FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41320
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT990323.966602129719263No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT39779.624878993223621RNA PCR Primer, Index 26 (100% over 22bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG31127.531461761858664RNA PCR Primer, Index 26 (100% over 29bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT27766.718296224588577RNA PCR Primer, Index 26 (100% over 24bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT13653.3034849951597294RNA PCR Primer, Index 26 (100% over 27bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA10572.558083252662149RNA PCR Primer, Index 26 (100% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGT7921.9167473378509197No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG7661.8538238141335914RNA PCR Primer, Index 26 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC4040.9777347531461762RNA PCR Primer, Index 26 (95% over 22bp)
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC3950.9559535333978704RNA PCR Primer, Index 26 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGT3730.9027105517909003No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT3150.7623426911907067RNA PCR Primer, Index 26 (100% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC3100.7502420135527589No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTT2910.7042594385285577RNA PCR Primer, Index 26 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGT2560.6195546950629236No Hit
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC1830.4428848015488867No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCT1740.42110358180058083RNA PCR Primer, Index 26 (95% over 24bp)
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT1400.3388189738625363RNA PCR Primer, Index 26 (96% over 30bp)
CTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC1270.3073572120038722RNA PCR Primer, Index 26 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGTCTT1250.30251694094869314RNA PCR Primer, Index 26 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.28799612778315586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.2686350435624395No Hit
TCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCTGCTTG1090.2637947725072604RNA PCR Primer, Index 26 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGTCTTCT950.22991287512100678RNA PCR Primer, Index 26 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATATCGTATGCCGT890.2153920619554695No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGAG790.19119070667957405No Hit
TCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGTCTTCTGCTTG790.19119070667957405RNA PCR Primer, Index 26 (96% over 29bp)
CATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCTGCT720.17424975798644723RNA PCR Primer, Index 26 (96% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.16456921587608905No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTTAGCATCTCGTATGCCGT600.14520813165537272No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGA600.14520813165537272No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGTCTT550.13310745401742496RNA PCR Primer, Index 26 (95% over 22bp)
CTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCTGCTTGA540.13068731848983542RNA PCR Primer, Index 26 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGTCTTCT480.11616650532429815RNA PCR Primer, Index 26 (95% over 24bp)
TCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAA470.1137463697967086RNA PCR Primer, Index 26 (96% over 31bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACTCCTGAG430.10406582768635045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)