FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28138
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG2110.749875613049968No Hit
GTATCAACGCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATC2100.7463217001919112No Hit
ACGCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC1480.5259791029923946No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA1390.49399388726988414No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1330.4726704101215438No Hit
GGTATCAACGCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT1260.44779302011514677No Hit
TATCAACGCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCT1260.44779302011514677No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA1210.4300234558248631No Hit
GTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACAT1150.40869997867652286No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG1140.40514606581846613No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC1120.3980382401023527No Hit
GAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAAC1120.3980382401023527No Hit
GTACAAGCACCCGCTCCCTGGGGTACAGACCAGTGGGGGACATGAGAAAG1070.3802686758120691No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT990.3518373729476153No Hit
GCCCAGCCCCATCCGCAAGCCAGGCTGGGCCTGGGCATAACTTCGCTGGG970.34472954723150184No Hit
TCCCTGTACTCCCCAAGGACAAGGCTGCAGGAAGAGGGATCCCCAGGAAG930.33051389579927504No Hit
GTACATGGGGTCACCAGGCTGGAGTGCAGTGGCGCGATCTCGGCACACCT840.29852868007676453No Hit
ATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGT800.2843130286445376No Hit
GTACATGGGGGACTTCAGGTTGATGCTGGAGACGTGCTACCGCCTGCACG790.28075911578648094No Hit
GTACAGGGAAGTACCTGCAGTGACCCTGCCCCCATGACCTTGGCCAGGCA740.2629895514961973No Hit
TACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGC690.2452199872059137No Hit
GCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTC660.23455824863174357No Hit
GTGGGAGTGAGATCAGCCATCACTGGGGCCGAGGTTCTTTGAAAGACAAC590.2096808586253465No Hit
CTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCAC570.20257303290923306No Hit
GTACATGGGGCCTCACACCACATACAAAAAATCAACTCACGATGAATCAA560.19901912005117636No Hit
GAGTGGACCACTGGATCTCCAAACAGGGCCAGAAGCTGGAGATGATGCTG560.19901912005117636No Hit
CCCCAAGGACAAGGCTGCAGGAAGAGGGATCCCCAGGAAGGAGTGTAATT550.1954652071931196No Hit
ATATTAGTTGGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATG550.1954652071931196No Hit
GGGCTGGGCTGGTCAGTGAGGATCCCCACAGTACGGGCTCTGGAATCATC550.1954652071931196No Hit
GATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATC540.1919112943350629No Hit
AGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCC530.1883573814770062No Hit
CTCCCAAGACCATCAGCAGTGCATCCAGCCCCCTGGGGCCTGGGCTGCTG530.1883573814770062No Hit
AACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGT520.18480346861894947No Hit
TCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTC500.17769564290283602No Hit
CCATTATAGATTCAAAAACTCCACTTGTCAAGCCTGCCAAACTTCGCGGT500.17769564290283602No Hit
GATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGGAAAGT500.17769564290283602No Hit
CTATAGGTGCATGCCACCATGCCCAGCTAATTTTTGTATTTTTAGTAGAG490.1741417300447793No Hit
GGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTG480.17058781718672258No Hit
GTATCAACGCAGAGTACATGGGGGACTTCAGGTTGATGCTGGAGACGTGC470.16703390432866586No Hit
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT460.16347999147060915No Hit
GAGTACATGGGGTCACCAGGCTGGAGTGCAGTGGCGCGATCTCGGCACAC460.16347999147060915No Hit
GTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAA440.1563721657544957No Hit
AGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAG440.1563721657544957No Hit
GACTATAGGTGCATGCCACCATGCCCAGCTAATTTTTGTATTTTTAGTAG440.1563721657544957No Hit
GTATCAACGCAGAGTACATGGGGTCACCAGGCTGGAGTGCAGTGGCGCGA420.14926434003838226No Hit
GAGTACATGGGGGACTTCAGGTTGATGCTGGAGACGTGCTACCGCCTGCA420.14926434003838226No Hit
GAGAAGAACAGAGTGTTCGATTCTGCCCTATTTATGTTTCTACTCGGGAA420.14926434003838226No Hit
AGCTTCTTCTGCTTCTTTTCTTTCCTGCTCCCGAAGCCTTTTTTCTTCTT400.1421565143222688No Hit
GTGAAATACATGATTGGTGCCAAGGAAGTCATTAAGTGTGAATGTGAACA390.13860260146421208No Hit
GTGTGAATGTGAACACTGGATTCACACAGTACAAGCACCCGCTCCCTGGG390.13860260146421208No Hit
