FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174159
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9000.516769159216578No Hit
GTATAGACAAGCTTTGTAGTGAAGTATAGTAGCAATAATTTCTGTACCTG2810.16134681526650932No Hit
GTACATGAGAATCCTTTTTTGACAAGCCTTGGAAAGCTGACACTGTCTCT2510.14412117662595675No Hit
GTATTAACAAATGGCAATGAGTAGAAAAGTTAACATGAAGATTTTAGAAG2340.13435998139631028No Hit
AAGTAATACAATGTGCCAATCCATAAAATGTAAGTTAACAGGTTTCTCAA2030.11656015480107258No Hit
CTATAAGAGGTGTGGGTGTCTGTTTGGTCAGGATGTTAGAAAGTGCTGAT1990.11426340298233224No Hit
GTCTACAAGGTCCCACTTCTTGTCTGTCTCCAAATTCACTTTTGAGGATC1890.10852152343548135No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)