FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41797
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT26576.356915568102973No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT8742.091059166925856RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT6421.535995406368878RNA PCR Primer, Index 33 (95% over 24bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG6361.5216403091130943RNA PCR Primer, Index 33 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGT3830.9163337081608727No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT2600.6220542144173027RNA PCR Primer, Index 33 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.4880733066966529No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA2030.4856807904873556RNA PCR Primer, Index 33 (96% over 29bp)
GTCTAGATCACATTCTCGATCCAGAGGAAGGCGATACTCTCGCTCACGCA1930.46175562839438233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.4043352393712467No Hit
CACATAAACTGCGGGCTGAGTGAGCCTACAGACAATATGAGAAACCAGCA1620.3875876259061655No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG1530.36605498002248965RNA PCR Primer, Index 15 (95% over 24bp)
GATAAAGACAGCCAGGTCCATGGCCGGGCGCAGTGGCTCATGCCTGTAAT1270.3038495585807594No Hit
GTACATGGGAGTGAGGGAAGAGGCCACCAGGTGGCAGCACAGCCACACCC1270.3038495585807594No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGT1230.2942794937435701No Hit
CACTTACCCTGTGTTTAAAAAGATCTTGTACCAAGCCAACGGCGTTCCTG1110.2655692992320023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.263176783022705No Hit
GTTCAGATTTGTTCATAAAAGATATGTTACAGGAACATTTTAGAAATCAA1080.25839175060411035No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTT990.23685910472043448No Hit
GTCTTTATCATCCCCACAAACATTTTGAAACTGGAATATTTGTCTTCAGA960.22968155609254254No Hit
TCATTATGTTGGCCAGGCTGGTCTCGAACTCCTTACCTCAGGTGATCCAC960.22968155609254254No Hit
GATCTAGACCGTGACCTATTTTTCAAGTTAGAGAAAAAACAAAATGTCAG940.2248965236739479No Hit
GTCCTGGGTGGACGGCAGGTGAACACAAGCGGCTGCCGCATGTAGCCACT940.2248965236739479No Hit
GGGTAAGTGTTGGTAGCACGAGGGGCCTCTATTCCACACACAGGAGAGGT930.22250400746465057No Hit
ATCCAGAGGAAGGCGATACTCTCGCTCACGCAGCAGGAGCAGGGGACGAA900.21532645883675863No Hit
CCTTATAATAATGTAAACAAAATAACTTTTCCCTAAAGGGTGAACTTGAG890.2129339426274613No Hit
TAGTAATCCAGATCCATTTTGATTAGATGGTTGAATTATCTTTCCTAGGT870.20814891020886667No Hit
GGACAGGGCTTATCATTTATAGTCTTGTTTCCATTTTCTGAAGACAAATA860.20575639399956933No Hit
CATAAACTGCGGGCTGAGTGAGCCTACAGACAATATGAGAAACCAGCACA860.20575639399956933No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCT810.19379381295308276Illumina PCR Primer Index 4 (95% over 21bp)
CCCAAGTACCTGGGACTACAGGCGTGAGCTACCATGCCCGGCTAATTTTT800.19140129674378542No Hit
CCCTAGCACCACCTCTCCTGTGTGTGGAATAGAGGCCCCTCGTGCTACCA790.1890087805344881No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT770.18422374811589348RNA PCR Primer, Index 33 (95% over 21bp)
CAATAAAACTTTGTACACAGGAAGTAGCAAAATACATCATTTTTCATAGA770.18422374811589348No Hit
ATTATAAGGGATTTGTGATGTCTGTAAAGTGTAACCTAGGAAAGATAATT760.18183123190659617No Hit
