FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20211
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT271313.423383306120428No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT9514.705358468160902TruSeq Adapter, Index 10 (95% over 21bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG7743.8295977438028794TruSeq Adapter, Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT6893.4090346840829255RNA PCR Primer, Index 10 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGT3311.6377220325565287No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA3121.5437138192073623RNA PCR Primer, Index 10 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT2601.286428182672802TruSeq Adapter, Index 10 (96% over 26bp)
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG1630.8064915145217951TruSeq Adapter, Index 10 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGT1250.6184750878234625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.6135272871208747No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.5887882836079362No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC1050.5195190737717085No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTT1030.509623472366533No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC910.45024986393548067Illumina Paired End PCR Primer 2 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT820.4057196576121914No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC760.3760328533966652No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCT730.36118945128890206Illumina PCR Primer Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGACCTCGTATGCCGT710.3512938498837267No Hit
TCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCTGCTTG670.3315026470733759Illumina PCR Primer Index 5 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.3265548463707882No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGT540.26718123793973575No Hit
CTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC440.21770323091385876No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTT400.19791202810350797No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGAGGCT360.1781208252931572No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTTCT350.1731730245905695Illumina PCR Primer Index 5 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC330.1632774231853941No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC330.1632774231853941No Hit
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAA310.15338182178021867TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTTCTGCTTG300.14843402107763098Illumina PCR Primer Index 5 (96% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.13359061896986787Clontech SMART CDS Primer II A (100% over 21bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGAGGC260.1286428182672802No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC250.12369501756469249No Hit
CTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCTGCTTGA240.11874721686210479Illumina PCR Primer Index 5 (96% over 26bp)
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTT240.11874721686210479TruSeq Adapter, Index 10 (96% over 27bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCGAGGCT230.11379941615951708No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC210.10390381475434168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)