FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174159
Sequences flagged as poor quality0
Sequence length100
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT59913.439960036518354No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT20311.1661757359654108RNA PCR Primer, Index 27 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18441.0588025884392998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13820.7935277533747898No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT13730.7883600617826239RNA PCR Primer, Index 27 (96% over 25bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG11230.6448130731113523RNA PCR Primer, Index 27 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8670.4978209567119701No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGT5400.3100614955299468No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT5270.30259705211904064RNA PCR Primer, Index 27 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3900.22393330232718375No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA3350.192352964819504RNA PCR Primer, Index 27 (96% over 30bp)
GTATAGACAAGCTTTGTAGTGAAGTATAGTAGCAATAATTTCTGTACCTG2780.15962425140245406No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG2760.15847587549308392RNA PCR Primer, Index 27 (100% over 24bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2740.15732749958371373No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2430.13952767298847604No Hit
GTACATGAGAATCCTTTTTTGACAAGCCTTGGAAAGCTGACACTGTCTCT2270.13034066571351466No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2230.12804391389477432Clontech SMART CDS Primer II A (100% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC2200.12632135003071907No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGT2160.1240245982119787No Hit
GTATTAACAAATGGCAATGAGTAGAAAAGTTAACATGAAGATTTTAGAAG2080.11943109457449802No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.11081827525422172No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGC1890.10852152343548135TruSeq Adapter, Index 27 (96% over 25bp)
GTATTAGGTTAGGTCACAAAGGTTTTATCTGAGGTGATTTAAATAACTTC1860.10679895957142613No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.10565058366205594No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC1820.10450220775268576No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT1780.10220545593394542RNA PCR Primer, Index 27 (95% over 22bp)
AAGTAATACAATGTGCCAATCCATAAAATGTAAGTTAACAGGTTTCTCAA1760.10105708002457524No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)