FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001421113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377305
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24980.6620638475503903No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT24320.6445713679914128No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20050.53140032599621No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10720.28412027404884643No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT7730.2048740408952969RNA PCR Primer, Index 23 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6880.18234584752388652No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG6360.16856389393196486RNA PCR Primer, Index 23 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT5890.15610712818542027RNA PCR Primer, Index 23 (96% over 25bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5530.1465657756987053No Hit
GTCTTATCACATCGCCAATTAGTTGTAATAAACGTTCAACGTACAAACAC4250.11264096685705198No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3830.10150938895588449No Hit
GTATGGAACCTCGGGGGTGACCTGGGAATAGGAGCGTTGAGAGTTCTGTT3810.10097931381773367No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGT3780.10018420111050741No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)