTCATGGATCAGGTAATGCAGTTTGTTGAGCCAAGTCGGCAGTTTGTAAA780.20999919231079878No Hit
CCTCTTCACTGATTAACATCTCTTCTTCCTCCTCCTCTTCCTCTTCACTA780.20999919231079878No Hit
GTACATGGGGAAGCTCCCAGAGACTGGTTTTCTTGGGATGCAGGCAGAAG770.20730689497348084No Hit
GATTTTCTCCGTGGCTTTGGGGTTTCCCTTTCTAAGTGGGGTTTGTAGGT760.2046145976361629No Hit
GTGATACAGAGAACACCCGAAGCTCTCGGTCCAAGACCGGTTCATTGCAG730.1965377056242091No Hit
GGTTAAAAGATGCACTAAACCTGATAGAAAAGAATTCCAGAAGATTGCCA720.1938454082868912No Hit
GCTTTGGGGTTTCCCTTTCTAAGTGGGGTTTGTAGGTCTCATCTCTTGGA710.19115311094957327No Hit
GTACATGGGCACATGGGACAGATGTAAAAGGGTCCAAGTTGCCACATTGC710.19115311094957327No Hit
CTACTAATGCCACCTGGAGACTGATGCTCTTCTCCAACATCATAATCAAG700.18846081361225533No Hit
ACGCAGAGTACATGGGCAGCAAGCTCCAGTGCTACGTGTCCCTGGCATTT690.1857685162749374No Hit
TTGTAAAGGACTCCATTCGGCTGGTTAAAAGATGCACTAAACCTGATAGA690.1857685162749374No Hit
GAATATGGATCAATTTCACAAAGAAGCCAATGAATCCCATTATAGCAAAT680.18307621893761947No Hit
TCGTGATACAGAGAACACCCGAAGCTCTCGGTCCAAGACCGGTTCATTGC670.18038392160030153No Hit
GAGTACATGGGCAGCAAGCTCCAGTGCTACGTGTCCCTGGCATTTTAGGT660.1776916242629836No Hit
CTCTTGGATCATCTTTGAATGGTATCTCCTCTTCACTGATTAACATCTCT660.1776916242629836No Hit
ATTAATAGGAATATGGATCAATTTCACAAAGAAGCCAATGAATCCCATTA600.161537840239076No Hit
CTCCAGTGCTACGTGTCCCTGGCATTTTAGGTGTCGGTTGGGTAGGCAGT590.15884554290175806No Hit
GGTTTAGTGCATCTTTTAACCAGCCGAATGGAGTCCTTTACAAACTGCCG580.15615324556444013No Hit
CCTGGAGACTGATGCTCTTCTCCAACATCATAATCAAGTTGGTCTGTATT580.15615324556444013No Hit
TCTCACACCCTCACACTTGTTCACCAATCTCTAAGATGAAAAACTCTCTT550.14807635355248633No Hit
TAGGAATATGGATCAATTTCACAAAGAAGCCAATGAATCCCATTATAGCA540.1453840562151684No Hit
CCATTCGGCTGGTTAAAAGATGCACTAAACCTGATAGAAAAGAATTCCAG530.14269175887785046No Hit
GGTATCAACGCAGAGTACATGGGGCATCTAGACCTAGCCGGGGCTGGCGT530.14269175887785046No Hit
GCAATAGGATTTGCTATAATGGGATTCATTGGCTTCTTTGTGAAATTGAT520.13999946154053253No Hit
GGTATCAACGCAGAGTACATGGGCAGCAAGCTCCAGTGCTACGTGTCCCT510.1373071642032146No Hit
GTATAATGTCCCTCAAGTTTATGGCAGTGTACCTTGTGCCACTGAATTTC510.1373071642032146No Hit
CACTAAACCTGATAGAAAAGAATTCCAGAAGATTGCCATGGCAACAGCAA510.1373071642032146No Hit
GTTATTAATAGGAATATGGATCAATTTCACAAAGAAGCCAATGAATCCCA510.1373071642032146No Hit
GTTCATTGCAGCTCATTTGCAAGTCAGAACCAAATACAGACCAACTTGAT500.13461486686589666No Hit
GGTAATGCAGTTTGTTGAGCCAAGTCGGCAGTTTGTAAAGGACTCCATTC500.13461486686589666No Hit
ACAATGATGTTATTAATAGGAATATGGATCAATTTCACAAAGAAGCCAAT480.1292302721912608No Hit
CCCCAGGACAGGGGAGAGGGATCGTCCTCATTCAGACTCTAGCTGGGGCC480.1292302721912608No Hit
GATTAACATCTCTTCTTCCTCCTCCTCTTCCTCTTCACTACTAATGCCAC480.1292302721912608No Hit
GTGCTACGTGTCCCTGGCATTTTAGGTGTCGGTTGGGTAGGCAGTCATGG480.1292302721912608No Hit
GTACTGGCTTCTCCCTGGGTGGGGTTGCCTGTTACATAGCTGTGCCTCAG470.12653797485394286No Hit
GCCACCAACAATGATGTTATTAATAGGAATATGGATCAATTTCACAAAGA450.12115338017930699No Hit
TTACATAGCTGTGCCTCAGAGAAAGGGTCCTGCATTTTCTGGAATGTTCT440.11846108284198906No Hit
GTCCTGGGGAGCCTGGGGTCACCTTGCAGAACAAGATGGTCCCAGGACTC440.11846108284198906No Hit
GTCTTGGACCGAGAGCTTCGGGTGTTCTCTGTATCACGATGGCGAGAAAC430.11576878550467112No Hit
TATCAACGCAGAGTACATGGGGCATCTAGACCTAGCCGGGGCTGGCGTAG430.11576878550467112No Hit
ACGCAGAGTACATGGGGCATCTAGACCTAGCCGGGGCTGGCGTAGTAGTA430.11576878550467112No Hit
GTCTCGTTCTGTTGCCCAGGCTGGAGTGCAGTGGCACAATCTCAGCTCAC420.11307648816735319No Hit
GTTAAAAGATGCACTAAACCTGATAGAAAAGAATTCCAGAAGATTGCCAT420.11307648816735319No Hit
CTCTTCTTCCTCCTCCTCTTCCTCTTCACTACTAATGCCACCTGGAGACT410.11038419083003526No Hit
TATCAACGCAGAGTACATGGGCAGCAAGCTCCAGTGCTACGTGTCCCTGG410.11038419083003526No Hit
GATGTAAAAGGGTCCAAGTTGCCACATTGCTTCATTAAAACAAGAGACCA400.10769189349271732No Hit
GCTATGTAACAGGCAACCCCACCCAGGGAGAAGCCAGTACAGAGGCCCCA400.10769189349271732No Hit
CTTCACTACTAATGCCACCTGGAGACTGATGCTCTTCTCCAACATCATAA400.10769189349271732No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)