FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41797
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGATCACATTCTCGATCCAGAGGAAGGCGATACTCTCGCTCACGCA1910.45697059597578776No Hit
CACATAAACTGCGGGCTGAGTGAGCCTACAGACAATATGAGAAACCAGCA1650.39476517453405746No Hit
CACTTACCCTGTGTTTAAAAAGATCTTGTACCAAGCCAACGGCGTTCCTG1470.3516998827667058No Hit
GTACATGGGAGTGAGGGAAGAGGCCACCAGGTGGCAGCACAGCCACACCC1320.31581213962724597No Hit
GATAAAGACAGCCAGGTCCATGGCCGGGCGCAGTGGCTCATGCCTGTAAT1270.3038495585807594No Hit
GGGTAAGTGTTGGTAGCACGAGGGGCCTCTATTCCACACACAGGAGAGGT1190.28470942890638085No Hit
GTTCAGATTTGTTCATAAAAGATATGTTACAGGAACATTTTAGAAATCAA1160.2775318802784889No Hit
TCATTATGTTGGCCAGGCTGGTCTCGAACTCCTTACCTCAGGTGATCCAC1150.2751393640691916No Hit
GATCTAGACCGTGACCTATTTTTCAAGTTAGAGAAAAAACAAAATGTCAG1120.2679618154412996No Hit
GTCTTTATCATCCCCACAAACATTTTGAAACTGGAATATTTGTCTTCAGA1070.255999234394813No Hit
ATCCAGAGGAAGGCGATACTCTCGCTCACGCAGCAGGAGCAGGGGACGAA1000.23925162092973182No Hit
GTACATGGGAGTAAAAAAAAAATACTCAACAACTAATAGGCCCAGAGTTT950.2272890398832452No Hit
CTATAAAAGGATCGAGGTATTTCCAATCCCCGTCGAGGTCAAGATCAAGA900.21532645883675863No Hit
CCTTATAATAATGTAAACAAAATAACTTTTCCCTAAAGGGTGAACTTGAG890.2129339426274613No Hit
GTCCTGGGTGGAGGGCAGGTGAACACAAGCTGCTGCCGGGGACTGTCCTG890.2129339426274613No Hit
ATCTTACTGCTGTGAATTTACATAGTAATCCAGATCCATTTTGATTAGAT870.20814891020886667No Hit
ATTATAAGGGATTTGTGATGTCTGTAAAGTGTAACCTAGGAAAGATAATT870.20814891020886667No Hit
CCCAAGTACCTGGGACTACAGGCGTGAGCTACCATGCCCGGCTAATTTTT850.20336387779027204No Hit
ACCCAGGACAGTCCCCGGGCTGGCTCCAGGCTGCAGGGGGTCAGCAGAGG820.1961863291623801No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.19140129674378542No Hit
CAATAAAACTTTGTACACAGGAAGTAGCAAAATACATCATTTTTCATAGA800.19140129674378542No Hit
GGACAGGGCTTATCATTTATAGTCTTGTTTCCATTTTCTGAAGACAAATA790.1890087805344881No Hit
GCCCTGTCCCTAGCACCACCTCTCCTGTGTGTGGAATAGAGGCCCCTCGT780.18661626432519082No Hit
GTTTATGTGTGTGCTTTTTTCTATGAAAAATGATGTATTTTGCTACTTCC780.18661626432519082No Hit
CATAAACTGCGGGCTGAGTGAGCCTACAGACAATATGAGAAACCAGCACA760.18183123190659617No Hit
GTTTTGAGATTTGATTTCAGCAGAGGCACACAAACTCTTAAAAACGAGTT740.17704619948800152No Hit
GACCTAGATCTTCTGAGTGAAGCTGATCTTGATCTACGAAGAGAGATAGA730.1746536832787042No Hit
TAGTAATCCAGATCCATTTTGATTAGATGGTTGAATTATCTTTCCTAGGT730.1746536832787042No Hit
ATCTAGGTCTGGTTCTATAAAAGGATCGAGGTATTTCCAATCCCCGTCGA720.17226116706940692No Hit
CAATATGAGAAACCAGCACACGCTTTGGAATACGGTAGGGCAAAACTCCT720.17226116706940692No Hit
CTACTAGGCCCCCTTACTCCTAATTAATTGCCTGCTCACCAGACTGTGAG680.1626911022322176No Hit
