FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77165
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT33904.393183438087216No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT11701.5162314520831983RNA PCR Primer, Index 17 (95% over 21bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG8391.0872805028186354RNA PCR Primer, Index 17 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT8371.0846886541825957RNA PCR Primer, Index 17 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8131.05358647055012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7400.9589839953346724No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAACTCGTATGCCGT5330.6907276615045681No Hit
CTATAAGAGGTGTGGGTGTCTGTTTGGTCAGGATGTTAGAAAGTGCTGAT3630.47042052744119744No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT3240.4198794790384241RNA PCR Primer, Index 17 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3040.3939609926780276No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA2570.3330525497310957RNA PCR Primer, Index 17 (96% over 28bp)
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG2380.30842998768871893No Hit
ACCTAATTGCATTCAAAACCATTTTCAAATCCATCTTTAAACTAGTCAGA2370.30713406337069915No Hit
GATAAGACCTCTTAAAAGGCAGCTGACTATATCATGTCACCATCATAGCC2350.3045422147346595No Hit
TCTTTAAACTAGTCAGAAAACAGGTTATTATTTTTTTAAATCACTTAACA2250.2915829715544612No Hit
ACTTAACACTGAACAGATAAGACCTCTTAAAAGGCAGCTGACTATATCAT2180.2825115013283224No Hit
CTTTAATAGCAAGCGAATGGTAATTACATGGTCGGATGAGGTCCTCACTC2090.270848182466144No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG2020.2617767122400052TruSeq Adapter, Index 12 (95% over 23bp)
GACTATATCATGTCACCATCATAGCCAATACAACATTTTTGCCATACTTC1830.23715415019762848No Hit
GTGTATGCGAAAAGGTTTTTAGGAAGTATGGCAAAAATGTTGTATTGGCT1730.22419490701743017No Hit
GTACATGGGCAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACATAT1700.2203071340633707No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAGCTCGTATGCCGT1690.21901120974535085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1660.21512343679129137No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA1630.2112356638372319No Hit
ATCATAGCCAATACAACATTTTTGCCATACTTCCTAAAAACCTTTTCGCA1620.20993973951921205No Hit
CTCTATAAGAGGTGTGGGTGTCTGTTTGGTCAGGATGTTAGAAAGTGCTG1600.20734789088317243No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAAACTCGTATGCCGTCTT1540.19957234497505347No Hit
GAACAGATAAGACCTCTTAAAAGGCAGCTGACTATATCATGTCACCATCA1490.19309272338495434No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1460.18920495043089483No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1420.1840212531588155No Hit
ACACTGAACAGATAAGACCTCTTAAAAGGCAGCTGACTATATCATGTCAC1410.1827253288407957No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.18142940452277587Clontech SMART CDS Primer II A (100% over 21bp)
CTTAAAAGGCAGCTGACTATATCATGTCACCATCATAGCCAATACAACAT1400.18142940452277587No Hit
GTACTCTATAAGAGGTGTGGGTGTCTGTTTGGTCAGGATGTTAGAAAGTG1330.1723579342966371No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGC1280.16587831270653794TruSeq Adapter, Index 25 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG1220.15810276679841898No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC1200.1555109181623793No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG1180.15291906952633966No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC1110.14384759930020088No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT1070.13866390202812157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1070.13866390202812157No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAAACTCGTATGCCGTCTTCT1050.1360720533920819RNA PCR Primer, Index 19 (95% over 21bp)
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT1030.13348020475604225No Hit
GTATCAACGCAGAGTACTTTTTTTTTCTTTTCTTTTTCTTTTTTTTTTTG1000.12959243180198277No Hit
GTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTTGATTGTAGAT980.1270005831659431No Hit
CTCTCACAGTGTACTCTATAAGAGGTGTGGGTGTCTGTTTGGTCAGGATG970.12570465884792328No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA970.12570465884792328No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA960.12440873452990345No Hit
GTGTACTCTATAAGAGGTGTGGGTGTCTGTTTGGTCAGGATGTTAGAAAG940.1218168858938638No Hit
GTGTGATGCCATATCAAGTCCATGTTATTCTCTCACAGTGTACTCTATAA930.12052096157584398No Hit
GTCCAAATAGCATAACCTAATTGCATTCAAAACCATTTTCAAATCCATCT920.11922503725782414No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT920.11922503725782414No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAACCTCGTATGCCGT910.1179291129398043No Hit
GTTAGAAAGTGCTGATAAGTAGCATGATCAGTGTATGCGAAAAGGTTTTT910.1179291129398043No Hit
CCTCATGGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTT910.1179291129398043No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT900.11663318862178448No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT900.11663318862178448No Hit
GCATAACCTAATTGCATTCAAAACCATTTTCAAATCCATCTTTAAACTAG860.11144949134970518No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTTTTCTTTTTCTTTTTTTTTTT850.11015356703168536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA840.10885764271366552No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA840.10885764271366552No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG840.10885764271366552No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG830.10756171839564568No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA830.10756171839564568No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT820.10626579407762586No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG820.10626579407762586No Hit
TGCCTGTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTTGATTG800.10367394544158622No Hit
GTACATGGGATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTT790.10237802112356639No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC790.10237802112356639No Hit
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC790.10237802112356639No Hit
GGATGTTAGAAAGTGCTGATAAGTAGCATGATCAGTGTATGCGAAAAGGT790.10237802112356639No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)