FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183786
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25301.376601046869729No Hit
TTATAATACAGACTTAAATGTACAAAGATGTTTTCCACTTTTTTCAATTT3620.19696821303037226No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3560.1937035465160567No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3190.17357143634444408No Hit
GTCCAGAGGCTTTAAAACTGGTGCAATTACAGCAAAAAGGGATTCTGTAG2680.1458217709727618No Hit
GTATTATAATAAGCAAAAAGATTGTGTGTATGTATGTTTAATATAACATG2390.13004254948690325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.12732199405830694No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACATC2310.12568966080114916No Hit
GTATATATCAACAATCTGACAGGCAGTGAACTTGACATGATTAGCTGGCA2260.12296910537255286No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT2220.12079266102967583No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG2130.11589566125820247No Hit
ACATATAGATCATTTATTTTCCTTCTAGTCCCGGGTTCAGTACATAGATG2020.10991043931529061No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT1960.10664577280097504No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG1950.10610166171525579No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT1910.10392521737237874No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)