FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63238
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2080.32891615800626206No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA1570.24826844618741897No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT1480.23403649704291726No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC1470.23245516936019484No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC1470.23245516936019484No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA1280.20240994338846896No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG1230.19450330497485688No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA1060.16762073436857586No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA1010.15971409595496378No Hit
GTACATGGGCACCCAACAATGACTAATCAAACTAACCTCAAAACAAATGA1000.15813276827224138No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG990.15655144058951898No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG960.15180745754135172No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA950.1502261298586293No Hit
GTAATAAGCAGTGCTTGAATTATTTGGTTTCGGTTGTTTTCTATTAGACT940.1486448021759069No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC900.14231949144501724No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA890.1407381637622948No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT870.13757550839684998No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG810.1280875423005155No Hit
GTACATGGGATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTC790.12492488693507067No Hit
CCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACT760.12018090388690343No Hit
AGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAG750.11859957620418103No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC750.11859957620418103No Hit
TTCTAGGATAGTCAGTAGAATTAGAATTGTGAAGATGATAAGTGTAGAGG740.11701824852145863No Hit
TCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATA720.11385559315601379No Hit
CTCATAAGGGGATGGCCATGGCTAGGTTTATAGATAGTTGGGTGGTTGGT720.11385559315601379No Hit
GCAGTAATGTTAGCGGTTAGGCGTACGGCCAGGGCTATTGGTTGAATGAG720.11385559315601379No Hit
GATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTAC700.11069293779056896No Hit
CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA700.11069293779056896No Hit
ATATTAGTTGGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATG690.10911161010784655No Hit
GGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATTAGGTGCATGAGT680.10753028242512412No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA680.10753028242512412No Hit
GCCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTT650.10278629937695688No Hit
TTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTA650.10278629937695688No Hit
ACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTT640.10120497169423448No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)