FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37721
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT23236.158373319901381No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT8572.2719440099679225RNA PCR Primer, Index 46 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT5771.5296519180297448RNA PCR Primer, Index 46 (95% over 23bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG5511.460724795206914RNA PCR Primer, Index 46 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGT2850.7555473078656452No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT2480.657458710002386RNA PCR Primer, Index 46 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.657458710002386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.49309403250178946No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG1530.4056096073805042TruSeq Adapter, Index 4 (95% over 23bp)
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA1470.38970334826754327RNA PCR Primer, Index 46 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.3207762254447125No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGTCTT920.24389597306540123No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAGCTCGTATGCCGT920.24389597306540123No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC840.2226876275814533TruSeq Adapter, Index 18 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC800.21208345483947932No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC760.20147928209750537No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGTCTTCT650.17231780705707697Illumina PCR Primer Index 8 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.1643646775005965No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAACCTCGTATGCCGT560.14845841838763552No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT560.14845841838763552No Hit
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC480.1272500729036876No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.10339068423424619No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)