CTCCTAAGGAAGCCGAAAATGTTCATCCTGTGGGCAGGAGAGCCAGGAAC680.1626911022322176No Hit
ACTTTGTACACAGGAAGTAGCAAAATACATCATTTTTCATAGAAAAAAGC680.1626911022322176No Hit
GTACAAGATCTTTTTAAACACAGGGTAAGTGTTGGTAGCACGAGGGGCCT680.1626911022322176No Hit
GTCCTGGGTGGACGGCAGGTGAACACAAGCGGCTGCCGCATGTAGCCACT670.1602985860229203No Hit
GTTACACCCAGCAGAGGGGCACAGGCTTAAACGCCGGCATATTAGTTTTC650.15551355360432567No Hit
GTCCCTAGCACCACCTCTCCTGTGTGTGGAATAGAGGCCCCTCGTGCTAC640.15312103739502836No Hit
CCCTAGCACCACCTCTCCTGTGTGTGGAATAGAGGCCCCTCGTGCTACCA640.15312103739502836No Hit
GGAAGTAGCAAAATACATCATTTTTCATAGAAAAAAGCACACACATAAAC640.15312103739502836No Hit
ATTTAAATGTGCCAAATAAAAACCCACATTTTCAGACCATATGATGTTAA630.15072852118573102No Hit
CTTTTATAGAACCAGACCTAGATCTTCTGAGTGAAGCTGATCTTGATCTA630.15072852118573102No Hit
TCACAAATCCCTTATAATAATGTAAACAAAATAACTTTTCCCTAAAGGGT620.1483360049764337No Hit
CTAATAGGCCCAGAGTTTATTTCCAAATGAGACACTAAATTTAAATAGTT610.1459434887671364No Hit
GCATAAAATATTCGGGGGAACCAGCACTTAAAAAACAGACTTCATGAAAG600.14355097255783908No Hit
CTTTGGAATACGGTAGGGCAAAACTCCTAAGGAAGCCGAAAATGTTCATC600.14355097255783908No Hit
ATTTATTTCAGAGCAGTTACACCCAGCAGAGGGGCACAGGCTTAAACGCC600.14355097255783908No Hit
ACCCAGGACAGTCCCCGGCAGCAGCTTGTGTTCACCTGCCCTCCACCCAG600.14355097255783908No Hit
ATAATAATGTAAACAAAATAACTTTTCCCTAAAGGGTGAACTTGAGAGCT590.14115845634854177No Hit
GGCTTAAACGCCGGCATATTAGTTTTCCCGCACGCGAGCCCTGCATGGCG590.14115845634854177No Hit
CCTCTGGATCGAGAATGTGATCTAGACCGTGACCTATTTTTCAAGTTAGA580.13876594013924445No Hit
TTTATAGTCTTGTTTCCATTTTCTGAAGACAAATATTCCAGTTTCAAAAT570.1363734239299471No Hit
GTTTCACTCTGTTGCCCAAGGTAGAGTGCAGTGGCGTGATCTCTGCTCAT570.1363734239299471No Hit
ATCTTGTACCAAGCCAACGGCGTTCCTGGCTCTCCTGCCCACAGGATGAA570.1363734239299471No Hit
ACCATGCCCGGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCATTAT570.1363734239299471No Hit
GCCATGGACCTGGCTGTCTTTATCATCCCCACAAACATTTTGAAACTGGA560.1339809077206498No Hit
ATTCCAAAGCGTGTGCTGGTTTCTCATATTGTCTGTAGGCTCACTCAGCC560.1339809077206498No Hit
GTGCTAGGGACAGGGCTTATCATTTATAGTCTTGTTTCCATTTTCTGAAG560.1339809077206498No Hit
ACAACTAATAGGCCCAGAGTTTATTTCCAAATGAGACACTAAATTTAAAT540.12919587530205517No Hit
ACATAATGAAACCCCATCTCTACTAAAAATACAAAAATTAGCCGGGCATG540.12919587530205517No Hit
ACCGTATTCCAAAGCGTGTGCTGGTTTCTCATATTGTCTGTAGGCTCACT540.12919587530205517No Hit
GTGTTTAAAAAGATCTTGTACCAAGCCAACGGCGTTCCTGGCTCTCCTGC540.12919587530205517No Hit
AGGTAGAGTGCAGTGGCGTGATCTCTGCTCATTGCAACTGCCGCCTCCCG530.12680335909275786No Hit
GTCCATGGCCGGGCGCAGTGGCTCATGCCTGTAATCCCAGCACTTCGGGA530.12680335909275786No Hit
