FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77165
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAAGAGGTGTGGGTGTCTGTTTGGTCAGGATGTTAGAAAGTGCTGAT4360.5650230026566448No Hit
ACCTAATTGCATTCAAAACCATTTTCAAATCCATCTTTAAACTAGTCAGA3130.40562431154020606No Hit
ATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTC2780.3602669604095121No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2650.3434199442752543No Hit
TCTTTAAACTAGTCAGAAAACAGGTTATTATTTTTTTAAATCACTTAACA2430.3149096092788181No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG2260.29287889587248106No Hit
GACTATATCATGTCACCATCATAGCCAATACAACATTTTTGCCATACTTC2160.27991965269228275No Hit
CTTTAATAGCAAGCGAATGGTAATTACATGGTCGGATGAGGTCCTCACTC2150.2786237283742629No Hit
GATAAGACCTCTTAAAAGGCAGCTGACTATATCATGTCACCATCATAGCC2090.270848182466144No Hit
GTGTATGCGAAAAGGTTTTTAGGAAGTATGGCAAAAATGTTGTATTGGCT1940.25140931769584657No Hit
CTCTATAAGAGGTGTGGGTGTCTGTTTGGTCAGGATGTTAGAAAGTGCTG1910.2475215447417871No Hit
ACTTAACACTGAACAGATAAGACCTCTTAAAAGGCAGCTGACTATATCAT1880.2436337717877276No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA1830.23715415019762848No Hit
GAACAGATAAGACCTCTTAAAAGGCAGCTGACTATATCATGTCACCATCA1730.22419490701743017No Hit
ACACTGAACAGATAAGACCTCTTAAAAGGCAGCTGACTATATCATGTCAC1590.2060519665651526No Hit
GTACATGGGCAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACATAT1560.2021641936110931No Hit
ATCATAGCCAATACAACATTTTTGCCATACTTCCTAAAAACCTTTTCGCA1520.1969804963390138No Hit
CTTAAAAGGCAGCTGACTATATCATGTCACCATCATAGCCAATACAACAT1500.19438864770297415No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG1470.19050087474891467No Hit
GTACTCTATAAGAGGTGTGGGTGTCTGTTTGGTCAGGATGTTAGAAAGTG1270.1645823883885181No Hit
GTATAGGATTTACACTGTTTGAAGCATCTAACGAAGGGCAACAGTTTTTG1260.1632864640704983No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT1180.15291906952633966No Hit
CGCATACACTGATCATGCTACTTATCAGCACTTTCTAACATCCTGACCAA1120.1451435236182207No Hit
GTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTTGATTGTAGAT1090.1412557506641612No Hit
GTTAGAAAGTGCTGATAAGTAGCATGATCAGTGTATGCGAAAAGGTTTTT1070.13866390202812157No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT1050.1360720533920819No Hit
GTGTGATGCCATATCAAGTCCATGTTATTCTCTCACAGTGTACTCTATAA1030.13348020475604225No Hit
GTGTACTCTATAAGAGGTGTGGGTGTCTGTTTGGTCAGGATGTTAGAAAG1020.13218428043802244No Hit
GCCATATCAAGTCCATGTTATTCTCTCACAGTGTACTCTATAAGAGGTGT1010.1308883561200026No Hit
GTCCAAATAGCATAACCTAATTGCATTCAAAACCATTTTCAAATCCATCT1000.12959243180198277No Hit
GTACATGGGATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTT980.1270005831659431No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG940.1218168858938638No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA910.1179291129398043No Hit
CCTCATGGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTT910.1179291129398043No Hit
GCATAACCTAATTGCATTCAAAACCATTTTCAAATCCATCTTTAAACTAG890.11533726430376465No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT860.11144949134970518No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA860.11144949134970518No Hit
CTCTCACAGTGTACTCTATAAGAGGTGTGGGTGTCTGTTTGGTCAGGATG830.10756171839564568No Hit
TGCCTGTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTTGATTG810.10496986975960602No Hit
GTATCAACGCAGAGTACTTTTTTTTTCTTTTCTTTTTCTTTTTTTTTTTG800.10367394544158622No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA780.10108209680554656No Hit
GATATAGTCAGCTGCCTTTTAAGAGGTCTTATCTGTTCAGTGTTAAGTGA780.10108209680554656No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)