FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66970
Sequences flagged as poor quality0
Sequence length100
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT26864.010751082574287No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT9121.3618037927430193TruSeq Adapter, Index 11 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8041.2005375541287144No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG8001.1945647304763327TruSeq Adapter, Index 11 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6340.9466925489024937No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT6340.9466925489024937RNA PCR Primer, Index 11 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3590.5360609228012543No Hit
ATTTAAAAATTATCATCAGTAAGTTTTCCTTTCTCATGGGTCAGAGAAAA3590.5360609228012543No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA3060.4569210094071972RNA PCR Primer, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGT2760.41212483201433475No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT2690.4016723906226669TruSeq Adapter, Index 11 (96% over 26bp)
ATCTAGTACTGCAACTGCCAGAACTTGGTATTGTAGCTGCTGCCCGCTGA2210.32999850679408693No Hit
GTGTGAGGTTCAGTGATTGTTGACCCTCCCCAGCCCCTCCTGCTTCTTTA2080.3105868299238465No Hit
GCTATAAGCAGTTGACTTTCACCCTTGGAGAGCATCACACTGTGTGAGGT2000.2986411826190832No Hit
CTATAAGAGGTGTGGGTGTCTGTTTGGTCAGGATGTTAGAAAGTGCTGAT1580.2359265342690757No Hit
AAATTACACTATTTAAAAATTATCATCAGTAAGTTTTCCTTTCTCATGGG1570.2344333283559803No Hit
ATATCTAGTACTGCAACTGCCAGAACTTGGTATTGTAGCTGCTGCCCGCT1490.22248768105121697No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG1450.2165148573988353TruSeq Adapter, Index 11 (95% over 24bp)
CATTTACACAATAGGCTCCCGGCAGCATTTCCAGACAAATGTTCTTTTAA1310.19560997461549948No Hit
GCTTATAGCCATTTCAAAAGTATTTTAAAGAAAAATGTTTTGTAGGGAAA1310.19560997461549948No Hit
ATAACATACATTACAAAAATAAGTTTGACTGTTTCAACTACACGGTTATG1100.16425265044049575No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGT1080.16126623861430492No Hit
ACCTAATTGCATTCAAAACCATTTTCAAATCCATCTTTAAACTAGTCAGA1060.1582798267881141No Hit
GCATCACACTGTGTGAGGTTCAGTGATTGTTGACCCTCCCCAGCCCCTCC1050.15678662087501866No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTT980.14633417948335076No Hit
ACTTAACACTGAACAGATAAGACCTCTTAAAAGGCAGCTGACTATATCAT960.14334776765715992No Hit
GTACATGGGGATTGATTGATTGATTGATAGAAAGAAAGTTGCTTTTCTTT960.14334776765715992No Hit
GTATCAACGCAGAGTACTTTCATTTTTTTCTTTTTTTTTTTTTTTTTTTT900.13438853217858743No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.13438853217858743No Hit
CTATAAATGAATAAATTACACTATTTAAAAATTATCATCAGTAAGTTTTC890.132895326265492No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTCTTTTCTTTTTCTTTTTTTTTT880.1314021203523966No Hit
ACCTCACACAGTGTGATGCTCTCCAAGGGTGAAAGTCAACTGCTTATAGC870.1299089144393012No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.12841570852620576No Hit
GTGTGATGCTCTCCAAGGGTGAAAGTCAACTGCTTATAGCCATTTCAAAA840.12542929670001493No Hit
GTATTGTAGCTGCTGCCCGCTGACTAGCAGCTGGACTGATTTTGAATAAA840.12542929670001493No Hit
GTCCTGCCTCAGCCTCCGGAGTAGCTGGGACCACAGGTTCATGCCACCAT840.12542929670001493No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGT820.1224428848738241No Hit
CATTAAAGGGTTTCCCTACAAAACATTTTTCTTTAAAATACTTTTGAAAT820.1224428848738241No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.1224428848738241No Hit
GCATTAAAGGGTTTCCCTACAAAACATTTTTCTTTAAAATACTTTTGAAA810.1209496789607287No Hit
GATAAGACCTCTTAAAAGGCAGCTGACTATATCATGTCACCATCATAGCC800.11945647304763327No Hit
GGCTATAAGCAGTTGACTTTCACCCTTGGAGAGCATCACACTGTGTGAGG800.11945647304763327No Hit
GTATCAACGCAGAGTAGCTGGGATTACAGGCACCCGCCACCACGCCTGGC800.11945647304763327No Hit
GTACATGGGAATTCAAGATGAGATTGCCGTCAAATTGTCAGTGGAAATAC790.11796326713453785No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT790.11796326713453785No Hit
GGTCAGAGAAAAAGAAATGAGCGTGCAAAGAAGATTGAGAGGAAGCGCAC780.11647006122144243No Hit
GGTATAAGATGGTCAATTTATCACGCCTGTAATCCCAGCACTTTGGGATG780.11647006122144243No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC770.11497685530834703No Hit
TCAGTAAGTTTTCCTTTCTCATGGGTCAGAGAAAAAGAAATGAGCGTGCA760.1134836493952516No Hit
GACTATATCATGTCACCATCATAGCCAATACAACATTTTTGCCATACTTC760.1134836493952516No Hit
GTTTATGATACTACTAATTCAAGGCTACTTGGAGCTTTCTTTACAGTTCA750.11199044348215619No Hit
TCTTATACCAGGAAGTCAAATGAGAAGACATCAGTAAAAATATTCTGGCC740.11049723756906078No Hit
TCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTTCTGCTTG740.11049723756906078Illumina PCR Primer Index 9 (96% over 26bp)
ACATAATACATGTTCATATACAAACATTTAAACACATACACACAAAGATC700.1045244139166791No Hit
GTGTATGCGAAAAGGTTTTTAGGAAGTATGGCAAAAATGTTGTATTGGCT700.1045244139166791No Hit
GGTATCAACGCAGAGTACTTTCATTTTTTTCTTTTTTTTTTTTTTTTTTT690.10303120800358369No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC690.10303120800358369RNA PCR Primer, Index 17 (95% over 24bp)
ATGTAAAAGATGTTGACCCTTCCAGCTGGACGTGGTGGCTCACAATTGTA690.10303120800358369No Hit
TCTTTAAACTAGTCAGAAAACAGGTTATTATTTTTTTAAATCACTTAACA690.10303120800358369No Hit
GTATTAGCTTTGAAATTGAATAAAGAAAGCTGTTTGTAGTTTTAAAATGC690.10303120800358369No Hit
GTACATGGCTCTGGTTCTGGACACAAAATCTGTACTGGAGAGGAAATGAC690.10303120800358369No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTTCT680.10153800209048829Illumina PCR Primer Index 9 (95% over 21bp)
ATATAAAAATGGGATATAAAAAGGGAGAAGGAGGGGAAGGGTGGGGTGAA670.10004479617739286No Hit
CAATAACATACATTACAAAAATAAGTTTGACTGTTTCAACTACACGGTTA670.10004479617739286No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.10004479617739286Clontech SMART CDS Primer II A (100% over 21bp)
GAACAGATAAGACCTCTTAAAAGGCAGCTGACTATATCATGTCACCATCA670.10004479617739286No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)