FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29501
Sequences flagged as poor quality0
Sequence length100
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT332011.253855801498254No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT9983.382936171655198RNA PCR Primer, Index 31 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8472.871089115623199No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT7312.4778821056913323RNA PCR Primer, Index 31 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7302.4744923900884714No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5711.9355276092335851No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG4671.5829971865360497RNA PCR Primer, Index 31 (96% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4161.4101216907901428No Hit
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT3021.0236941120639977RNA PCR Primer, Index 31 (96% over 27bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.9796278092268058No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGT2840.9626792312125012No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT2260.766075726246568No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2010.6813328361750449No Hit
CTAATATACTATGAGATGAAAAGTACTTGTCAGGGATTTTCAAGCTTTTG1930.6542151113521575No Hit
GGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTT1860.6304871021321311No Hit
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG1690.5728619368834955RNA PCR Primer, Index 38 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.5694722212806346No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG1570.5321853496491644No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA1290.4372733127690587No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.39320700993186675No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTAGCTCGTATGCCGT1130.383037863123284No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1070.3626995695061184No Hit
GTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAAC1040.3525304226975357No Hit
GTATCAACGCAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTT1030.34914070709467476No Hit
GTATCAACGCAGAGTACTTCTGTTTGGCTTTTTTTCCCCACACCAATGGT1020.3457509914918138No Hit
ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC1010.34236127588895293RNA PCR Primer, Index 38 (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC990.33558184468323105No Hit
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA990.33558184468323105RNA PCR Primer, Index 31 (96% over 29bp)
CCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA980.33219212908037016No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.31863326666892644Clontech SMART CDS Primer II A (100% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCC900.3050744042574828No Hit
ACGCAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGT880.29829497305176095No Hit
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC870.29490525744890006No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG840.2847361106403173No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC830.2813463950374564No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT820.27795667943459546No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTACCTCGTATGCCGT810.2745669638317345No Hit
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT800.2711772482288736RNA PCR Primer, Index 31 (95% over 21bp)
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT800.2711772482288736No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC800.2711772482288736No Hit
TTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTC770.26100810142029085No Hit
CACCAATGGTAATTTATCTTCACAGATTGTTCTTCATTTCTAGAAATTGT770.26100810142029085No Hit
TTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGC730.24744923900884716No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT710.2406698078031253No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.2372800922002644No Hit
GAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGG700.2372800922002644No Hit
GAGTACTTCTGTTTGGCTTTTTTTCCCCACACCAATGGTAATTTATCTTC690.2338903765974035No Hit
GAGTACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGG690.2338903765974035No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG680.23050066099454253No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGTCTT680.23050066099454253No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTT630.21355208298023798No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC630.21355208298023798No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGTCTTCT600.2033829361716552RNA PCR Primer, Index 38 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC590.19999322056879426No Hit
CCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTT580.19660350496593337No Hit
CTATATTATTCATAAAATGACTGAGTGAAAACTAATATACTATGAGATGA580.19660350496593337No Hit
TATCAACGCAGAGTACTTCTGTTTGGCTTTTTTTCCCCACACCAATGGTA570.19321378936307243No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCCATCTCGTATGCCGT560.18982407376021151No Hit
TATCAACGCAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTA560.18982407376021151No Hit
TCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGG560.18982407376021151No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT560.18982407376021151No Hit
GGTATCAACGCAGAGTACTTCTGTTTGGCTTTTTTTCCCCACACCAATGG560.18982407376021151No Hit
CTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACT550.1864343581573506No Hit
GTACTTCTGTTTGGCTTTTTTTCCCCACACCAATGGTAATTTATCTTCAC550.1864343581573506No Hit
ACGCAGAGTACTTCTGTTTGGCTTTTTTTCCCCACACCAATGGTAATTTA540.18304464255448968No Hit
CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC530.17965492695162874No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG530.17965492695162874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC530.17965492695162874No Hit
CTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC510.1728754957459069No Hit
GTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAA510.1728754957459069No Hit
GGTATCAACGCAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATT510.1728754957459069No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTA500.169485780143046No Hit
AGTAATTACCATGAAGTAACAATTTCTAGAAATGAAGAACAATCTGTGAA490.16609606454018508No Hit
GCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTT490.16609606454018508No Hit
GCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCT480.16270634893732416No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC480.16270634893732416No Hit
ATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT470.15931663333446322No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC470.15931663333446322No Hit
AACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC420.14236805532015864No Hit
CAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCT410.13897833971729773No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.13897833971729773No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC410.13897833971729773No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC400.1355886241144368No Hit
GAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGG390.13219890851157587No Hit
GGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTAT380.12880919290871495No Hit
GTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGAT380.12880919290871495No Hit
ATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAA380.12880919290871495No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA360.12202976170299312No Hit
GGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAG360.12202976170299312No Hit
CAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGT350.1186400461001322No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCTACG350.1186400461001322No Hit
GCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGT350.1186400461001322No Hit
ACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA350.1186400461001322No Hit
GGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCT350.1186400461001322No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCTACGC340.11525033049727126No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACGCTACGC340.11525033049727126No Hit
GATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCC340.11525033049727126No Hit
ATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGA340.11525033049727126No Hit
TAAAATGACTGAGTGAAAACTAATATACTATGAGATGAAAAGTACTTGTC330.11186061489441036No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTAGCTCGTATGCCGTCTT330.11186061489441036No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC330.11186061489441036No Hit
CTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTT320.10847089929154943No Hit
GAAAAGTACTTGTCAGGGATTTTCAAGCTTTTGCTCAAGTAATTACCATG320.10847089929154943No Hit
ACTATATTATTCATAAAATGACTGAGTGAAAACTAATATACTATGAGATG310.10508118368868852No Hit
GTAATTACTTGAGCAAAAGCTTGAAAATCCCTGACAAGTACTTTTCATCT300.1016914680858276No Hit
TAACTATATTATTCATAAAATGACTGAGTGAAAACTAATATACTATGAGA300.1016914680858276No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)