FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53216
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT25404.77300060132291No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT9281.7438364401683706RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT6141.1537883343355382RNA PCR Primer, Index 17 (95% over 23bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG5791.0880186410102224RNA PCR Primer, Index 17 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAACTCGTATGCCGT3400.6389055923030668No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2990.5618610944076969No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT2420.4547504509921828RNA PCR Primer, Index 17 (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.4472339146121467No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA1740.32696933253156946RNA PCR Primer, Index 17 (96% over 28bp)
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG1280.24052916416115455TruSeq Adapter, Index 12 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.2273752254960914No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAGCTCGTATGCCGT1000.187913409500902No Hit
GTGTTAGTACAACTGAAGGGATCCAACTTGCAAAAGAACTAGGAGCAACC990.18603427540589298No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGC990.18603427540589298TruSeq Adapter, Index 25 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAAACTCGTATGCCGTCTT980.18415514131088395No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC970.18227600721587495No Hit
GTCTAATTCCATGAAAGGAGTTGCTCATTTTTCTTTTCTTCATTTTTTCA870.16348466626578473No Hit
GTACATGGGAGAGGGAGCTGTGTTAGTACAACTGAAGGGATCCAACTTGC840.15784726398075768No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC810.1522098616957306No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT750.1409350571256765No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAAACTCGTATGCCGTCTTCT710.13341852074564042RNA PCR Primer, Index 19 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAACCTCGTATGCCGT550.10335237522549609No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)