FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39708
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT1077027.12299788455727No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT37579.461569457036365RNA PCR Primer, Index 31 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT26476.666162989825727RNA PCR Primer, Index 31 (95% over 24bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG12433.1303515664349755RNA PCR Primer, Index 31 (96% over 29bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT10612.6720056411806183RNA PCR Primer, Index 31 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGT8262.0801853530774657No Hit
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG6061.5261408280447264RNA PCR Primer, Index 38 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTAGCTCGTATGCCGT3970.9997985292636244No Hit
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC3640.9166918505087137No Hit
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT3400.8562506295960512RNA PCR Primer, Index 31 (95% over 21bp)
ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC3240.8159564823209429RNA PCR Primer, Index 38 (96% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCC3010.7580336456129747No Hit
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA2670.6724085826533696RNA PCR Primer, Index 31 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGTCTT2540.6396695879923442No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTACCTCGTATGCCGT2430.6119673617407072No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2430.6119673617407072No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.4759746146872167No Hit
CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC1870.4709378462778281No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGTCTTCT1820.45834592525435686RNA PCR Primer, Index 38 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCCATCTCGTATGCCGT1450.3651657096806689No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.27954064672106377No Hit
CTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC1040.2619119572882039No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTAGCTCGTATGCCGTCTT1000.25183842046942684No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.24428326785534402No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTAGCTCGTATGCCGTCTTCT970.24428326785534402RNA PCR Primer, Index 38 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.23420973103656695No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCTACGC810.20398912058023572No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATATCGTATGCCGT730.18384204694268158No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTACCTCGTATGCCGTCTT700.17628689432859876No Hit
CATCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGTCTTCTGCT620.15613982069104462RNA PCR Primer, Index 38 (96% over 25bp)
ATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTT600.1511030522816561RNA PCR Primer, Index 38 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGCTACGCCATCTCGTATGCCGTCTT550.13851113125818473RNA PCR Primer, Index 41 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCCT460.11584567341593632No Hit
CACATCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGTCTTCTG450.11332728921124206RNA PCR Primer, Index 27 (95% over 22bp)
CTTATCACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC440.1108089050065478No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCTACG420.10577213659715926No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTACCTCGTATGCCGTCTTCT410.103253752392465RNA PCR Primer, Index 38 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCCTCT410.103253752392465RNA PCR Primer, Index 38 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)