FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41498
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGATTGTGAAGTACAACACATTTTAAGGATGAGACTTTCCTTTCA2010.4843606920815461No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.3349559014892284No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT1260.3036290905585811No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACATC1170.2819412983758254No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT1170.2819412983758254No Hit
GTACATGGGCTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACAC1140.27471203431490676No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT1130.27230227962793385No Hit
GGGTAGAGGAGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT1120.269892524940961No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC1120.269892524940961No Hit
GCTCTAGAGGGGGTAGAGGAGGTGCTATAGGGTAAATACGGGCCCTATTT1080.26025350619306953No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG1070.2578437515060967No Hit
GTATGAAACTGTGGTTTGCTCCACAGATTTCAGAGCATTGACCGTAGTAT1030.2482047327582052No Hit
GTAACAAGACTGGCTGGCTGATGAGCAAAGCCTTTGCTCTCACGCAGAGG1020.24579497807123235No Hit
TGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATAC1000.24097546869728662No Hit
GTACATGGGGAACAATGAAGAGGCCTGCGCCTCCTGCCTTGCCGCCTGCA980.23615595932334085No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG960.23133644994939515No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC960.23133644994939515No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA930.22410718588847656No Hit
GTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCG930.22410718588847656No Hit
GTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTTGATTGTAGAT900.21687792182755794No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC800.1927803749578293No Hit
TCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAG760.18314135620993785No Hit
TTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTG750.18073160152296497No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC740.1783218468359921No Hit
GTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTA730.17591209214901923No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA730.17591209214901923No Hit
GCAAAGAAACTGCCTTTTATTTTTTAACCTTAAAAAGTAGCCAGATAGTA730.17591209214901923No Hit
TCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCA720.17350233746204635No Hit
GTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTT700.16868282808810064No Hit
GTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCT680.1638633187141549No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC680.1638633187141549No Hit
GCATCAGGTTATTTAAAACAACACGCCTGTGGGACCCCGTTCCTGGAGGA680.1638633187141549No Hit
ATTTAAAACAACACGCCTGTGGGACCCCGTTCCTGGAGGAAGACCCGCTT670.16145356402718206No Hit
ATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTTCA670.16145356402718206No Hit
TCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTAC650.1566340546532363No Hit
GTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGCTTCAATCG640.15422429996626344No Hit
CATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCA640.15422429996626344No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA620.1494047905923177No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC600.14458528121837197No Hit
GAGGAAGGCTTGGATGTACAATGAAACTGCCTGGAACTAAAAGCAGTGAA590.14217552653139912No Hit
TGCCTGTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTTGATTG580.13976577184442623No Hit
AACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTA560.1349462624704805No Hit
GTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAAT560.1349462624704805No Hit
ATGTAAAGGATGCGTAGGGATGGGAGGGCGATGAGGACTAGGATGATGGC550.13253650778350762No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA530.1277169984095619No Hit
CTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCT530.1277169984095619No Hit
GTTATGTAAAGGATGCGTAGGGATGGGAGGGCGATGAGGACTAGGATGAT520.12530724372258903No Hit
CAACAAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACC520.12530724372258903No Hit
TCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC520.12530724372258903No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT500.12048773434864331No Hit
GTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCT500.12048773434864331No Hit
GTTTTTAAGCCTAATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGA500.12048773434864331No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA490.11807797966167043No Hit
GTGTACTCGTAGGTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAG490.11807797966167043No Hit
TTATTATACGAATGGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACG470.11325847028772472No Hit
GTATCAACGCAGAGTACATGGGGAACAATGAAGAGGCCTGCGCCTCCTGC470.11325847028772472No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC470.11325847028772472No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACATCCCCTATCATAG460.11084871560075184No Hit
CTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCC460.11084871560075184No Hit
GAGTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACA460.11084871560075184No Hit
CTCCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGT460.11084871560075184No Hit
GGGTATGAAACTGTGGTTTGCTCCACAGATTTCAGAGCATTGACCGTAGT460.11084871560075184No Hit
GAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTA450.10843896091377897No Hit
GTATCAACGCAGAGTACATGGGATGGCACATGCAGCGCAAGTAGGTCTAC450.10843896091377897No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCAGAGGAG450.10843896091377897No Hit
GAGCATGCCTGTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTT450.10843896091377897No Hit
GTATCAACGCAGAGTACATGGGCTACCTAAAAAATCCCAAACATATAACT450.10843896091377897No Hit
GGCTTATGCAGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC450.10843896091377897No Hit
ATGAAGAGGCCTGCGCCTCCTGCCTTGCCGCCTGCAAAGCAAAGAAACTG450.10843896091377897No Hit
TAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTGGCCAC440.10602920622680612No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT430.10361945153983323No Hit
TTTTAAGGATGAGACTTTCCTTTCATGGTCAAGCACCAGCATCATGCACA430.10361945153983323No Hit
GAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGG430.10361945153983323No Hit
GGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCCTAATGTGGGGACA420.10120969685286038No Hit
GCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAAT420.10120969685286038No Hit
GGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGA420.10120969685286038No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)