FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28138
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT20727.363707441893524No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT7392.6263416021039165TruSeq Adapter, Index 10 (95% over 21bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG6302.238965100575734TruSeq Adapter, Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT5361.9048972919184022RNA PCR Primer, Index 10 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.9915416873978251No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGT2420.8600469116497262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250.7996303930627622No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT2130.7569834387660814TruSeq Adapter, Index 10 (96% over 26bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA2130.7569834387660814RNA PCR Primer, Index 10 (96% over 28bp)
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG2040.724998223043571No Hit
GTATCAACGCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATC1710.6077190987276991No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.49399388726988414No Hit
GTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACAT1320.46911649726348714No Hit
ACGCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC1310.4655625844054304No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA1230.43713128154097664No Hit
TATCAACGCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCT1230.43713128154097664No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG1210.4300234558248631TruSeq Adapter, Index 10 (95% over 24bp)
GGTATCAACGCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT1110.39448432724429594No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT1080.3838225886701258No Hit
GTACAAGCACCCGCTCCCTGGGGTACAGACCAGTGGGGGACATGAGAAAG1070.3802686758120691No Hit
GTACATGGGGTCACCAGGCTGGAGTGCAGTGGCGCGATCTCGGCACACCT1050.3731608500959556No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG1030.3660530243798422No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC1000.35539128580567203No Hit
TCCCTGTACTCCCCAAGGACAAGGCTGCAGGAAGAGGGATCCCCAGGAAG1000.35539128580567203No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA970.34472954723150184No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGT900.31985215722510485No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTT860.30563650579287793No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC800.2843130286445376No Hit
GAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAAC780.27720520292842415No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC780.27720520292842415Illumina Paired End PCR Primer 2 (95% over 24bp)
GCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTC710.25232781292202716No Hit
GTACATGGGGGACTTCAGGTTGATGCTGGAGACGTGCTACCGCCTGCACG690.2452199872059137No Hit
ATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGT660.23455824863174357No Hit
TACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGC650.23100433577368681No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT650.23100433577368681No Hit
AGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCC600.21323477148340325No Hit
GAGTGGACCACTGGATCTCCAAACAGGGCCAGAAGCTGGAGATGATGCTG600.21323477148340325No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.2096808586253465No Hit
GATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATC580.2061269457672898No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC560.19901912005117636No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGACCTCGTATGCCGT550.1954652071931196No Hit
TCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTC530.1883573814770062No Hit
GCCCAGCCCCATCCGCAAGCCAGGCTGGGCCTGGGCATAACTTCGCTGGG520.18480346861894947No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCT520.18480346861894947Illumina PCR Primer Index 5 (95% over 21bp)
GAGTACATGGGGGACTTCAGGTTGATGCTGGAGACGTGCTACCGCCTGCA510.18124955576089274No Hit
ATATTAGTTGGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATG510.18124955576089274No Hit
CTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCAC500.17769564290283602No Hit
GTATCAACGCAGAGTACATGGGGGACTTCAGGTTGATGCTGGAGACGTGC480.17058781718672258No Hit
GATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGGAAAGT470.16703390432866586No Hit
TCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCTGCTTG470.16703390432866586Illumina PCR Primer Index 5 (96% over 26bp)
CCATTATAGATTCAAAAACTCCACTTGTCAAGCCTGCCAAACTTCGCGGT460.16347999147060915No Hit
TTTCTTTCCTGCTCCCGAAGCCTTTTTTCTTCTTTTGCTCTCAGCTGTTC460.16347999147060915No Hit
GGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTG440.1563721657544957No Hit
GGGTTTCACCATGTTGGCCAGGATGGTCTCGATCTCCTGACCTCATGATC430.15281825289643897No Hit
GTATCAACGCAGAGTACATGGGGTCACCAGGCTGGAGTGCAGTGGCGCGA430.15281825289643897No Hit
ATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGG420.14926434003838226No Hit
GAGTACATGGGGTCACCAGGCTGGAGTGCAGTGGCGCGATCTCGGCACAC420.14926434003838226No Hit
CTCCCAAGACCATCAGCAGTGCATCCAGCCCCCTGGGGCCTGGGCTGCTG420.14926434003838226No Hit
GTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAA410.14571042718032554No Hit
AGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAG410.14571042718032554No Hit
GACTATAGGTGCATGCCACCATGCCCAGCTAATTTTTGTATTTTTAGTAG410.14571042718032554No Hit
GGGCTGGGCTGGTCAGTGAGGATCCCCACAGTACGGGCTCTGGAATCATC410.14571042718032554No Hit
TCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCC400.1421565143222688No Hit
AACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGT400.1421565143222688No Hit
CCCCAAGGACAAGGCTGCAGGAAGAGGGATCCCCAGGAAGGAGTGTAATT400.1421565143222688No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.1421565143222688No Hit
AGCTTCTTCTGCTTCTTTTCTTTCCTGCTCCCGAAGCCTTTTTTCTTCTT390.13860260146421208No Hit
GTACAGGGAAGTACCTGCAGTGACCCTGCCCCCATGACCTTGGCCAGGCA390.13860260146421208No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGT390.13860260146421208No Hit
GGTATCAACGCAGAGTACATGGGGGACTTCAGGTTGATGCTGGAGACGTG380.13504868860615538No Hit
ACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTC370.13149477574809865No Hit
CAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTT370.13149477574809865No Hit
CTTGATACACAAAGGAGGGCGGAGAGCAGAGCAGCCCTGGCCGAGGCTAG370.13149477574809865No Hit
GTGATGTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAG370.13149477574809865No Hit
GAATGGGGCTGTGAGCGACACTTGATACACAAAGGAGGGCGGAGAGCAGA370.13149477574809865No Hit
CCATAATAGCCTCTAGATGTAACTGCTATCGTTGTCTTTTCTCTCAAGTG370.13149477574809865No Hit
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT360.12794086289004192No Hit
GCCCTGGCCGAGGCTAGAGACCAAGGGACTGATGAAGAACCAGCAGCAGC360.12794086289004192No Hit
ATACATGATTGGTGCCAAGGAAGTCATTAAGTGTGAATGTGAACACTGGA360.12794086289004192No Hit
CCTTCCTGGGGATCCCTCTTCCTGCAGCCTTGTCCTTGGGGAGTACAGGG350.1243869500319852No Hit
GAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGC350.1243869500319852No Hit
GCCCTGTTGTCTTTCAAAGAACCTCGGCCCCAGTGATGGCTGATCTCACT350.1243869500319852No Hit
CCTTTGTGTATCAAGTGTCGCTCACAGCCCCATTCACTGGGGAGCCTTTC340.1208330371739285No Hit
TCTCCAAACAGGGCCAGAAGCTGGAGATGATGCTGGAGCAGAAGTTAGCG340.1208330371739285No Hit
ACACAAAGGAGGGCGGAGAGCAGAGCAGCCCTGGCCGAGGCTAGAGACCA340.1208330371739285No Hit
GTGAAATACATGATTGGTGCCAAGGAAGTCATTAAGTGTGAATGTGAACA330.11727912431587179No Hit
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC330.11727912431587179No Hit
GTACATGGGGCCTCACACCACATACAAAAAATCAACTCACGATGAATCAA330.11727912431587179No Hit
CAGTACAAGCACCCGCTCCCTGGGGTACAGACCAGTGGGGGACATGAGAA330.11727912431587179No Hit
CTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC330.11727912431587179No Hit
TGTCAAGCCTGCCAAACTTCGCGGTGTTGACCGACGCCTTGTTGAGGTAC330.11727912431587179No Hit
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGG320.11372521145781506No Hit
CTACGAGACAGTGGGAGTGAGATCAGCCATCACTGGGGCCGAGGTTCTTT320.11372521145781506No Hit
GGTATCAACGCAGAGTACATGGGGTCACCAGGCTGGAGTGCAGTGGCGCG320.11372521145781506No Hit
GTCTTGGGAGAGCCCAGCGAAGTTATGCCCAGGCCCAGCCTGGCTTGCGG320.11372521145781506No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.11017129859975834No Hit
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAA310.11017129859975834TruSeq Adapter, Index 10 (96% over 28bp)
CTATAGGTGCATGCCACCATGCCCAGCTAATTTTTGTATTTTTAGTAGAG310.11017129859975834No Hit
ACATGGGGGACTTCAGGTTGATGCTGGAGACGTGCTACCGCCTGCACGGA310.11017129859975834No Hit
GTGGGGGACATGAGAAAGACCAAATAGATCCGAAAGGCTCCCCAGTGAAT300.10661738574170163No Hit
CCCCAGGAAGGAGTGTAATTCTACGAGACAGTGGGAGTGAGATCAGCCAT300.10661738574170163No Hit
GTGCCAAGGAAGTCATTAAGTGTGAATGTGAACACTGGATTCACACAGTA300.10661738574170163No Hit
GCTGATGGTCTTGGGAGAGCCCAGCGAAGTTATGCCCAGGCCCAGCCTGG300.10661738574170163No Hit
GACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGG300.10661738574170163No Hit
CCCCAGTGAATGGGGCTGTGAGCGACACTTGATACACAAAGGAGGGCGGA300.10661738574170163No Hit
TTCCTGGGGATCCCTCTTCCTGCAGCCTTGTCCTTGGGGAGTACAGGGAA300.10661738574170163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG300.10661738574170163No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTT300.10661738574170163No Hit
CCTCCGCCTTCTGGGTTCAAGCAATTCTCCTGCCTCAGCCTCCTGAGTAG290.1030634728836449No Hit
GTGTGAATGTGAACACTGGATTCACACAGTACAAGCACCCGCTCCCTGGG290.1030634728836449No Hit
CACTCCCACTGTCTCGTAGAATTACACTCCTTCCTGGGGATCCCTCTTCC290.1030634728836449No Hit
CTCTTTATCCTACAAGCTGTTTCAGTAGGTTTTGTAGTTTTAGCTCTTAC290.1030634728836449No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.1030634728836449Clontech SMART CDS Primer II A (100% over 21bp)
CTAATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGC290.1030634728836449No Hit
ACGCAGAGTACATGGGGGACTTCAGGTTGATGCTGGAGACGTGCTACCGC290.1030634728836449No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)