FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59899
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT16322.7245863870849263No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11211.8714836641680161No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9611.6043673517087096No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9391.567638858745555No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT5810.9699661096178567TruSeq Adapter, Index 10 (95% over 21bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG5120.8547721998697808TruSeq Adapter, Index 10 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4980.8313995225295915No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.8096963221422728No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT4640.7746373061319889RNA PCR Primer, Index 10 (95% over 23bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.4190387151705371No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.3288869597155211No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA1930.3222090519040385RNA PCR Primer, Index 10 (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.2871500358937545No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT1610.2687857894121771TruSeq Adapter, Index 10 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGT1460.2437436351191172No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.24040468121337585Clontech SMART CDS Primer II A (100% over 21bp)
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC1430.2387352042605052No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT1390.2320572964490225No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA1210.20200671129735054No Hit
GTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTTGATTGTAGAT1150.19198984958012655No Hit
GGCTTATGCAGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC1080.18030351091003188No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA1040.1736256030985492No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC1030.17195612614567857No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA1010.16861717223993722No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG980.16360874138132525TruSeq Adapter, Index 10 (95% over 24bp)
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC980.16360874138132525No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCAGAGGAG920.15359187966410123No Hit
TGCCTGTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTTGATTG910.15192240271123056No Hit
GTATTAATATTTTAAATGAATCACTCCTTTTCCTTTCCTCCAATGTCCCA880.14691397185261856No Hit
CCTCTTGACTGTGGCATAGAGTTATTTCTCCAAACAAGCACGCGGCATTT870.14524449489974792No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.13856658708826525No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT810.1352276331825239No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA790.13188867927678258No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.12854972537104126No Hit
CATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGT750.12521077146529994No Hit
GTACATGGGGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAA740.12354129451242926No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGT720.12020234060668793No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC720.12020234060668793No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA700.1168633867009466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC700.1168633867009466No Hit
GAGCATGCCTGTGTTGGGTTGACAGTGGGGGTAATAATGACTTGTTGGTT700.1168633867009466No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC700.1168633867009466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG690.11519390974807592No Hit
GTCTTGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCAC690.11519390974807592No Hit
CTCCTGGACCCAGCGCCTGCACTCCGTGGGGCGCCATTCGGGTTGCTTGC680.11352443279520526No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT680.11352443279520526No Hit
ACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA670.1118549558423346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC660.11018547888946392No Hit
CCTTATGAGCATGCCTGTGTTGGGTTGACAGTGGGGGTAATAATGACTTG660.11018547888946392No Hit
TTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGGGGGTAATAATGACT640.1068465249837226No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC630.10517704803085194No Hit
TTTCAGACCATCCCTTCTGAGAGAGGAGGCAGCGTGGTGGAGGGAAGAGC630.10517704803085194No Hit
CCCTAGTAGCTGGAATTACAGGCACCCGCCACCATGCCCAGCTAATTTTT620.10350757107798127No Hit
GTACATGGGTAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTC610.1018380941251106No Hit
GTACATGGGCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAGAACCCT600.10016861717223995No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC600.10016861717223995No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)