CTATAAAAGGATCGAGGTATTTCCAATCCCCGTCGAGGTCAAGATCAAGA750.17943871569729886No Hit
ATCTTACTGCTGTGAATTTACATAGTAATCCAGATCCATTTTGATTAGAT730.1746536832787042No Hit
ACCCAGGACAGTCCCCGGGCTGGCTCCAGGCTGCAGGGGGTCAGCAGAGG730.1746536832787042No Hit
GTTTTGAGATTTGATTTCAGCAGAGGCACACAAACTCTTAAAAACGAGTT720.17226116706940692No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC720.17226116706940692No Hit
GTCCTGGGTGGAGGGCAGGTGAACACAAGCTGCTGCCGGGGACTGTCCTG720.17226116706940692No Hit
ATCTTGTACCAAGCCAACGGCGTTCCTGGCTCTCCTGCCCACAGGATGAA710.16986865086010958No Hit
GCCCTGTCCCTAGCACCACCTCTCCTGTGTGTGGAATAGAGGCCCCTCGT700.16747613465081226No Hit
GCCATGGACCTGGCTGTCTTTATCATCCCCACAAACATTTTGAAACTGGA700.16747613465081226No Hit
GCATAAAATATTCGGGGGAACCAGCACTTAAAAAACAGACTTCATGAAAG700.16747613465081226No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAACCTCGTATGCCGT700.16747613465081226No Hit
GAATAGAGGCCCCTCGTGCTACCAACACTTACCCTGTGTTTAAAAAGATC700.16747613465081226No Hit
CAATATGAGAAACCAGCACACGCTTTGGAATACGGTAGGGCAAAACTCCT690.16508361844151492No Hit
ACTTTGTACACAGGAAGTAGCAAAATACATCATTTTTCATAGAAAAAAGC690.16508361844151492No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC690.16508361844151492RNA PCR Primer, Index 15 (96% over 25bp)
ATTTATTTCAGAGCAGTTACACCCAGCAGAGGGGCACAGGCTTAAACGCC680.1626911022322176No Hit
GACCTAGATCTTCTGAGTGAAGCTGATCTTGATCTACGAAGAGAGATAGA650.15551355360432567No Hit
CTCCTAAGGAAGCCGAAAATGTTCATCCTGTGGGCAGGAGAGCCAGGAAC650.15551355360432567No Hit
AGGTAGAGTGCAGTGGCGTGATCTCTGCTCATTGCAACTGCCGCCTCCCG640.15312103739502836No Hit
GTACAAGATCTTTTTAAACACAGGGTAAGTGTTGGTAGCACGAGGGGCCT640.15312103739502836No Hit
GTTACACCCAGCAGAGGGGCACAGGCTTAAACGCCGGCATATTAGTTTTC630.15072852118573102No Hit
GGAAGTAGCAAAATACATCATTTTTCATAGAAAAAAGCACACACATAAAC630.15072852118573102No Hit
GTAGAGTGCAGTGGCGTGATCTCTGCTCATTGCAACTGCCGCCTCCCGGG610.1459434887671364No Hit
CTTTGGAATACGGTAGGGCAAAACTCCTAAGGAAGCCGAAAATGTTCATC610.1459434887671364No Hit
CTACTAGGCCCCCTTACTCCTAATTAATTGCCTGCTCACCAGACTGTGAG600.14355097255783908No Hit
GTCCCTAGCACCACCTCTCCTGTGTGTGGAATAGAGGCCCCTCGTGCTAC600.14355097255783908No Hit
ACCGTATTCCAAAGCGTGTGCTGGTTTCTCATATTGTCTGTAGGCTCACT590.14115845634854177No Hit
GTTTATGTGTGTGCTTTTTTCTATGAAAAATGATGTATTTTGCTACTTCC580.13876594013924445No Hit
ATGTTGGCCAGGCTGGTCTCGAACTCCTTACCTCAGGTGATCCACCCACC580.13876594013924445No Hit
ACAACTAATAGGCCCAGAGTTTATTTCCAAATGAGACACTAAATTTAAAT570.1363734239299471No Hit
TCCTGATGGGGAACGACTGCTTCTTGGTCGTGAAATAGACCTGGATCTTG570.1363734239299471No Hit
GTGTTTAAAAAGATCTTGTACCAAGCCAACGGCGTTCCTGGCTCTCCTGC560.1339809077206498No Hit
CTAATAGGCCCAGAGTTTATTTCCAAATGAGACACTAAATTTAAATAGTT550.1315883915113525No Hit
CCTTAGGAGTTTTGCCCTACCGTATTCCAAAGCGTGTGCTGGTTTCTCAT550.1315883915113525No Hit
ATCTAGGTCTGGTTCTATAAAAGGATCGAGGTATTTCCAATCCCCGTCGA540.12919587530205517No Hit
TCCCTAAAGGGTGAACTTGAGAGCTTCAGTCCATTCTTTCAGGACTTGCA530.12680335909275786No Hit
ATAATAATGTAAACAAAATAACTTTTCCCTAAAGGGTGAACTTGAGAGCT530.12680335909275786No Hit
ATTTTATGCAGAGGAGGGAAAATTTATAGTGGCAATTATTTTCTCACAGT520.12441084288346052No Hit
GTACATGGGAGTAAAAAAAAAATACTCAACAACTAATAGGCCCAGAGTTT520.12441084288346052No Hit
