FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63238
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT42916.785477086561878No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT14902.3561782472563966RNA PCR Primer, Index 31 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT10311.6303488408868088RNA PCR Primer, Index 31 (95% over 24bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG4700.7432240108795345RNA PCR Primer, Index 31 (96% over 29bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT3780.5977418640690724RNA PCR Primer, Index 31 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.5835099149245707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3620.5724406211455138No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGT2970.4696543217685568No Hit
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG2230.3526360732470983RNA PCR Primer, Index 38 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1730.2735696891109776No Hit
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC1700.26882570606281037No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC1670.2640817230146431No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG1660.2625003953319207No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT1330.21031658180208102No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTAGCTCGTATGCCGT1310.2071539264366362No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA1310.2071539264366362No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCC1280.20240994338846896No Hit
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA1250.1976659603403017RNA PCR Primer, Index 31 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT1240.1960846326575793RNA PCR Primer, Index 31 (95% over 21bp)
ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC1210.19134064960941205RNA PCR Primer, Index 38 (96% over 25bp)
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC1140.18027135583035517No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA1110.17552737278218794No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGTCTT1090.17236471741674309No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC1060.16762073436857586No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTACCTCGTATGCCGT1050.16603940668585346No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA1050.16603940668585346No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG1040.16445807900313103No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.15813276827224138No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG970.15338878522407415No Hit
GTACATGGGCACCCAACAATGACTAATCAAACTAACCTCAAAACAAATGA940.1486448021759069No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA940.1486448021759069No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA870.13757550839684998No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA870.13757550839684998No Hit
GATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTAC850.13441285303140518No Hit
TTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTA830.13125019766596036No Hit
GTAATAAGCAGTGCTTGAATTATTTGGTTTCGGTTGTTTTCTATTAGACT810.1280875423005155No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA800.1265062146177931No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT790.12492488693507067No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGTCTTCT790.12492488693507067RNA PCR Primer, Index 38 (95% over 22bp)
CAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAA770.12176223156962586No Hit
GTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTTTAATCTTA740.11701824852145863No Hit
TCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATA720.11385559315601379No Hit
CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC690.10911161010784655No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC680.10753028242512412No Hit
CCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACT680.10753028242512412No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG670.10594895474240172No Hit
TTCTAGGATAGTCAGTAGAATTAGAATTGTGAAGATGATAAGTGTAGAGG660.10436762705967931No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT650.10278629937695688No Hit
GTACATGGGATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTC650.10278629937695688No Hit
CCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTC650.10278629937695688No Hit
GGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTTTAATCT640.10120497169423448No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.10120497169423448No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCCATCTCGTATGCCGT640.10120497169423448No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)