ATTATGGATTGGAGGATGAGAAGGGAACTGCATGTACCTCAACAAGGCGT390.13860260146421208No Hit
ATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGG380.13504868860615538No Hit
GAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGC380.13504868860615538No Hit
CTAATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGC370.13149477574809865No Hit
TTTCTTTCCTGCTCCCGAAGCCTTTTTTCTTCTTTTGCTCTCAGCTGTTC370.13149477574809865No Hit
GACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGC360.12794086289004192No Hit
ACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTC360.12794086289004192No Hit
GGGTTTCACCATGTTGGCCAGGATGGTCTCGATCTCCTGACCTCATGATC360.12794086289004192No Hit
GGTCAGTGAGGATCCCCACAGTACGGGCTCTGGAATCATCCTGCCTGGCC360.12794086289004192No Hit
AAATATTAGTTGGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAA360.12794086289004192No Hit
CAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTT350.1243869500319852No Hit
GACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGG350.1243869500319852No Hit
TTCCTGGGGATCCCTCTTCCTGCAGCCTTGTCCTTGGGGAGTACAGGGAA350.1243869500319852No Hit
AACTAATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCC350.1243869500319852No Hit
CTCCTGACCTCATGATCCACCCACCTCGGCCTCCCAAAGTGCTGGGATTC340.1208330371739285No Hit
TATCAACGCAGAGTACATGGGGTCACCAGGCTGGAGTGCAGTGGCGCGAT340.1208330371739285No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG340.1208330371739285No Hit
GGATTCACACAGTACAAGCACCCGCTCCCTGGGGTACAGACCAGTGGGGG340.1208330371739285No Hit
TCCTTGGGGAGTACAGGGAAGTACCTGCAGTGACCCTGCCCCCATGACCT340.1208330371739285No Hit
GTACGGGCTCTGGAATCATCCTGCCTGGCCAAGGTCATGGGGGCAGGGTC340.1208330371739285No Hit
TCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATA340.1208330371739285No Hit
GGTATCAACGCAGAGTACATGGGGTCACCAGGCTGGAGTGCAGTGGCGCG340.1208330371739285No Hit
GAGTACATGGGGCCTCACACCACATACAAAAAATCAACTCACGATGAATC330.11727912431587179No Hit
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGG330.11727912431587179No Hit
ACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATC330.11727912431587179No Hit
GAGCAGCCCTGGCCGAGGCTAGAGACCAAGGGACTGATGAAGAACCAGCA330.11727912431587179No Hit
ATCCATAATAGCCTCTAGATGTAACTGCTATCGTTGTCTTTTCTCTCAAG330.11727912431587179No Hit
CCTTTGTGTATCAAGTGTCGCTCACAGCCCCATTCACTGGGGAGCCTTTC330.11727912431587179No Hit
GTGTTGACCGACGCCTTGTTGAGGTACATGCAGTTCCCTTCTCATCCTCC320.11372521145781506No Hit
GCCCTGTTGTCTTTCAAAGAACCTCGGCCCCAGTGATGGCTGATCTCACT320.11372521145781506No Hit
CCACCATGCCCAGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACC320.11372521145781506No Hit
GTGGGGGACATGAGAAAGACCAAATAGATCCGAAAGGCTCCCCAGTGAAT320.11372521145781506No Hit
CCATAATAGCCTCTAGATGTAACTGCTATCGTTGTCTTTTCTCTCAAGTG320.11372521145781506No Hit
CCCCAGGAAGGAGTGTAATTCTACGAGACAGTGGGAGTGAGATCAGCCAT310.11017129859975834No Hit
CTACGAGACAGTGGGAGTGAGATCAGCCATCACTGGGGCCGAGGTTCTTT310.11017129859975834No Hit
ACCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACA310.11017129859975834No Hit
CTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTGATACTGGC310.11017129859975834No Hit
CCTATAGTCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATTGCTTGAACC310.11017129859975834No Hit
GGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCA310.11017129859975834No Hit
ATACATGATTGGTGCCAAGGAAGTCATTAAGTGTGAATGTGAACACTGGA300.10661738574170163No Hit
TGTCAAGCCTGCCAAACTTCGCGGTGTTGACCGACGCCTTGTTGAGGTAC300.10661738574170163No Hit
GTGTAATTCTACGAGACAGTGGGAGTGAGATCAGCCATCACTGGGGCCGA300.10661738574170163No Hit
CACTCCCACTGTCTCGTAGAATTACACTCCTTCCTGGGGATCCCTCTTCC300.10661738574170163No Hit
CAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCA290.1030634728836449No Hit
GAATGGGGCTGTGAGCGACACTTGATACACAAAGGAGGGCGGAGAGCAGA290.1030634728836449No Hit
CAGTACAAGCACCCGCTCCCTGGGGTACAGACCAGTGGGGGACATGAGAA290.1030634728836449No Hit
GTCTTGGGAGAGCCCAGCGAAGTTATGCCCAGGCCCAGCCTGGCTTGCGG290.1030634728836449No Hit
GTACAGACCAGTGGGGGACATGAGAAAGACCAAATAGATCCGAAAGGCTC290.1030634728836449No Hit
TCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCC290.1030634728836449No Hit
GTGATGTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAG290.1030634728836449No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)