FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41498
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT20985.055665333269073No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT7041.6964672996288979TruSeq Adapter, Index 11 (95% over 21bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG6061.460311340305557TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT4851.16873102318184RNA PCR Primer, Index 11 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.6000289170562436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.5927996529953251No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA2340.5638825967516508RNA PCR Primer, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGT2020.486770446768519No Hit
GTCCAGGATTGTGAAGTACAACACATTTTAAGGATGAGACTTTCCTTTCA1920.4626728998987903No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT1820.4385753530290616TruSeq Adapter, Index 11 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.2867608077497711No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG1140.27471203431490676TruSeq Adapter, Index 11 (95% over 24bp)
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT1140.27471203431490676No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG1110.26748277025398814No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG1040.25061448744517806No Hit
GGGTAGAGGAGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT1020.24579497807123235No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT980.23615595932334085No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACATC960.23133644994939515No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA950.2289266952624223No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGT950.2289266952624223No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC920.22169743120150368No Hit
GTACATGGGCTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACAC910.2192876765145308No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC880.21205841245361223No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT880.21205841245361223No Hit
GCTCTAGAGGGGGTAGAGGAGGTGCTATAGGGTAAATACGGGCCCTATTT870.20964865776663938No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA860.20723890307966647No Hit
GTATGAAACTGTGGTTTGCTCCACAGATTTCAGAGCATTGACCGTAGTAT800.1927803749578293No Hit
TGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATAC790.19037062027085644No Hit
GTAACAAGACTGGCTGGCTGATGAGCAAAGCCTTTGCTCTCACGCAGAGG770.1855511108969107No Hit
GTACATGGGGAACAATGAAGAGGCCTGCGCCTCCTGCCTTGCCGCCTGCA770.1855511108969107No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC730.17591209214901923No Hit
GCATCAGGTTATTTAAAACAACACGCCTGTGGGACCCCGTTCCTGGAGGA720.17350233746204635No Hit
GTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTA700.16868282808810064No Hit
AACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTA700.16868282808810064No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC700.16868282808810064RNA PCR Primer, Index 17 (95% over 24bp)
TCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAG700.16868282808810064No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC700.16868282808810064No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC690.16627307340112776No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC690.16627307340112776No Hit
GTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCT680.1638633187141549No Hit
GTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCG670.16145356402718206No Hit
TCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTAC640.15422429996626344No Hit
GTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGCTTCAATCG630.15181454527929056No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTT630.15181454527929056No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.1494047905923177Clontech SMART CDS Primer II A (100% over 21bp)
TGCCTGTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTTGATTG600.14458528121837197No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC600.14458528121837197No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTTCT590.14217552653139912Illumina PCR Primer Index 9 (95% over 21bp)
GCAAAGAAACTGCCTTTTATTTTTTAACCTTAAAAAGTAGCCAGATAGTA580.13976577184442623No Hit
ATTTAAAACAACACGCCTGTGGGACCCCGTTCCTGGAGGAAGACCCGCTT580.13976577184442623No Hit
GTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTTGATTGTAGAT580.13976577184442623No Hit
TCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCA570.13735601715745338No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA570.13735601715745338No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGT560.1349462624704805No Hit
TTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTG550.13253650778350762No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCAGAGGAG550.13253650778350762No Hit
ATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTTCA550.13253650778350762No Hit
TCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC550.13253650778350762No Hit
GAGGAAGGCTTGGATGTACAATGAAACTGCCTGGAACTAAAAGCAGTGAA550.13253650778350762No Hit
CATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCA550.13253650778350762No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC540.13012675309653476No Hit
GTTATGTAAAGGATGCGTAGGGATGGGAGGGCGATGAGGACTAGGATGAT540.13012675309653476No Hit
GTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTT540.13012675309653476No Hit
ATGTAAAGGATGCGTAGGGATGGGAGGGCGATGAGGACTAGGATGATGGC530.1277169984095619No Hit
GGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCCTAATGTGGGGACA520.12530724372258903No Hit
TTATTATACGAATGGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACG510.12289748903561618No Hit
TTTTAAGGATGAGACTTTCCTTTCATGGTCAAGCACCAGCATCATGCACA500.12048773434864331No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT500.12048773434864331No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT500.12048773434864331No Hit
CTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCC480.11566822497469757No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACATCCCCTATCATAG470.11325847028772472No Hit
TAACAAGACTGGCTGGCTGATGAGCAAAGCCTTTGCTCTCACGCAGAGGA470.11325847028772472No Hit
GATAGTAACAAGACTGGCTGGCTGATGAGCAAAGCCTTTGCTCTCACGCA470.11325847028772472No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA470.11325847028772472No Hit
TAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTGGCCAC470.11325847028772472No Hit
ATGAAGAGGCCTGCGCCTCCTGCCTTGCCGCCTGCAAAGCAAAGAAACTG470.11325847028772472No Hit
GCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAAT470.11325847028772472No Hit
GAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTA460.11084871560075184No Hit
GTATCAACGCAGAGTACATGGGATGGCACATGCAGCGCAAGTAGGTCTAC460.11084871560075184No Hit
GTATCAACGCAGAGTACATGGGCTACCTAAAAAATCCCAAACATATAACT460.11084871560075184No Hit
CGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCC450.10843896091377897No Hit
GTATCAACGCAGAGTACATGGGGAACAATGAAGAGGCCTGCGCCTCCTGC450.10843896091377897No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.10843896091377897No Hit
GGCTTATGCAGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC450.10843896091377897No Hit
CAACAAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACC450.10843896091377897No Hit
CCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCA440.10602920622680612No Hit
GTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAAT440.10602920622680612No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA430.10361945153983323No Hit
GAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGG430.10361945153983323No Hit
CTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCT430.10361945153983323No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.10361945153983323No Hit
GGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTA430.10361945153983323No Hit
GTGTACTCGTAGGTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAG430.10361945153983323No Hit
TTCTAGGACGATGGGTATGAAACTGTGGTTTGCTCCACAGATTTCAGAGC430.10361945153983323No Hit
ATTCTAGGACGATGGGTATGAAACTGTGGTTTGCTCCACAGATTTCAGAG420.10120969685286038No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC420.10120969685286038No Hit
GAGTACATGGGCTACCTAAAAAATCCCAAACATATAACTGAACTCCTCAC420.10120969685286038No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)