FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001421087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420951
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT43351.0298110706471775No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30140.715997823974762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24420.5801150252642231No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT15290.36322517347624783RNA PCR Primer, Index 27 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12710.3019353796522636No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT11780.2798425469947809RNA PCR Primer, Index 27 (96% over 25bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG10290.24444650327472794RNA PCR Primer, Index 27 (96% over 30bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6680.15868830338923057No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4570.10856370456418918Clontech SMART CDS Primer II A (100% over 21bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT4520.10737591786217399RNA PCR Primer, Index 27 (96% over 28bp)
GTACTATAGTGACTCCTGCTGGGAGAGGTAAGTTGAAGTAGCTGAAGGTC4290.10191209903290406No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)