FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66970
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAAAAATTATCATCAGTAAGTTTTCCTTTCTCATGGGTCAGAGAAAA3580.5345677168881589No Hit
ATCTAGTACTGCAACTGCCAGAACTTGGTATTGTAGCTGCTGCCCGCTGA3200.47782589219053306No Hit
GTGTGAGGTTCAGTGATTGTTGACCCTCCCCAGCCCCTCCTGCTTCTTTA2190.32701209496789607No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910.2852023294012244No Hit
GCTATAAGCAGTTGACTTTCACCCTTGGAGAGCATCACACTGTGTGAGGT1830.2732566820964611No Hit
GCTTATAGCCATTTCAAAAGTATTTTAAAGAAAAATGTTTTGTAGGGAAA1670.24936538748693446No Hit
CTATAAGAGGTGTGGGTGTCTGTTTGGTCAGGATGTTAGAAAGTGCTGAT1570.2344333283559803No Hit
ATATCTAGTACTGCAACTGCCAGAACTTGGTATTGTAGCTGCTGCCCGCT1520.2269672987905032No Hit
GTACATGGGGATTGATTGATTGATTGATAGAAAGAAAGTTGCTTTTCTTT1310.19560997461549948No Hit
CATTTACACAATAGGCTCCCGGCAGCATTTCCAGACAAATGTTCTTTTAA1290.19262356278930864No Hit
ACCTCACACAGTGTGATGCTCTCCAAGGGTGAAAGTCAACTGCTTATAGC1200.1791847095714499No Hit
ATAACATACATTACAAAAATAAGTTTGACTGTTTCAACTACACGGTTATG1200.1791847095714499No Hit
CATTAAAGGGTTTCCCTACAAAACATTTTTCTTTAAAATACTTTTGAAAT1190.17769150365835448No Hit
AAATTACACTATTTAAAAATTATCATCAGTAAGTTTTCCTTTCTCATGGG1160.17321188591906822No Hit
ACCTAATTGCATTCAAAACCATTTTCAAATCCATCTTTAAACTAGTCAGA1110.16574585635359115No Hit
GTATTGTAGCTGCTGCCCGCTGACTAGCAGCTGGACTGATTTTGAATAAA1110.16574585635359115No Hit
GTACATGGGAATTCAAGATGAGATTGCCGTCAAATTGTCAGTGGAAATAC1070.15977303270120952No Hit
GATAAGACCTCTTAAAAGGCAGCTGACTATATCATGTCACCATCATAGCC1040.15529341496192325No Hit
GTGTGATGCTCTCCAAGGGTGAAAGTCAACTGCTTATAGCCATTTCAAAA1020.15230700313573242No Hit
ACTTAACACTGAACAGATAAGACCTCTTAAAAGGCAGCTGACTATATCAT960.14334776765715992No Hit
TCTTTAAACTAGTCAGAAAACAGGTTATTATTTTTTTAAATCACTTAACA910.13588173809168286No Hit
ACATAATACATGTTCATATACAAACATTTAAACACATACACACAAAGATC900.13438853217858743No Hit
GACTATATCATGTCACCATCATAGCCAATACAACATTTTTGCCATACTTC870.1299089144393012No Hit
TCTTATACCAGGAAGTCAAATGAGAAGACATCAGTAAAAATATTCTGGCC860.12841570852620576No Hit
CTATAAATGAATAAATTACACTATTTAAAAATTATCATCAGTAAGTTTTC860.12841570852620576No Hit
GCATCACACTGTGTGAGGTTCAGTGATTGTTGACCCTCCCCAGCCCCTCC850.12692250261311036No Hit
GGCTATAAGCAGTTGACTTTCACCCTTGGAGAGCATCACACTGTGTGAGG840.12542929670001493No Hit
GCATTAAAGGGTTTCCCTACAAAACATTTTTCTTTAAAATACTTTTGAAA840.12542929670001493No Hit
GTTTATGATACTACTAATTCAAGGCTACTTGGAGCTTTCTTTACAGTTCA830.12393609078691951No Hit
GTCCTGCCTCAGCCTCCGGAGTAGCTGGGACCACAGGTTCATGCCACCAT830.12393609078691951No Hit
GTATCAACGCAGAGTAGCTGGGATTACAGGCACCCGCCACCACGCCTGGC830.12393609078691951No Hit
GTGTAATTTATTCATTTATAGCATGTCAGGATAAATTAAAAGAACATTTG810.1209496789607287No Hit
TCTTTAAGTTATCTGTGTGCGTGCGCTTCCTCTCAATCTTCTTTGCACGC810.1209496789607287No Hit
CTTTGAGACTGGGTCTCGCTTTGTTGCCCAGGCTGGAGTGGAGTGGCGTG800.11945647304763327No Hit
CCCCAGCCCCTCCTGCTTCTTTAAGTTATCTGTGTGCGTGCGCTTCCTCT790.11796326713453785No Hit
ATATAAAAATGGGATATAAAAAGGGAGAAGGAGGGGAAGGGTGGGGTGAA750.11199044348215619No Hit
GGTATAAGATGGTCAATTTATCACGCCTGTAATCCCAGCACTTTGGGATG750.11199044348215619No Hit
CTCCAAGGGTGAAAGTCAACTGCTTATAGCCATTTCAAAAGTATTTTAAA730.10900403165596535No Hit
GTATCAACGCAGAGTACTCTTTTTCTTTTTTTTTCCTTTTTTGTCTATGA730.10900403165596535No Hit
CTTAAAAGGCAGCTGACTATATCATGTCACCATCATAGCCAATACAACAT730.10900403165596535No Hit
GACTAGCAGCTGGACTGATTTTGAATAAAAATGAAAGCATTAAAGGGTTT700.1045244139166791No Hit
CATTTATAGCATGTCAGGATAAATTAAAAGAACATTTGTCTGGAAATGCT700.1045244139166791No Hit
GAACAGATAAGACCTCTTAAAAGGCAGCTGACTATATCATGTCACCATCA690.10303120800358369No Hit
CTCTATAAGAGGTGTGGGTGTCTGTTTGGTCAGGATGTTAGAAAGTGCTG690.10303120800358369No Hit
GTATAAGATGGTCAATTTATCACGCCTGTAATCCCAGCACTTTGGGATGC680.10153800209048829No Hit
ACACTGAACAGATAAGACCTCTTAAAAGGCAGCTGACTATATCATGTCAC670.10004479617739286No Hit
ACGCAGAGTAGCTGGGATTACAGGCACCCGCCACCACGCCTGGCTAATTT670.10004479617739286No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)