FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29501
Sequences flagged as poor quality0
Sequence length100
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12914.376122843293448No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2510.8508186163180909No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT2400.8135317446866208No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.7355682858208196No Hit
GGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTT2050.6948916985864886No Hit
CTAATATACTATGAGATGAAAAGTACTTGTCAGGGATTTTCAAGCTTTTG2030.6881122673807668No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG1630.55252364326633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.508457340429138No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA1340.4542218907833633No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.41354530354903224No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1200.4067658723433104No Hit
GTATCAACGCAGAGTACTTCTGTTTGGCTTTTTTTCCCCACACCAATGGT1150.3898172943290058No Hit
GTATCAACGCAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTT1140.38642757872614486No Hit
GTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAAC1110.3762584319175621No Hit
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC1080.36608928510897937No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG1070.3626995695061184No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT1050.3559201383003966No Hit
TTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGC1020.3457509914918138No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.34236127588895293No Hit
CCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA920.31185383546320466No Hit
ACGCAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGT870.29490525744890006No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT850.2881258262431782No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC830.2813463950374564No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC820.27795667943459546No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG820.27795667943459546No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT800.2711772482288736No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG780.26439781702315174No Hit
TTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTC770.26100810142029085No Hit
TATCAACGCAGAGTACTTCTGTTTGGCTTTTTTTCCCCACACCAATGGTA760.2576183858174299No Hit
GTACTTCTGTTTGGCTTTTTTTCCCCACACCAATGGTAATTTATCTTCAC760.2576183858174299No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTA730.24744923900884716No Hit
GGTATCAACGCAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATT720.24405952340598625No Hit
GAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGG720.24405952340598625No Hit
GGTATCAACGCAGAGTACTTCTGTTTGGCTTTTTTTCCCCACACCAATGG700.2372800922002644No Hit
GAGTACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGG680.23050066099454253No Hit
GTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAA670.22711094539168164No Hit
TCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGG670.22711094539168164No Hit
GAGTACTTCTGTTTGGCTTTTTTTCCCCACACCAATGGTAATTTATCTTC660.22372122978882072No Hit
GTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGAT660.22372122978882072No Hit
CACCAATGGTAATTTATCTTCACAGATTGTTCTTCATTTCTAGAAATTGT640.21694179858309887No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT630.21355208298023798No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA620.21016236737737704No Hit
CCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTT610.20677265177451612No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTT580.19660350496593337No Hit
CTATATTATTCATAAAATGACTGAGTGAAAACTAATATACTATGAGATGA550.1864343581573506No Hit
TATCAACGCAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTA550.1864343581573506No Hit
GCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTT500.169485780143046No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.169485780143046No Hit
TAAAATGACTGAGTGAAAACTAATATACTATGAGATGAAAAGTACTTGTC490.16609606454018508No Hit
ATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT480.16270634893732416No Hit
AACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC460.15592691773160233No Hit
GGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAG460.15592691773160233No Hit
GGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCT460.15592691773160233No Hit
GCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCT450.1525372021287414No Hit
GAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGA450.1525372021287414No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC450.1525372021287414No Hit
TTCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTA450.1525372021287414No Hit
AGTAATTACCATGAAGTAACAATTTCTAGAAATGAAGAACAATCTGTGAA440.14914748652588047No Hit
ACGCAGAGTACTTCTGTTTGGCTTTTTTTCCCCACACCAATGGTAATTTA440.14914748652588047No Hit
ATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAA440.14914748652588047No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.14914748652588047No Hit
CTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACT430.14575777092301956No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.13897833971729773No Hit
ATATTATTCATAAAATGACTGAGTGAAAACTAATATACTATGAGATGAAA400.1355886241144368No Hit
GGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTAT390.13219890851157587No Hit
CAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGT380.12880919290871495No Hit
ATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACC380.12880919290871495No Hit
TCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTA370.12541947730585404No Hit
ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.12541947730585404No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.12202976170299312No Hit
GAAAAGTACTTGTCAGGGATTTTCAAGCTTTTGCTCAAGTAATTACCATG360.12202976170299312No Hit
GCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGT350.1186400461001322No Hit
ATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCG350.1186400461001322No Hit
GTATCAACGCAGAGTACACCCGTCTATGTAGCAAAATAGTGGGAAGATTT340.11525033049727126No Hit
ACTATATTATTCATAAAATGACTGAGTGAAAACTAATATACTATGAGATG340.11525033049727126No Hit
GTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGA330.11186061489441036No Hit
GTAATTACTTGAGCAAAAGCTTGAAAATCCCTGACAAGTACTTTTCATCT330.11186061489441036No Hit
ACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA330.11186061489441036No Hit
TAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGC330.11186061489441036No Hit
CTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC330.11186061489441036No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC330.11186061489441036No Hit
GATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCC320.10847089929154943No Hit
CAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCT320.10847089929154943No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.10508118368868852No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.10508118368868852No Hit
CTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTT310.10508118368868852No Hit
CCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAG300.1016914680858276No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.1016914680858276No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)