AGGTTACACTTTACAGACATCACAAATCCCTTATAATAATGTAAACAAAA530.12680335909275786No Hit
CCATTATAGTTACTGCACATAAGACTATTACTACTAAAGGTCACTTCAGA530.12680335909275786No Hit
CCTTAGGAGTTTTGCCCTACCGTATTCCAAAGCGTGTGCTGGTTTCTCAT520.12441084288346052No Hit
ATGTTGGCCAGGCTGGTCTCGAACTCCTTACCTCAGGTGATCCACCCACC510.12201832667416322No Hit
CATTTATAGTCTTGTTTCCATTTTCTGAAGACAAATATTCCAGTTTCAAA510.12201832667416322No Hit
ATTTTATGCAGAGGAGGGAAAATTTATAGTGGCAATTATTTTCTCACAGT500.11962581046486591No Hit
GTATCAACGCAGAGTACATGGGAGTGAGGGAAGAGGCCACCAGGTGGCAG500.11962581046486591No Hit
GAATAGAGGCCCCTCGTGCTACCAACACTTACCCTGTGTTTAAAAAGATC500.11962581046486591No Hit
ACCAAGAAGCAGTCGTTCCCCATCAGGAAGTCCTCGCAGAAGTGCAAGTC500.11962581046486591No Hit
GTTCATGCAAAGCACAAAAAACATTTACAATAAAACTTTGTACACAGGAA480.11484077804627127No Hit
GGACACAGAGAGGCCAGGAGCCCCTTCCTGGTTCCCCCTGGTTCCAAATG480.11484077804627127No Hit
TTCTGGGACCACACAAGAGACATGGGAGAATGTTCCTAATTAGGCAAGAG480.11484077804627127No Hit
TCCTGATGGGGAACGACTGCTTCTTGGTCGTGAAATAGACCTGGATCTTG480.11484077804627127No Hit
GAGTACATGGGAGTGAGGGAAGAGGCCACCAGGTGGCAGCACAGCCACAC480.11484077804627127No Hit
GATTTCAGCAGAGGCACACAAACTCTTAAAAACGAGTTATTGTCTGACAT470.11244826183697396No Hit
GCCCAAGGTAGAGTGCAGTGGCGTGATCTCTGCTCATTGCAACTGCCGCC470.11244826183697396No Hit
ATTCCACACACAGGAGAGGTGGTGCTAGGGACAGGGCTTATCATTTATAG470.11244826183697396No Hit
GTACATGGGAGTAAAAAAAAAAATACTCAACAACTAATAGGCCCAGAGTT470.11244826183697396No Hit
TCTCTGCTCATTGCAACTGCCGCCTCCCGGGTTCAAGCAATTCTCATGCA460.11005574562767662No Hit
GTACATGGGAAGGGATGATATTTCTACCACCTCACTCATATTTTTAGCTG460.11005574562767662No Hit
ATCAAGATCTATCTCTCTTCGTAGATCAAGATCAGCTTCACTCAGAAGAT460.11005574562767662No Hit
GAGTACATGGGAGTAAAAAAAAAATACTCAACAACTAATAGGCCCAGAGT460.11005574562767662No Hit
GTATCAACGCAGAGTACATGGGAGTAAAAAAAAAATACTCAACAACTAAT460.11005574562767662No Hit
GATTACAGGCATGAGCCACTGCGCCCGGCCATGGACCTGGCTGTCTTTAT450.10766322941837932No Hit
ACATGGGAGTGAGGGAAGAGGCCACCAGGTGGCAGCACAGCCACACCCGT450.10766322941837932No Hit
ATCTATCTCTCTTCGTAGATCAAGATCAGCTTCACTCAGAAGATCTAGGT450.10766322941837932No Hit
CTCTGTGTCCTCTGCAGTGGGGGTTGTGGGGGGCGCAGCATCCACGCCTT450.10766322941837932No Hit
GAGTGAGCCTACAGACAATATGAGAAACCAGCACACGCTTTGGAATACGG420.10048568079048736No Hit
TTTTAAACACAGGGTAAGTGTTGGTAGCACGAGGGGCCTCTATTCCACAC420.10048568079048736No Hit
CTCTTGTGTGGTCCCAGAAGGAGAAGTGAGTTTGCCAAGGATATGGGGCA420.10048568079048736No Hit
CGTCAGAGGAGGGCAAGGCTGGGTACTCAGCAGCCACTCTGAGCCGGGGC420.10048568079048736No Hit
CTTCTTGGTCGTGAAATAGACCTGGATCTTGATCTTGACCTCGACGGGGA420.10048568079048736No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)