FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85879
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT876010.200398234725602No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT33403.889192934244693RNA PCR Primer, Index 39 (95% over 23bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG33043.8472734894444507RNA PCR Primer, Index 39 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT23072.686337754282188RNA PCR Primer, Index 39 (96% over 25bp)
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA13131.5288953061866113RNA PCR Primer, Index 39 (96% over 30bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT11611.351902094807811RNA PCR Primer, Index 39 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7020.8174291736047229No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG5670.6602312556038147Illumina PCR Primer Index 11 (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5410.629956101025862No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACAACTCGTATGCCGT5190.6043386625368251No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGC3560.41453673191350615Illumina PCR Primer Index 11 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC3250.3784394322244088No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.3469998486242271No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT2670.31090254893512964RNA PCR Primer, Index 39 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACAGCTCGTATGCCGT2500.29110725555723754No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC2370.2759696782682612No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACACCTCGTATGCCGT1570.18281535648994515No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC1480.1723354952898846RNA PCR Primer, Index 39 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACAACTCGTATGCCGTCTT1470.17117106626765566TruSeq Adapter, Index 11 (95% over 21bp)
TCTCCGAGCCCACGAGACCTCTCTACAACTCGTATGCCGTCTTCTGCTTG1360.1583623470231372TruSeq Adapter, Index 11 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.1560334889786793No Hit
ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTT1230.14322476973416085Illumina PCR Primer Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACAACTCGTATGCCGTCTTCT1200.139731482667474RNA PCR Primer, Index 11 (95% over 23bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.139731482667474No Hit
CTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC1190.13856705364524505No Hit
CATATATGTTGTGTATAAATGTACACTTTTTTAGACATGAAAGAATTGCC1090.12692276342295555No Hit
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAA1070.12459390537849767Illumina PCR Primer Index 11 (96% over 29bp)
GGGTATAACTGGGTAATACTCAGTTGAGTAATGTAGTTTACTGTGAGTGC1040.1211006183118108No Hit
GTACATGGGGTGGCTGATCATAAGAAAGGAGCAAAGAAAGCTAGAATTGA990.11527847320066606No Hit
GTGTTGGCATTTGCACTGGTGATGCTAAAGTAATGGTGGATAAGCTGCTG960.1117851861339792No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.10829189906729235No Hit
GTACATGGGACTCTAAAAGCTGCAGATGAACAGATGGCTTTGTCAAAAGT880.1024697539561476No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACAGCTCGTATGCCGTCTT870.10130532493391865TruSeq Adapter, Index 11 (95% over 21bp)
GATTGATACAGTGTGTAATGATCAAATCAGGTTAATTAGGGTATCCATCA860.1001408959116897No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)