FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001421111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972588
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT49360.5075119166594694No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23410.24069801395863408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19380.199262174733803No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT18020.1852788642261677RNA PCR Primer, Index 39 (95% over 23bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG16420.16882791068777322RNA PCR Primer, Index 39 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT12720.13078508063023603RNA PCR Primer, Index 39 (96% over 25bp)
CTATAAGAGGTGTGGGTGTCTGTTTGGTCAGGATGTTAGAAAGTGCTGAT12440.127906163761017No Hit
ATCAAATACAGTTCTGAGGGTAAGGTCATGGGGGAGCTCGGACCCAGTGG12130.12471879151295306No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)