GTTTCACTCTGTTGCCCAAGGTAGAGTGCAGTGGCGTGATCTCTGCTCAT520.12441084288346052No Hit
ATTCCAAAGCGTGTGCTGGTTTCTCATATTGTCTGTAGGCTCACTCAGCC520.12441084288346052No Hit
ATTTAAATGTGCCAAATAAAAACCCACATTTTCAGACCATATGATGTTAA520.12441084288346052No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGC510.12201832667416322No Hit
ACCCAGGACAGTCCCCGGCAGCAGCTTGTGTTCACCTGCCCTCCACCCAG510.12201832667416322No Hit
CCTCTGGATCGAGAATGTGATCTAGACCGTGACCTATTTTTCAAGTTAGA510.12201832667416322No Hit
GTTCATGCAAAGCACAAAAAACATTTACAATAAAACTTTGTACACAGGAA500.11962581046486591No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC500.11962581046486591No Hit
ACCATGCCCGGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCATTAT500.11962581046486591No Hit
CCATTATAGTTACTGCACATAAGACTATTACTACTAAAGGTCACTTCAGA490.11723329425556857No Hit
TCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCTGCTTG490.11723329425556857Illumina PCR Primer Index 4 (96% over 26bp)
ATTCCACACACAGGAGAGGTGGTGCTAGGGACAGGGCTTATCATTTATAG490.11723329425556857No Hit
GGCTTAAACGCCGGCATATTAGTTTTCCCGCACGCGAGCCCTGCATGGCG480.11484077804627127No Hit
ACATGGGAGTGAGGGAAGAGGCCACCAGGTGGCAGCACAGCCACACCCGT470.11244826183697396No Hit
AGGTTACACTTTACAGACATCACAAATCCCTTATAATAATGTAAACAAAA470.11244826183697396No Hit
GGTCTATTTCACGACCAAGAAGCAGTCGTTCCCCATCAGGAAGTCCTCGC470.11244826183697396No Hit
GTTCTACTGAAACAATTGCAACAACGTGGCCCCTGTTCATGCAAAGCACA470.11244826183697396No Hit
ACATAATGAAACCCCATCTCTACTAAAAATACAAAAATTAGCCGGGCATG460.11005574562767662No Hit
CTTAAAAACGAGTTATTGTCTGACATTTTGTTTTTTCTCTAACTTGAAAA460.11005574562767662No Hit
GAGTGAGCCTACAGACAATATGAGAAACCAGCACACGCTTTGGAATACGG450.10766322941837932No Hit
GTATCAACGCAGAGTACATGGGAGTGAGGGAAGAGGCCACCAGGTGGCAG450.10766322941837932No Hit
AAATAGACCTGGATCTTGATCTTGACCTCGACGGGGATTGGAAATACCTC450.10766322941837932No Hit
GTGCTAGGGACAGGGCTTATCATTTATAGTCTTGTTTCCATTTTCTGAAG450.10766322941837932No Hit
CCTTACCTCAGGTGATCCACCCACCTTGGCCTCCCGAAGTGCTGGGATTA450.10766322941837932No Hit
CGTCAGAGGAGGGCAAGGCTGGGTACTCAGCAGCCACTCTGAGCCGGGGC450.10766322941837932No Hit
TTTATAGTCTTGTTTCCATTTTCTGAAGACAAATATTCCAGTTTCAAAAT440.105270713209082No Hit
CAAAATAACTTTTCCCTAAAGGGTGAACTTGAGAGCTTCAGTCCATTCTT440.105270713209082No Hit
GAGATGGGGTTTCATTATGTTGGCCAGGCTGGTCTCGAACTCCTTACCTC440.105270713209082No Hit
GAATACGGTAGGGCAAAACTCCTAAGGAAGCCGAAAATGTTCATCCTGTG440.105270713209082No Hit
GGGTTTCATTATGTTGGCCAGGCTGGTCTCGAACTCCTTACCTCAGGTGA430.10287819699978466No Hit
GTTCATAAAAGATATGTTACAGGAACATTTTAGAAATCAAACCAGTTCTA430.10287819699978466No Hit
GGGTGAACTTGAGAGCTTCAGTCCATTCTTTCAGGACTTGCACTTCTGCG420.10048568079048736No Hit
ATCAAGATCTATCTCTCTTCGTAGATCAAGATCAGCTTCACTCAGAAGAT420.10048568079048736No Hit
TCACAAATCCCTTATAATAATGTAAACAAAATAACTTTTCCCTAAAGGGT420.10048568079048736No Hit
CTCTTGTGTGGTCCCAGAAGGAGAAGTGAGTTTGCCAAGGATATGGGGCA420.10048568079048736No Hit
GAGTACATGGGAGTGAGGGAAGAGGCCACCAGGTGGCAGCACAGCCACAC420.10048568079048736No Hit
CAGTTACACCCAGCAGAGGGGCACAGGCTTAAACGCCGGCATATTAGTTT420.10048568079048736No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)